Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,949,993	(T)5→6	coding (752/1332 nt)	argA →	N‑acetylglutamate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,949,988	1	.	T	100.0%	297.7 / NA	84	R249R (CGC→CGT)	argA	N‑acetylglutamate synthase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (44/40);  total (44/40)

GAAAAGATGATTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGA  >  NC_000913/2949848‑2950115                                                                                                                                              |                                                                                                                                 gAAAAGATGATTGG‑TTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTgcgc                                                                                                                                   >  1:160633/1‑140 (MQ=255)           ttGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑‑ttttttgcg                                                                                                                        <  2:52987/140‑5 (MQ=255)              gTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTgg                                                                                                                      <  2:803290/140‑1 (MQ=255)               tttttGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGgc                                                                                                                     <  1:1242073/140‑1 (MQ=255)                ttttGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGgcg                                                                                                                    <  1:308532/140‑1 (MQ=255)                             aGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTg                                                                                                       >  2:1060447/1‑140 (MQ=255)                               ggCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGgcg                                                                                                                    >  1:278644/1‑125 (MQ=255)                               ggCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGgcg                                                                                                                    <  2:278644/125‑1 (MQ=255)                               ggCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGcc                                                                                                     >  1:1407932/1‑140 (MQ=255)                                    cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGcc                                                                                                         >  1:30963/1‑131 (MQ=255)                                    cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGcc                                                                                                         <  2:30963/131‑1 (MQ=255)                                    cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCa                                                                                                  <  1:1091876/138‑1 (MQ=255)                                    cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCa                                                                                                  >  2:1091876/1‑138 (MQ=255)                                    cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                >  1:1004255/1‑140 (MQ=255)                                    cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                >  1:997279/1‑140 (MQ=255)                                    cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                >  1:615699/1‑140 (MQ=255)                                    cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                >  2:1199154/1‑140 (MQ=255)                                    cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                >  2:1276390/1‑140 (MQ=255)                                    cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                >  2:164574/1‑140 (MQ=255)                                    cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                >  2:548845/1‑140 (MQ=255)                                    cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                >  2:643689/1‑140 (MQ=255)                                    cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                >  2:866431/1‑140 (MQ=255)                                     aCTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAgcg                                                                                               <  1:1199154/140‑1 (MQ=255)                                     aCTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAgcg                                                                                               >  1:459926/1‑140 (MQ=255)                                         aTGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAgcggcg                                                                                            >  1:1002659/1‑140 (MQ=255)                                         aTGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgt                                                                                           >  2:1461197/1‑140 (MQ=255)                                          tGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtg                                                                                          <  2:160633/140‑1 (MQ=255)                                          tGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtg                                                                                          <  2:997279/140‑1 (MQ=255)                                               acgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCg                                                                                     <  1:866431/140‑1 (MQ=255)                                                cgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGc                                                                                    >  2:350076/1‑140 (MQ=255)                                                 ggTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt                                                                                   >  1:1033913/1‑140 (MQ=255)                                                      tatTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCAt                                                                              <  1:643689/140‑1 (MQ=255)                                                             tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCa                                                                       >  2:1223192/1‑140 (MQ=255)                                                             tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCa                                                                       >  1:102806/1‑140 (MQ=255)                                                               cGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGt                                                                     >  1:967236/1‑140 (MQ=255)                                                                 aaCTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTa                                                                   >  2:1386351/1‑140 (MQ=255)                                                                     tttCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAg                                                               >  1:1018010/1‑140 (MQ=255)                                                                      ttCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAgg                                                              >  2:664287/1‑140 (MQ=255)                                                                      ttCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAgg                                                              >  1:1061615/1‑140 (MQ=255)                                                                              cGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgc                                                      >  1:10126/1‑140 (MQ=255)                                                                              cGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgc                                                      >  1:1313594/1‑140 (MQ=255)                                                                               gAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcg                                                     <  2:845009/140‑1 (MQ=255)                                                                                aagcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgc                                                    <  2:1407932/140‑1 (MQ=255)                                                                                 agcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcg                                                   >  2:596069/1‑140 (MQ=255)                                                                                 agcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcg                                                   <  2:1118235/140‑1 (MQ=255)                                                                                  gcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc                                                  <  1:1276390/140‑1 (MQ=255)                                                                                   cgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCTGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCt                                                 <  2:1198321/140‑1 (MQ=255)                                                                                   cgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAATGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCt                                                 <  2:1018010/140‑1 (MQ=255)                                                                                       agcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc                                                  <  2:98337/135‑1 (MQ=255)                                                                                       agcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc                                                  >  1:98337/1‑135 (MQ=255)                                                                                       agcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTg                                             <  1:397154/140‑1 (MQ=255)                                                                                        gcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGc                                            <  1:1366115/140‑1 (MQ=255)                                                                                        gcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGc                                            <  1:664287/140‑1 (MQ=255)                                                                                         cgcgGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCa                                           <  2:1061615/140‑1 (MQ=255)                                                                                             ggTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAagag                                       >  1:1121727/1‑140 (MQ=255)                                                                                                        aGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcg                            >  1:292934/1‑140 (MQ=255)                                                                                                        aGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcg                            >  2:1253826/1‑140 (MQ=255)                                                                                                         ggAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgc                           >  1:1352273/1‑140 (MQ=255)                                                                                                           aaGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa                         <  2:1313594/140‑1 (MQ=255)                                                                                                           aaGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa                         <  2:10126/140‑1 (MQ=255)                                                                                                           aaGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa                         <  2:619297/140‑1 (MQ=255)                                                                                                           aaGAGAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa                         <  1:1060447/140‑1 (MQ=255)                                                                                                            agagAAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGAc                        <  1:596069/140‑1 (MQ=255)                                                                                                             gagaAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACg                       >  1:985923/1‑140 (MQ=255)                                                                                                              agaAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACgg                      >  1:40256/1‑140 (MQ=255)                                                                                                              agaAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACgg                      >  2:666449/1‑140 (MQ=255)                                                                                                              agaAAGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACgg                      >  1:292178/1‑140 (MQ=255)                                                                                                                aaaGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTa                    <  2:1004255/140‑1 (MQ=255)                                                                                                                 aaGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTAt                   <  2:459926/140‑1 (MQ=255)                                                                                                                  aGGCGATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATc                  >  1:1108029/1‑140 (MQ=255)                                                                                                                      gATTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTa              <  2:1121727/140‑1 (MQ=255)                                                                                                                       aTTACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTAc             >  2:466097/1‑140 (MQ=255)                                                                                                                        ttACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACgg                      <  1:20077/130‑1 (MQ=255)                                                                                                                        ttACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACgg                      >  2:20077/1‑130 (MQ=255)                                                                                                                        ttACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACg            >  2:1076864/1‑140 (MQ=255)                                                                                                                        ttACAACTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACg            <  1:489900/140‑1 (MQ=255)                                                                                                                           cAACTCCGGTACGGTGCGCTTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCa          <  2:1002659/140‑1 (MQ=255)                                                                                                                            aaCTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa        <  1:666449/140‑1 (MQ=255)                                                                                                                            aaCTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa        <  1:1253826/140‑1 (MQ=255)                                                                                                                            aaCTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa        <  1:164574/140‑1 (MQ=255)                                                                                                                            aaCTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa        <  1:548845/140‑1 (MQ=255)                                                                                                                            aaCTCCGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa        <  2:985923/140‑1 (MQ=255)                                                                                                                                 cGGTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATtgtg   <  1:466097/140‑1 (MQ=255)                                                                                                                                  ggTACGGTGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGa  >  1:990958/1‑140 (MQ=255)                                                                                                                                         tGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACg            <  1:1111706/123‑1 (MQ=255)                                                                                                                                         tGCGCT‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACg            >  2:1111706/1‑123 (MQ=255)                                                                                                                                              |                                                                                                                                 GAAAAGATGATTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGA  >  NC_000913/2949848‑2950115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑