Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,153,648	A→G	Y29C (TAC→TGC)	yghB →	DedA family protein YghB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,153,648	0	A	G	100.0%	225.8 / NA	62	Y29C (TAC→TGC)	yghB	DedA family protein YghB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/25);  total (37/25)

CAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTACTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATT  >  NC_000913/3153511‑3153784                                                                                                                                          |                                                                                                                                         cAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCt                                                                                                                                        <  1:246079/140‑1 (MQ=255)         cAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATg                                                                                                                                >  1:63233/1‑140 (MQ=255)         cAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATg                                                                                                                                >  2:212783/1‑140 (MQ=255)          aaTAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGt                                                                                                                               >  1:369765/1‑140 (MQ=255)              ggCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGc                                                                                                                           >  2:518223/1‑140 (MQ=255)               gCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGcc                                                                                                                          >  2:160109/1‑140 (MQ=255)                 aGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCAc                                                                                                                        >  1:840356/1‑140 (MQ=255)                 aGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCAc                                                                                                                        >  2:461500/1‑140 (MQ=255)                  gATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACg                                                                                                                       <  2:498708/140‑1 (MQ=255)                  gATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACg                                                                                                                       <  2:919807/140‑1 (MQ=255)                   aTAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGc                                                                                                                      <  2:63233/140‑1 (MQ=255)                    tAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCt                                                                                                                     >  1:302749/1‑140 (MQ=255)                           ttAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTa                                                                                                              >  1:384638/1‑140 (MQ=255)                             aaaCGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGa                                                                                                            >  1:202971/1‑140 (MQ=255)                             aaaCGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATCTTTGCCACGCTGTTTTTAGa                                                                                                            >  2:54868/1‑140 (MQ=255)                                   tGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACgg                                                                                                      >  2:513590/1‑140 (MQ=255)                                          caGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCctgctg                                                                                               <  1:513590/140‑1 (MQ=255)                                               aaGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGc                                                                                          >  2:351632/1‑140 (MQ=255)                                               aaGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGc                                                                                          >  2:353025/1‑140 (MQ=255)                                               aaGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGc                                                                                          >  1:43451/1‑140 (MQ=255)                                               aaGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGc                                                                                          >  1:240989/1‑140 (MQ=255)                                                  tCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTc                                                                                       >  2:487720/1‑140 (MQ=255)                                                    cATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCAt                                                                                     >  1:830303/1‑140 (MQ=255)                                                       ggCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCAtttt                                                                                  >  1:806390/1‑140 (MQ=255)                                                         cTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTg                                                                                <  1:518223/140‑1 (MQ=255)                                                          tGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGc                                                                               <  2:840356/140‑1 (MQ=255)                                                           gTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGcc                                                                              <  2:202971/140‑1 (MQ=255)                                                            ttATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCa                                                                             <  1:455797/140‑1 (MQ=255)                                                                 cAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGa                                                                        <  2:240989/140‑1 (MQ=255)                                                                 cAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGa                                                                        <  1:160109/140‑1 (MQ=255)                                                                   aGATATCATCGCTGCGCTCTGGCAAGACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACa                                                                      >  1:275951/1‑140 (MQ=255)                                                                     atatCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGc                                                                    <  2:43451/140‑1 (MQ=255)                                                                      tatCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCt                                                                   <  2:369765/140‑1 (MQ=255)                                                                       atcatcGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCtt                                                                  <  2:384638/140‑1 (MQ=255)                                                                         catcGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCttgt                                                                >  1:310431/1‑140 (MQ=255)                                                                         catcGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCttgt                                                                >  2:881264/1‑140 (MQ=255)                                                                         catcGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCttgt                                                                >  1:436015/1‑140 (MQ=255)                                                                           tcGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCttgttg                                                              <  2:302749/140‑1 (MQ=255)                                                                              cTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATa                                                           >  1:143540/1‑140 (MQ=255)                                                                              cTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATa                                                           >  1:404770/1‑140 (MQ=255)                                                                              cTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATa                                                           >  1:435650/1‑140 (MQ=255)                                                                                  gcTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTgg                                                       <  1:353025/140‑1 (MQ=255)                                                                                   ctctGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGc                                                      >  1:825372/1‑140 (MQ=255)                                                                                        gCAACCCGCTTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTGGTCATGTTTGCCCCGCTTTTTTTAGAAACCGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGgcg                                                 <  2:469127/140‑1 (MQ=255)                                                                                              cGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCattga                                           <  1:708192/140‑1 (MQ=255)                                                                                              cGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCattga                                           <  2:825372/140‑1 (MQ=255)                                                                                              cGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCattga                                           <  2:435650/140‑1 (MQ=255)                                                                                              cGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATCTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCattga                                           <  1:54868/140‑1 (MQ=255)                                                                                                  tttGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGc                                       >  1:252830/1‑140 (MQ=255)                                                                                                       cgcgCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAgg                                  <  2:275951/140‑1 (MQ=255)                                                                                                       cgcgCTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAgg                                  <  1:838104/140‑1 (MQ=255)                                                                                                           cTGGCGGATCCTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGt                              >  1:855530/1‑140 (MQ=255)                                                                                                                     cTCATATTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGAtttt                    >  2:479637/1‑140 (MQ=255)                                                                                                                        atatTGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTg                 >  1:811931/1‑140 (MQ=255)                                                                                                                            tGTTAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTa             >  2:778162/1‑140 (MQ=255)                                                                                                                              ttAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGcc               <  2:425760/136‑1 (MQ=255)                                                                                                                              ttAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGcc               >  1:425760/1‑136 (MQ=255)                                                                                                                               tAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGa          >  2:573957/1‑140 (MQ=255)                                                                                                                               tAGCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGa          >  2:9934/1‑140 (MQ=255)                                                                                                                                 gCGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGAtt        >  2:687289/1‑140 (MQ=255)                                                                                                                                  cGTTGTTTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTg       <  2:104688/140‑1 (MQ=255)                                                                                                                                    ttgttTGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCg     >  1:734020/1‑140 (MQ=255)                                                                                                                                       tttGCTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGAtt  <  1:573957/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTACTTTGTCATGTTTGCCACGCTGTTTTTAGAAAACGGCCTGCTGCCCGCCTCATTTTTGCCAGGCGACAGCTTGTTGATACTGGCAGGCGCATTGATTGCCCAGGGGGTTATGGATTTTCTGCCTACGATTGCGATT  >  NC_000913/3153511‑3153784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑