Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	148,589	A→G	A69A (GCT→GCC)	panC ←	pantothenate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	148,589	0	A	G	100.0%	273.9 / NA	80	A69A (GCT→GCC)	panC	pantothenate synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/43);  total (37/43)

AGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGC  >  NC_000913/148455‑148715                                                                                                                                       |                                                                                                                               aGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAg                                                                                                                           <  2:878843/140‑1 (MQ=255)    ccAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATc                                                                                                                        >  2:1426840/1‑140 (MQ=255)     cAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTACATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCt                                                                                                                       >  1:220156/1‑140 (MQ=255)     cAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCGGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCt                                                                                                                       >  1:1072708/1‑140 (MQ=255)     cAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCt                                                                                                                       >  1:302183/1‑140 (MQ=255)     cAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCt                                                                                                                       >  2:153550/1‑140 (MQ=255)      aGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGAt                                                                                                                         <  1:691029/137‑1 (MQ=255)      aGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGAt                                                                                                                         >  2:691029/1‑137 (MQ=255)         aaCGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCg                                                                                                                   <  2:886345/140‑1 (MQ=255)             tCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAGCGGGCCAGATCTTCCGGGCg                                                                                                               >  2:881360/1‑140 (MQ=255)              cAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCgg                                                                                                              <  1:1136291/140‑1 (MQ=255)                aCGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTc                                                                                                            >  2:571696/1‑140 (MQ=255)                aCGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTc                                                                                                            >  2:531447/1‑140 (MQ=255)                   tAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCGGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGaa                                                                                                         <  2:1072708/140‑1 (MQ=255)                   tAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAGCGGGCCAGATCTTCCGGGCGGTCGaa                                                                                                         <  1:881360/140‑1 (MQ=255)                   tAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGaa                                                                                                         <  1:1426840/140‑1 (MQ=255)                    aaGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAAc                                                                                                        <  1:1017462/140‑1 (MQ=255)                         tgGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCAt                                                                                                   <  2:522501/140‑1 (MQ=255)                           ggTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTATTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCg                                                                                                 >  1:472261/1‑140 (MQ=255)                               tCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGtt                                                                                             <  2:1324077/140‑1 (MQ=255)                                        ttCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTTGAACTGCATCGGGTTAACGAAAAt                                                                                    >  1:555166/1‑140 (MQ=255)                                        ttCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAAt                                                                                    >  1:1265789/1‑140 (MQ=255)                                        ttCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAAt                                                                                    >  1:73672/1‑140 (MQ=255)                                        ttCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAAt                                                                                    >  2:97762/1‑140 (MQ=255)                                        ttCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAAt                                                                                    >  2:266189/1‑140 (MQ=255)                                         tCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATa                                                                                   <  2:1275572/140‑1 (MQ=255)                                         tCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATa                                                                                   <  1:419436/140‑1 (MQ=255)                                         tCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATa                                                                                   <  1:713948/140‑1 (MQ=255)                                          cGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGtt                                                                                             <  2:1125633/129‑1 (MQ=255)                                          cGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGtt                                                                                             >  1:1125633/1‑129 (MQ=255)                                           gggTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATc                                                                                                  >  2:1046231/1‑123 (MQ=255)                                           gggTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATc                                                                                                  <  1:1046231/123‑1 (MQ=255)                                           gggTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATa                                                                                   >  2:167246/1‑138 (MQ=255)                                           gggTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATa                                                                                   <  1:167246/138‑1 (MQ=255)                                             gTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTga                                                                               >  1:330512/1‑140 (MQ=255)                                             gTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTga                                                                               >  2:1306380/1‑140 (MQ=255)                                                   ctctTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTgacgacga                                                                         >  2:428796/1‑140 (MQ=255)                                                         tACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACAt                                                                   <  2:73672/140‑1 (MQ=255)                                                           ccGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTgacg                                                                             <  1:1432374/128‑1 (MQ=255)                                                           ccGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTgacg                                                                             >  2:1432374/1‑128 (MQ=255)                                                              aaGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGgcg                                                              <  1:1423571/140‑1 (MQ=255)                                                              aaGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGgcg                                                              <  1:266189/140‑1 (MQ=255)                                                              aaGGGGCGAAAACTAAATCCACTTTACGTTTATTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGgcg                                                              <  2:472261/140‑1 (MQ=255)                                                                ggggCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGcgcg                                                            >  2:120301/1‑140 (MQ=255)                                                                ggggCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGCCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGcgcg                                                            >  2:1301126/1‑140 (MQ=255)                                                                    cGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTTGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCt                                                        <  2:555166/140‑1 (MQ=255)                                                                            aaaTCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCCtc                                                >  1:986633/1‑140 (MQ=255)                                                                             aaTCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTtcg                                               <  2:1265789/140‑1 (MQ=255)                                                                              aTCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTtcgt                                              <  1:531447/140‑1 (MQ=255)                                                                              aTCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTtcgt                                              <  1:571696/140‑1 (MQ=255)                                                                                ccACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCCtcgtcg                                            <  2:986633/140‑1 (MQ=255)                                                                                  gcTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGAc                                          <  2:59808/139‑1 (MQ=255)                                                                                  gcTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGAc                                          <  1:1458845/139‑1 (MQ=255)                                                                                  aCTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGAc                                          <  1:153550/140‑1 (MQ=255)                                                                                  aCTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGAc                                          <  1:1301126/140‑1 (MQ=255)                                                                                     ttACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGAc                                          <  2:1059486/137‑1 (MQ=255)                                                                                     ttACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGAc                                          >  1:1059486/1‑137 (MQ=255)                                                                                      tACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGc                                      <  1:518961/140‑1 (MQ=255)                                                                                        cGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCtt                                    >  2:607871/1‑140 (MQ=255)                                                                                            tgtttAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCata                                <  1:97762/140‑1 (MQ=255)                                                                                                tAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGc                            <  1:428796/140‑1 (MQ=255)                                                                                                   cTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCAt                         >  1:45531/1‑140 (MQ=255)                                                                                                       tcGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTg                     <  2:220156/140‑1 (MQ=255)                                                                                                        cGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGc                    >  2:150789/1‑140 (MQ=255)                                                                                                        cGCAGTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGc                    >  2:81314/1‑140 (MQ=255)                                                                                                            gTCCTCCTGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGt                <  1:81314/140‑1 (MQ=255)                                                                                                              cctcctGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTa              <  2:45531/140‑1 (MQ=255)                                                                                                               ctcctGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTAc             <  1:405877/140‑1 (MQ=255)                                                                                                                tcctGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCa                          >  2:424960/1‑126 (MQ=255)                                                                                                                tcctGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCa                          <  1:424960/126‑1 (MQ=255)                                                                                                                tcctGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTAcc            >  1:58215/1‑140 (MQ=255)                                                                                                                  ctGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCa          <  2:58215/140‑1 (MQ=255)                                                                                                                  ctGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCa          <  2:81711/140‑1 (MQ=255)                                                                                                                  ctGCAAGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCa          <  1:1393968/140‑1 (MQ=255)                                                                                                                       aGGTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTa     >  2:772985/1‑140 (MQ=255)                                                                                                                         gTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAgg   >  2:970674/1‑140 (MQ=255)                                                                                                                         gTCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAgg   >  1:459232/1‑140 (MQ=255)                                                                                                                          tCCGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGc  >  1:975398/1‑140 (MQ=255)                                                                                                                            cGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAgg   >  1:1164210/1‑137 (MQ=255)                                                                                                                            cGTGGATAACGGGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAgg   <  2:1164210/137‑1 (MQ=255)                                                                                                                                       |                                                                                                                               AGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGC  >  NC_000913/148455‑148715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑