| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,260,895 | (A)7→8 | intergenic (‑92/+33) | dauA ← / ← prs | aerobic C4‑dicarboxylate transporter DauA/ribose‑phosphate diphosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,260,888 | 1 | . | A | 83.7% | 126.7 / 16.5 | 43 | intergenic (‑85/+40) | dauA/prs | aerobic C4‑dicarboxylate transporter DauA/ribose‑phosphate diphosphokinase |
| Reads supporting (aligned to +/- strand): ref base . (1/6); new base A (26/10); total (27/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.06e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑AC‑A‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTT > NC_000913/1260762‑1261020 | agCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑Cccgc > 2:1464684/1‑140 (MQ=255) cgcgGAAAGGCATCACATGTGAGGAAAATTTTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCGGGTG‑AAGGAGGCATAGGTCATAAAAATGGATATTACAG‑AA‑A‑AAAAAA‑ccccgccg < 1:1112218/140‑6 (MQ=255) aaaGGCATCACATGTGAGGAAAATTTTTTGTTCAAAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCg < 1:861883/140‑1 (MQ=255) aaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAGAAA‑A‑AAAAAA‑CCCGCCGCAGCGGGTc < 2:1182155/124‑1 (MQ=255) aaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTc > 1:1182155/1‑124 (MQ=255) aaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAt > 1:599319/1‑140 (MQ=255) ttttttTCAAAATAGTGTTTAATCGGGGTGCGTTTCATTTGT‑AAATAAATGGGAGGGGAAAGGACGCATATTTATTACAATTGTATTTGACAG‑AA‑A‑AAAAAT‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAgtg < 2:738961/140‑1 (MQ=255) tttCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACAAAAGGGCAGGTG‑AAGGAGGCATAGGTCATAAAAATGGATATTACAG‑AA‑A‑AAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTc < 2:599319/139‑1 (MQ=255) aataatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACAt > 1:958105/1‑140 (MQ=255) ttGTTTCATCGGGGAGCGCATCATCTGTCAATTAAAGGGCAGGTG‑AAGGAGGAATAGGTCATACAAATGGATATTACAGAAA‑A‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCa < 2:1441925/139‑1 (MQ=255) atGTTTCATCCGTGAGCGAATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCa < 1:904086/140‑1 (MQ=255) tttCATCCGTGAGCGCATCATCTGTCAACTAAAGGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑AA‑A‑AAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCagag < 2:1127578/140‑1 (MQ=255) cATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTc < 1:1185813/124‑1 (MQ=255) cATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTc > 2:1185813/1‑124 (MQ=255) cATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCACCGCAGCGGGTCTTTGAGCCGGGTTCGCTTAGAGTTGGAAAATGGCAGAGGTc > 2:224648/1‑140 (MQ=255) tCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGa > 2:1090403/1‑140 (MQ=255) tCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑AC‑AGAAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGa > 2:972065/1‑140 (MQ=255) tGAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttct > 1:603456/1‑140 (MQ=255) gAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttctt > 1:60161/1‑140 (MQ=255) aGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttcttc > 2:1359467/1‑140 (MQ=255) ttcTGTCAACTAAAAGGTAGGAG‑AAGGAGGGATGGGCAATAAAAATGGATATTACAGAAA‑A‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGa < 2:60161/139‑1 (MQ=255) aaCTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacgacg > 1:1008186/1‑140 (MQ=255) aCTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATAcgacga > 2:178103/1‑140 (MQ=255) cTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGaa < 1:972065/140‑1 (MQ=255) aaTGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCg > 2:659852/1‑140 (MQ=255) aaTGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCg > 1:1149174/1‑140 (MQ=255) gggCAGGTG‑AAGGAGGCATAGGTCATAAAAATGGATATTACAA‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGGATCGCt < 2:935745/139‑1 (MQ=255) ggCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCtt > 2:1065128/1‑140 (MQ=255) ggTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGc > 1:716494/1‑140 (MQ=255) g‑AAGGGGGCATAGGTAATACAAATGGATATTAAAGAAA‑A‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAg < 1:1464684/140‑1 (MQ=255) gagggaggTATAGTTAACAGAAATTGATATAGCAGTAA‑A‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAg < 1:954848/137‑1 (MQ=255) aTAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGa > 1:870082/1‑140 (MQ=255) tAGGTCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGAc < 1:178103/140‑1 (MQ=255) aGGTCATAAAAAGGGATATTACAGAAA‑A‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACa < 2:958105/140‑1 (MQ=255) tCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAggg > 2:1182228/1‑140 (MQ=255) tCATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAggg > 1:1127474/1‑140 (MQ=255) cATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGt < 1:1090403/140‑1 (MQ=255) cATACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGt > 1:1141791/1‑140 (MQ=255) aTACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTc < 2:1127474/140‑1 (MQ=255) tACAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCa < 1:1065128/140‑1 (MQ=255) aCAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGTAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGa > 1:1302879/1‑131 (MQ=255) aCAAATGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGTAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGa < 2:1302879/131‑1 (MQ=255) aTGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTa > 2:207389/1‑140 (MQ=255) tGGATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTAc > 1:1222750/1‑140 (MQ=255) ggATATTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACg > 1:1423859/1‑140 (MQ=255) tatTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGt > 2:633897/1‑128 (MQ=255) tatTACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGt < 1:633897/128‑1 (MQ=255) tACAG‑ACAA‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGtt > 2:1228699/1‑140 (MQ=255) | AGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTG‑AAGGAGGCATAGGTCATACAAATGGATATTACAG‑AC‑A‑AAAAAA‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTT > NC_000913/1260762‑1261020 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |