Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,332,350	A→G	V129A (GTT→GCT)	yhbE ←	inner membrane protein YhbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,332,350	0	A	G	100.0%	127.4 / NA	35	V129A (GTT→GCT)	yhbE	inner membrane protein YhbE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/15);  total (20/15)

GCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAG  >  NC_000913/3332236‑3332481                                                                                                                     |                                                                                                                                    gcACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCttt                                                                                                            <  2:530891/140‑1 (MQ=255)   aCCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGa                                                                                                          <  2:672361/140‑1 (MQ=255)   aCCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGa                                                                                                          >  1:17437/1‑140 (MQ=255)   aCCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGa                                                                                                          <  2:859667/140‑1 (MQ=255)       aCCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGAt                                                                                                      >  2:242524/1‑140 (MQ=255)                 tAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAGGAGAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGc                                                                                              <  1:213155/140‑1 (MQ=255)                   aCTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGc                                                                                          >  1:195633/1‑140 (MQ=255)                   aCTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGc                                                                                          >  2:279387/1‑140 (MQ=255)                            gtgtAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACcatcat                                                                                 >  2:874263/1‑140 (MQ=255)                               tAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGcc                                                                              >  1:632806/1‑140 (MQ=255)                                 aTCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCaa                                                                            >  2:130316/1‑140 (MQ=255)                                 aTCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCaa                                                                            >  1:673902/1‑140 (MQ=255)                                      tGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGc                                                                       >  1:104151/1‑140 (MQ=255)                                      tGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGc                                                                       >  2:322754/1‑140 (MQ=255)                                          cTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCgaga                                                                   <  1:620805/140‑1 (MQ=255)                                                  atatCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCa                                                           <  2:868065/140‑1 (MQ=255)                                                       tcGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCAc                                                      >  1:271133/1‑140 (MQ=255)                                                       tcGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCAc                                                      >  2:287257/1‑140 (MQ=255)                                                             aGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGtt                                                                <  1:799778/124‑1 (MQ=255)                                                             aGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGtt                                                                >  2:799778/1‑124 (MQ=255)                                                              gACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGaa                                               <  1:6731/140‑1 (MQ=255)                                                                   gtgtTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGt                                          <  1:154292/140‑1 (MQ=255)                                                                     gtTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCg                                        <  2:799413/140‑1 (MQ=255)                                                                        aaaaaaCATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGAc                                     <  1:242524/140‑1 (MQ=255)                                                                             aCATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAgg                                <  1:130316/140‑1 (MQ=255)                                                                             aCATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAgg                                <  1:475470/140‑1 (MQ=255)                                                                             aCATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAgg                                <  1:279387/140‑1 (MQ=255)                                                                              cATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGt                               >  2:665039/1‑140 (MQ=255)                                                                              cATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGt                               >  2:130030/1‑140 (MQ=255)                                                                               aTCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTa                              >  2:712735/1‑140 (MQ=255)                                                                                           cTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGa                  >  2:342690/1‑140 (MQ=255)                                                                                           cTCAG‑‑GAGCATCAATGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGa                  >  2:717391/1‑140 (MQ=255)                                                                                                          aTGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGAt                    <  2:486318/125‑1 (MQ=255)                                                                                                          aTGCCCCTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGAt                    >  1:486318/1‑125 (MQ=255)                                                                                                             ccccTACAGCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAg  >  1:585138/1‑140 (MQ=255)                                                                                                                     |                                                                                                                                    GCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAG‑‑GAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAG  >  NC_000913/3332236‑3332481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑