Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,330	(G)8→9	noncoding (72/87 nt)	leuQ ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,322	1	.	G	95.5%	213.6 / 3.0	66	noncoding (80/87 nt)	leuQ	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (3/0);  new base G (36/27);  total (39/27)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

ATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAG  >  NC_000913/4606189‑4606459                                                                                                                                       |                                                                                                                                            atCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑gg‑gggg                                                                                                                                     <  2:92746/140‑1 (MQ=255)  tCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑gg‑ggggg                                                                                                                                    <  2:1417539/140‑1 (MQ=255)     ccTCGTTTGGTGCGAGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGACTACCCATTCCGCCACCTTCGCCCAGTGCGAGCTATATGAACGTCGTTTTTGGCGCGA‑‑gg‑gggggggc                                                                                                                                 >  2:1217339/1‑138 (MQ=18)           ttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGaa                                                                                                                            <  2:324050/140‑1 (MQ=255)                   aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGa                                                                                                                             >  1:328980/1‑131 (MQ=255)                   aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGa                                                                                                                             <  2:328980/131‑1 (MQ=255)                   aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCa                                                                                                                      <  1:636003/138‑1 (MQ=255)                   aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCa                                                                                                                      >  2:636003/1‑138 (MQ=255)                    ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCCATTCCCCCACCTTCGCCCAGTTCGAGCAATATCAACGTGGTTTTTGGGGCGAGGGG‑GGGGGGACTTGAACCCCCACg                                                                                                                    <  2:898598/140‑1 (MQ=21)                    ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGG‑GGGGGGACTTGAACCCCCACg                                                                                                                    >  2:1234956/1‑140 (MQ=255)                    ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGaa                                                                                                                            >  1:24295/1‑131 (MQ=255)                    ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGaa                                                                                                                            <  2:24295/131‑1 (MQ=255)                    ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGt                                                                                                                   >  1:821264/1‑140 (MQ=255)                     gggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTc                                                                                                                  <  1:432542/140‑1 (MQ=255)                        ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGa                                                                                                                             <  1:165509/126‑1 (MQ=255)                        ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGa                                                                                                                             >  2:165509/1‑126 (MQ=255)                        ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGt                                                                                                               <  1:1305757/140‑1 (MQ=255)                        ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGt                                                                                                               <  1:159125/140‑1 (MQ=255)                           gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                            <  2:1021913/140‑1 (MQ=255)                           gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                            <  2:821264/140‑1 (MQ=255)                             cTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                                           <  1:1234956/140‑1 (MQ=255)                                gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGacac                                                                                                       >  1:386725/1‑140 (MQ=255)                                gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGacac                                                                                                       >  2:159125/1‑140 (MQ=255)                                 aaCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                                      <  2:386725/140‑1 (MQ=255)                                      ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTc                                                                                                                  >  2:527564/1‑123 (MQ=255)                                      ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTc                                                                                                                  <  1:926623/123‑1 (MQ=255)                                      ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTc                                                                                                                  <  1:527564/123‑1 (MQ=255)                                      ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTc                                                                                                                  >  2:926623/1‑123 (MQ=255)                                                         aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                                   <  2:122422/135‑1 (MQ=255)                                                         aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                                   >  1:122422/1‑135 (MQ=255)                                                             ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                          >  1:493664/1‑140 (MQ=255)                                                                gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                       >  1:648638/1‑140 (MQ=255)                                                                gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                       >  1:312013/1‑140 (MQ=255)                                                                gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                       >  1:1154735/1‑140 (MQ=255)                                                                 aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                      >  2:364519/1‑140 (MQ=255)                                                                 aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                      >  1:803732/1‑140 (MQ=255)                                                                 aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                      <  1:512976/140‑1 (MQ=255)                                                                 aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTCCGA‑‑GGCGGGGGGACTTGAACCCCCACGTCCCTAAGGTCGCTAACACCTGAAGCTAGCGCGACCAGCAACTcct                                                                      >  2:355677/1‑139 (MQ=25)                                                                      aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt                                                                                  >  2:510472/1‑123 (MQ=255)                                                                      aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt                                                                                  <  1:510472/123‑1 (MQ=255)                                                                      aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                 >  1:57315/1‑140 (MQ=255)                                                                      aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGCGCGAGCAATATCAACGTAGTTTTTGGTGCGA‑‑GGCGGGGGGTCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGACGCTAGCGCGACTACTAGTTCCGCCAcc                                                                 >  2:61939/1‑140 (MQ=21)                                                                        cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                            >  2:968289/1‑127 (MQ=255)                                                                        cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                            <  1:968289/127‑1 (MQ=255)                                                                        cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                   >  2:1407809/1‑136 (MQ=255)                                                                        cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                   <  1:1407809/136‑1 (MQ=255)                                                                            tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                                           <  2:648638/140‑1 (MQ=255)                                                                               aCCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAcc                                                       >  2:1044893/1‑140 (MQ=255)                                                                                      ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                                           <  1:244998/130‑1 (MQ=255)                                                                                      ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                                           >  2:244998/1‑130 (MQ=255)                                                                                       cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAtt                                                >  2:684009/1‑140 (MQ=255)                                                                                         ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCt                                              >  1:32419/1‑140 (MQ=255)                                                                                               tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaaa                                        >  1:656508/1‑140 (MQ=255)                                                                                                        gCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTa                               <  2:656508/140‑1 (MQ=255)                                                                                                           aGCAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTActa                            >  1:942046/1‑138 (MQ=255)                                                                                                             cAATATCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                          <  2:803732/140‑1 (MQ=255)                                                                                                                tatCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg                       <  2:1154735/140‑1 (MQ=255)                                                                                                                 atCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg                      >  2:442712/1‑140 (MQ=255)                                                                                                                 atCAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg                      >  2:1019836/1‑140 (MQ=255)                                                                                                                   cAACGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                    <  1:442712/140‑1 (MQ=255)                                                                                                                     aCGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                    >  2:402794/1‑138 (MQ=255)                                                                                                                     aCGTGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                    <  1:402794/138‑1 (MQ=255)                                                                                                                        tGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTc               >  1:854407/1‑140 (MQ=255)                                                                                                                        tGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg                       >  1:1127289/1‑132 (MQ=255)                                                                                                                        tGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg                       <  2:1127289/132‑1 (MQ=255)                                                                                                                        tGGTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTc               >  2:575309/1‑140 (MQ=255)                                                                                                                          gTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa             >  1:1054177/1‑140 (MQ=255)                                                                                                                          gTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa             >  1:624197/1‑140 (MQ=255)                                                                                                                          gTTTTTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa             >  1:73872/1‑140 (MQ=255)                                                                                                                                ggTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGtt       >  1:1037164/1‑140 (MQ=255)                                                                                                                                ggTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGtt       >  1:685657/1‑140 (MQ=255)                                                                                                                                    cGA‑‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAg  >  1:813005/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                            ATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAG  >  NC_000913/4606189‑4606459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑