Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,282,845	(T)6→5	intergenic (+44/+95)	rplY → / ← yejK	50S ribosomal subunit protein L25/nucleoid‑associated protein YejK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,282,840	0	T	.	100.0%	109.7 / NA	27	intergenic (+39/+100)	rplY/yejK	50S ribosomal subunit protein L25/nucleoid‑associated protein YejK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (12/15);  total (12/15)

CCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGC  >  NC_000913/2282701‑2282964                                                                                                                                            |                                                                                                                             ccATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGt                                                                                                                              >  2:464888/1‑140 (MQ=255)     cGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGttttt                                                                                                                          >  1:936758/1‑140 (MQ=255)     cGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGttttt                                                                                                                          >  1:1379591/1‑140 (MQ=255)     cGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGttttt                                                                                                                          >  2:1216161/1‑140 (MQ=255)        tgttgACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGtttttgta                                                                                                                       <  2:1262886/140‑4 (MQ=255)            gACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTg                                                                                                                  <  1:551125/140‑1 (MQ=255)                 tAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTg                                                                                                                  <  1:1053235/135‑1 (MQ=255)                 tAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTg                                                                                                                  >  2:1053235/1‑135 (MQ=255)                 tAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGAt                                                                                                             >  2:602463/1‑140 (MQ=255)                    aGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGa                                                                                                              >  2:1177860/1‑136 (MQ=255)                    aGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGa                                                                                                              <  1:1177860/136‑1 (MQ=255)                      aaaTCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGc                                                                                                        <  1:194828/140‑1 (MQ=255)                      aaaTCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGc                                                                                                        <  1:610224/140‑1 (MQ=255)                       aaTCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGcc                                                                                                       <  1:602463/140‑1 (MQ=255)                             aGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTa                                                                                                 <  1:464888/140‑1 (MQ=255)                             aGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTa                                                                                                 <  1:1464431/140‑1 (MQ=255)                                tAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACgg                                                                                              <  1:1216161/140‑1 (MQ=255)                                              tACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAtttttt                                                                                <  2:1403269/140‑1 (MQ=255)                                                    gTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGc                                                                          <  1:618736/140‑1 (MQ=255)                                                    gTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGc                                                                          >  1:510643/1‑140 (MQ=255)                                                      cACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGc                                                                          >  1:640129/1‑138 (MQ=255)                                                      cACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGc                                                                          <  2:640129/138‑1 (MQ=255)                                                        cccGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGa                                                                      >  1:1099873/1‑140 (MQ=255)                                                                 aCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGtt                                                             >  1:968644/1‑140 (MQ=255)                                                                                               cgcTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACaaaa                               >  2:113391/1‑140 (MQ=255)                                                                                                            aaTAAGTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgcc                  >  1:176236/1‑140 (MQ=255)                                                                                                                 gTTGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAg             >  2:1448451/1‑140 (MQ=255)                                                                                                                  ttGTAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGa            >  1:524051/1‑140 (MQ=255)                                                                                                                      aaaaaaCCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTc        <  2:176236/140‑1 (MQ=255)                                                                                                                      aaaaaaCCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTc        <  1:1459537/140‑1 (MQ=255)                                                                                                                           aCCCCGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGgcg   <  2:510643/140‑1 (MQ=255)                                                                                                                            ccccGCTCCGGCGGGG‑TTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGgcgc  >  2:1285250/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                             CCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGC  >  NC_000913/2282701‑2282964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑