Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,112,249	(G)6→7	coding (1387/2544 nt)	opgH →	osmoregulated periplasmic glucans (OPGs) biosynthesis protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,112,243	1	.	G	100.0%	107.4 / NA	32	W461G (TGG→GGG)	opgH	osmoregulated periplasmic glucans (OPGs) biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (15/17);  total (15/17)

GGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑GGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCT  >  NC_000913/1112107‑1112367                                                                                                                                           |                                                                                                                             gggACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑ggg                                                                                                                           <  2:803921/140‑1 (MQ=255) gggACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑ggg                                                                                                                           >  1:281961/1‑140 (MQ=255)   gACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑ggggg                                                                                                                         >  2:332796/1‑140 (MQ=255)   gACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑ggggg                                                                                                                         >  2:1156665/1‑140 (MQ=255)      aTAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑gggggggt                                                                                                                      >  2:336657/1‑138 (MQ=255)      aTAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑gggggggt                                                                                                                      >  2:1126134/1‑138 (MQ=255)      aTAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑gggggggt                                                                                                                      >  1:630406/1‑138 (MQ=255)      aTAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGACGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑gggggggt                                                                                                                      <  1:708214/140‑3 (MQ=255)        aatgcgATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑gggggggtct                                                                                                                    >  1:1378027/4‑136 (MQ=255)        aaCGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑gggggggtct                                                                                                                    >  1:881058/1‑136 (MQ=255)        aaCGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑gggggggtct                                                                                                                    >  2:384120/1‑136 (MQ=255)        aaCGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑gggggggtct                                                                                                                    >  1:849215/1‑136 (MQ=255)        aaCGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑gggggggt                                                                                                                      >  2:351843/1‑136 (MQ=255)        aaCGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑gggggggt                                                                                                                      <  1:351843/138‑3 (MQ=255)        aaCGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑gggggg                                                                                                                        <  1:458788/136‑1 (MQ=255)        aaCGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑gggggg                                                                                                                        >  2:458788/1‑136 (MQ=255)         aCGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑gggggggtctg                                                                                                                   >  1:188896/1‑135 (MQ=255)           gcgATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGa                                                                                                                  >  1:125264/1‑140 (MQ=255)           gcgATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGa                                                                                                                  >  1:171078/1‑140 (MQ=255)           gcgATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGCCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGa                                                                                                                  <  2:765349/140‑1 (MQ=255)               ttATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGc                                                                                                              >  1:939197/1‑140 (MQ=255)                tATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCt                                                                                                             <  2:630406/140‑1 (MQ=255)                   ccGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTAc                                                                                                          <  1:332796/140‑1 (MQ=255)                    cgcgTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACg                                                                                                         <  1:384120/140‑1 (MQ=255)                      cgTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGAt                                                                                                       <  2:171078/140‑1 (MQ=255)                              cGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCggg                                                                                               <  2:849215/140‑1 (MQ=255)                              cGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCggg                                                                                               <  2:939197/140‑1 (MQ=255)                                      gAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATg                                                                                       >  2:1226985/1‑140 (MQ=255)                                            tGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAAt                                                                                 <  1:303293/140‑1 (MQ=255)                                            tGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAAt                                                                                 <  2:188896/140‑1 (MQ=255)                                              cgcACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTg                                                                               <  1:305327/140‑1 (MQ=255)                                              cgcACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTg                                                                               >  2:604483/1‑140 (MQ=255)                                                      cTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTaa                                                                       <  2:1058837/140‑1 (MQ=255)                                                               cGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTtga                                                              >  1:393591/1‑140 (MQ=255)                                                                gggCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTtgat                                                             <  2:281961/140‑1 (MQ=255)                                                                     aaGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTaa                                                                       >  2:775214/1‑125 (MQ=255)                                                                     aaGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTaa                                                                       <  1:775214/125‑1 (MQ=255)                                                                      aGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTa                                                       <  2:881058/140‑1 (MQ=255)                                                                      aGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTa                                                       >  2:364654/1‑140 (MQ=255)                                                                                 ccGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGAccg                                            >  2:1418524/1‑140 (MQ=255)                                                                                      tCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGAt                                       <  2:393591/140‑1 (MQ=255)                                                                                         aTCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGt                                    >  2:212248/1‑140 (MQ=255)                                                                                          tCCTGTCACATGCCTTCGGGGAGGGGGGGTTGTTGCGCCGTGCAGGT‑TGGGGGGGTTTGGATTGCTTACGATCCCCGGGGTTCTTATGAAGAATTGCCCCCTACCTTGCTTGATGAGAAAAAACGTGCCCGCCGATGGTg                                   <  2:670026/140‑1 (MQ=255)                                                                                          tCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCCCCGGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTg                                   <  2:125264/140‑1 (MQ=255)                                                                                               tCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACg                              >  1:1427318/1‑140 (MQ=255)                                                                                                    tGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAAc                         <  1:212248/140‑1 (MQ=255)                                                                                                    tGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAAc                         >  1:876362/1‑140 (MQ=255)                                                                                                        ttCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCtga                     >  2:1051648/1‑140 (MQ=255)                                                                                                                   ggcgTTGATGCGCCGGGCGGGTGG‑GGGGGGTCTGGTTTGCTTACGATCCCCGGGTTTTTTATGAAGAATTGCCGCCTAATTTGTTTGATGAGCAAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGt          <  2:1427318/140‑1 (MQ=255)                                                                                                                      gTTGATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTg       >  1:56090/1‑140 (MQ=255)                                                                                                                         gATGCGCCGTGCAGGT‑TGGGGGGGTCTGGATTGCTTACGATCCCCGGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCAAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTc    <  1:919128/140‑1 (MQ=255)                                                                                                                           tGCGCCGTGCAGGT‑TGGGGGGGGCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCt  >  1:272313/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                             GGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGT‑T‑GGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCT  >  NC_000913/1112107‑1112367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑