Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,200,486	T→C	E160E (GAA→GAG)	ygiF ←	inorganic triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,200,486	0	T	C	100.0%	174.8 / NA	48	E160E (GAA→GAG)	ygiF	inorganic triphosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/23);  total (25/23)

ACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGC  >  NC_000913/3200351‑3200622                                                                                                                                        |                                                                                                                                         aCGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAgttccagctcca                                                                                                                                      <  2:758861/140‑1 (MQ=255)  cgcgCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGttccagctccag                                                                                                                                     >  1:337797/1‑140 (MQ=255)   gcgcCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTtccagctccagc                                                                                                                                    <  2:211345/140‑1 (MQ=255)    cgcCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTccagctccagct                                                                                                                                   <  2:337797/140‑1 (MQ=255)    cgcCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTccagctccagct                                                                                                                                   <  1:206493/140‑1 (MQ=255)             gCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAg                                                                                                                          <  1:737941/140‑1 (MQ=255)             gCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAg                                                                                                                          <  1:247177/140‑1 (MQ=255)              cccTGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAgg                                                                                                                         <  2:790/140‑1 (MQ=255)                 tGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTc                                                                                                                      >  2:656807/1‑140 (MQ=255)                   cgcCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAg                                                                                                                    >  2:85189/1‑140 (MQ=255)                   cgcCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAg                                                                                                                    >  2:191947/1‑140 (MQ=255)                   cgcCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAg                                                                                                                    >  1:689712/1‑140 (MQ=255)                        gatgatAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAAt                                                                                                               >  1:809171/1‑140 (MQ=255)                             tAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCAcc                                                                                                          >  2:40530/1‑140 (MQ=255)                             tAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCCCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCAcc                                                                                                          >  1:353409/1‑140 (MQ=255)                                 cACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCt                                                                                                      <  2:126047/140‑1 (MQ=255)                                  aCGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCtt                                                                                                     <  2:225655/140‑1 (MQ=255)                                   cgcgCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCttt                                                                                                    >  2:854637/1‑140 (MQ=255)                                    gcgcCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTc                                                                                                   >  2:917876/1‑140 (MQ=255)                                            ttGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTcccc                                                                                           <  1:191947/140‑1 (MQ=255)                                             tGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTcccct                                                                                          >  2:644763/1‑139 (MQ=255)                                             tGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTccccc                                                                                          <  2:271494/140‑1 (MQ=255)                                             tGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTccccc                                                                                          >  2:405351/1‑140 (MQ=255)                                                   ggCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCg                                                                                    <  2:248838/140‑1 (MQ=255)                                                     cTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAg                                                                                  <  1:724759/140‑1 (MQ=255)                                                       gCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAggg                                                                                <  1:667634/140‑1 (MQ=255)                                                         ccTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCg                                                                              >  1:414297/1‑140 (MQ=255)                                                                 aaTCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAAt                                                                      <  1:432242/140‑1 (MQ=255)                                                                 aaTCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAAt                                                                      <  2:414297/140‑1 (MQ=255)                                                                  aTCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCGAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAAtt                                                                     >  2:800510/1‑140 (MQ=255)                                                                  aTCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAAtt                                                                     >  2:785846/1‑140 (MQ=255)                                                                       gTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCt                                                                >  2:392907/1‑140 (MQ=255)                                                                            cGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCAt                                                           <  2:353679/140‑1 (MQ=255)                                                                            cGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCAt                                                           <  1:85189/140‑1 (MQ=255)                                                                                aCCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGAc                                                       <  1:656807/140‑1 (MQ=255)                                                                                           cGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGcc                                            >  2:75532/1‑140 (MQ=255)                                                                                           cGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGcc                                            >  1:226219/1‑140 (MQ=255)                                                                                                           gcgcgcgTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATaaaa                            >  2:269470/1‑140 (MQ=255)                                                                                                           gcgcgcgTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATaaaa                            >  2:837457/1‑140 (MQ=255)                                                                                                            cgcgcgTGTCGCCGCTAAGCAGTTCCTGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTT‑CCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAAt                          <  1:644763/140‑1 (MQ=255)                                                                                                            cgcgcgTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATaaaaa                           >  2:189707/1‑140 (MQ=255)                                                                                                            cgcgcgTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATaaaaa                           >  1:576760/1‑140 (MQ=255)                                                                                                            cgcgcgTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATaaaaa                           >  2:857731/1‑140 (MQ=255)                                                                                                            cgcgcgTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGACaaaaa                           >  1:832797/1‑140 (MQ=255)                                                                                                            cgcgcgTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACCTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATaaaaa                           >  1:171398/1‑140 (MQ=255)                                                                                                             gcgcgTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAAt                          <  1:857731/140‑1 (MQ=255)                                                                                                             gcgcgTGTCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAAt                          <  2:809171/140‑1 (MQ=255)                                                                                                                  tgtCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTg                     <  1:40530/140‑1 (MQ=255)                                                                                                                   gtCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGc                    <  1:189707/140‑1 (MQ=255)                                                                                                                   gtCGCCGCTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGc                    <  1:118072/140‑1 (MQ=255)                                                                                                                       ccgcTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACCTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGaa                <  2:171398/140‑1 (MQ=255)                                                                                                                         ccTAAGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACa              <  2:179752/139‑1 (MQ=255)                                                                                                                             aGCAGTTCCAGCTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCgg          >  2:105308/1‑140 (MQ=255)                                                                                                                                  ttccagctccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCa     >  2:373351/1‑140 (MQ=255)                                                                                                                                  ttccagctccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCa     <  1:854637/140‑1 (MQ=255)                                                                                                                                     cagctccagctcACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCAcgc  <  1:338161/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         ACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGC  >  NC_000913/3200351‑3200622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑