Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,322,459	A→G	L16P (CTT→CCT)	secG ←	Sec translocon subunit SecG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,322,459	0	A	G	100.0%	167.8 / NA	45	L16P (CTT→CCT)	secG	Sec translocon subunit SecG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/20);  total (25/20)

CGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGT  >  NC_000913/3322323‑3322593                                                                                                                                         |                                                                                                                                       cGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGc                                                                                                                                     >  2:745459/1‑140 (MQ=255) cGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGc                                                                                                                                     >  1:69353/1‑140 (MQ=255)      ccAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCACCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAAtt                                                                                                                                >  2:838523/1‑140 (MQ=255)          cagcGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCca                                                                                                                            <  1:231882/140‑1 (MQ=255)            gcgcCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCcaca                                                                                                                          >  1:38558/1‑140 (MQ=255)             cgcCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACaa                                                                                                                         >  1:677914/1‑140 (MQ=255)                     tGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGaaa                                                                                                                 <  1:221120/140‑1 (MQ=255)                             aTGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTaa                                                                                                         >  2:847281/1‑140 (MQ=255)                               gAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTaaaa                                                                                                       <  2:728038/140‑1 (MQ=255)                               gAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTaaaa                                                                                                       <  1:838523/140‑1 (MQ=255)                                 aGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAaga                                                                                                     <  1:354816/140‑1 (MQ=255)                                    tACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCt                                                                                                  <  2:69353/140‑1 (MQ=255)                                     aCCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCtt                                                                                                 <  2:95897/140‑1 (MQ=255)                                     aCCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCtt                                                                                                 <  2:724913/140‑1 (MQ=255)                                     aCCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCtt                                                                                                 <  1:48147/140‑1 (MQ=255)                                     aCCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCtt                                                                                                 >  1:496931/1‑140 (MQ=255)                                          aaCCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGTAAACTACTAAAAGAGCTTcatac                                                                                            >  1:230293/1‑140 (MQ=255)                                          aaCCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAact                                                                                                              >  1:434763/1‑122 (MQ=255)                                          aaCCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAact                                                                                                              <  2:434763/122‑1 (MQ=255)                                               cTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAAt                                                                                       >  2:478957/1‑140 (MQ=255)                                                    aCCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAAcc                                                                                  >  2:280044/1‑140 (MQ=255)                                                         aCAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTc                                                                             >  2:916809/1‑140 (MQ=255)                                                                          ccTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCcgcg                                                            <  2:496931/140‑1 (MQ=255)                                                                          ccTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCcgcg                                                            >  2:700883/1‑140 (MQ=255)                                                                          ccTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCATCATACATAATCAACCTGTTCCTTGCGGAGTTGCcgcg                                                            <  1:745459/140‑1 (MQ=255)                                                                                    aggaggCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCt                                                  <  2:753875/140‑1 (MQ=255)                                                                                    aggaggCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCt                                                  <  1:758323/140‑1 (MQ=255)                                                                                      gaggCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTc                                                <  2:820578/140‑1 (MQ=255)                                                                                           tCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCa                                           >  1:511649/1‑140 (MQ=255)                                                                                             ccATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAAt                                         >  2:634996/1‑140 (MQ=255)                                                                                               atatCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATaa                                       >  1:865463/1‑140 (MQ=255)                                                                                                       gcCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAg                               >  2:189885/1‑140 (MQ=255)                                                                                                       gcCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAg                               >  2:82281/1‑140 (MQ=255)                                                                                                       gcCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAg                               >  2:821751/1‑140 (MQ=255)                                                                                                             aCCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTa                         <  1:911054/140‑1 (MQ=255)                                                                                                                ttGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATc                      <  1:189885/140‑1 (MQ=255)                                                                                                                      cagcaTGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTccc                >  1:78758/1‑140 (MQ=255)                                                                                                                         caTGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACt             >  2:241543/1‑140 (MQ=255)                                                                                                                             aTCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAg         >  1:290204/1‑140 (MQ=255)                                                                                                                             aTCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAg         <  2:250538/140‑1 (MQ=255)                                                                                                                               cAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCg       <  2:677914/140‑1 (MQ=255)                                                                                                                               cAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCg       <  1:916809/140‑1 (MQ=255)                                                                                                                                   ccAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGgtg   >  2:544499/1‑140 (MQ=255)                                                                                                                                   ccAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGgtg   >  2:36112/1‑140 (MQ=255)                                                                                                                                   ccAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACAAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGgtg   >  1:920104/1‑140 (MQ=255)                                                                                                                                    caacagGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGgtgt  <  1:26497/140‑1 (MQ=255)                                                                                                                                    caacagGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGgtgt  <  2:865463/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGT  >  NC_000913/3322323‑3322593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑