Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,494,217	T→C	V62V (GTA→GTG)	yfdX ←	protein YfdX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,494,217	0	T	C	98.9%	294.4 / ‑5.1	89	V62V (GTA→GTG)	yfdX	protein YfdX
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (44/44);  total (44/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAG  >  NC_000913/2494080‑2494350                                                                                                                                          |                                                                                                                                      tcatcaTTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCAc                                                                                                                                     >  1:244620/1‑140 (MQ=255) tcatcaTTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCAc                                                                                                                                     >  2:516504/1‑140 (MQ=255)   atcatTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCt                                                                                                                                   <  1:562410/140‑1 (MQ=255)   atcatTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCt                                                                                                                                   >  1:75496/1‑140 (MQ=255)   atcatTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCt                                                                                                                                   >  1:1438569/1‑140 (MQ=255)     catTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGc                                                                                                                                 >  2:651760/1‑140 (MQ=255)     catTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGc                                                                                                                                 <  2:92961/140‑1 (MQ=255)         gagaTTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGt                                                                                                                             <  2:617688/140‑1 (MQ=255)         gagaTTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGt                                                                                                                             <  2:948042/140‑1 (MQ=255)          agaTTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTc                                                                                                                            >  1:1191202/1‑140 (MQ=255)          agaTTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTc                                                                                                                            >  1:1048341/1‑140 (MQ=255)          agaTTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTc                                                                                                                            >  2:1308792/1‑140 (MQ=255)               ggTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGTCGCGCCACCTGCACGTCAcgca                                                                                                                       >  2:683982/1‑140 (MQ=255)               ggTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCAcgca                                                                                                                       >  1:428780/1‑140 (MQ=255)                gTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCAcgcat                                                                                                                      <  1:444793/140‑1 (MQ=255)                gTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCAcgcat                                                                                                                      >  1:118535/1‑140 (MQ=255)                gTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCAcgcat                                                                                                                      <  2:244620/140‑1 (MQ=255)                gTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCAcgcat                                                                                                                      >  1:846096/1‑140 (MQ=255)                 tCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCAcgcatc                                                                                                                     <  1:1113225/140‑1 (MQ=255)                 tCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCAcgcatc                                                                                                                     <  2:1029409/140‑1 (MQ=255)                 tCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCAcgcatc                                                                                                                     <  1:1121661/140‑1 (MQ=255)                  cTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCAcgcatcg                                                                                                                    <  2:846096/140‑1 (MQ=255)                  cTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCAcgcatcg                                                                                                                    <  1:651760/140‑1 (MQ=255)                           tGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCACGCATCGCATATAAAc                                                                                                           <  1:952447/140‑1 (MQ=255)                            ggCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGGCAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAAcc                                                                                                          <  2:776220/140‑1 (MQ=255)                            ggCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAAcc                                                                                                          <  2:1438569/140‑1 (MQ=255)                            ggCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAAcc                                                                                                          <  1:99395/140‑1 (MQ=255)                            ggCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATCGGTCAGTTCTTTTGTCTTTTCTGGATCGCCATGAAACAAAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAAcc                                                                                                          <  2:1287904/140‑1 (MQ=255)                             gCTTAGCGAACTTTGTCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCAcgcat                                                                                                                      <  2:869316/127‑1 (MQ=255)                             gCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCAcgcat                                                                                                                      >  1:869316/1‑127 (MQ=255)                             gCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCt                                                                                                         >  1:1079536/1‑140 (MQ=255)                                 aGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGtt                                                                                                     >  1:509153/1‑140 (MQ=255)                                 aGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGtt                                                                                                     >  1:662114/1‑140 (MQ=255)                                 aGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGtt                                                                                                     <  1:75574/140‑1 (MQ=255)                                  gCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCAcgcatcg                                                                                                                    >  2:243144/1‑124 (MQ=255)                                  gCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCAcgcatcg                                                                                                                    <  1:243144/124‑1 (MQ=255)                                  gCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTc                                                                                                    >  2:963819/1‑140 (MQ=255)                                   cGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAAcc                                                                                                          >  1:1095900/1‑133 (MQ=255)                                   cGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAAcc                                                                                                          <  2:1095900/133‑1 (MQ=255)                                   cGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCa                                                                                                   <  1:966309/140‑1 (MQ=255)                                       cTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGtt                                                                                                     <  2:1294054/134‑1 (MQ=255)                                       cTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGtt                                                                                                     >  1:1294054/1‑134 (MQ=255)                                           gCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACg                                                                                           >  2:895944/1‑140 (MQ=255)                                           gCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACg                                                                                           <  2:102370/140‑1 (MQ=255)                                            cccATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACg                                                                                           <  2:555064/139‑1 (MQ=255)                                            cccATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACg                                                                                           >  1:555064/1‑139 (MQ=255)                                            cccATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGc                                                                                          >  1:940134/1‑140 (MQ=255)                                            cccATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGc                                                                                          >  1:469975/1‑140 (MQ=255)                                                 tCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCa                                                                                                   <  2:1464397/126‑1 (MQ=255)                                                 tCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCa                                                                                                   >  1:1464397/1‑126 (MQ=255)                                                           cATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTgctgccgctgcc                                                                           <  1:895944/140‑1 (MQ=255)                                                            aTCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGctgccgctgccg                                                                          >  1:1225973/1‑140 (MQ=255)                                                             tCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGt                                                                         >  2:1324466/1‑140 (MQ=255)                                                             tCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGt                                                                         >  2:1269556/1‑140 (MQ=255)                                                              cTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGtt                                                                        >  1:264904/1‑140 (MQ=255)                                                               tGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGttt                                                                       >  1:1421380/1‑140 (MQ=255)                                                                  cAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTcc                                                                    >  2:691556/1‑139 (MQ=255)                                                                    gCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGtttt                                                                      <  2:998136/136‑1 (MQ=255)                                                                    gCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGtttt                                                                      >  1:998136/1‑136 (MQ=255)                                                                    gCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTgg                                                                  >  1:669487/1‑140 (MQ=255)                                                                      aaaGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGTCGCTGCCGTTTTCTGGGt                                                                <  2:497033/140‑1 (MQ=255)                                                                      aaaGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGt                                                                >  1:317549/1‑140 (MQ=255)                                                                        aGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGttt                                                              >  1:398114/1‑140 (MQ=255)                                                                          cGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGc                                                            <  2:662114/140‑1 (MQ=255)                                                                              aGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGaa                                                        <  1:1269556/140‑1 (MQ=255)                                                                                cTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCGCCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACt                                                      <  2:398114/140‑1 (MQ=255)                                                                                  tCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTg                                                    <  1:1083916/140‑1 (MQ=255)                                                                                    aTTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCt                                                  >  2:1208308/1‑140 (MQ=255)                                                                                                       ttttCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGc                               >  2:1093805/1‑140 (MQ=255)                                                                                                       ttttCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGc                               >  1:1181621/1‑140 (MQ=255)                                                                                                         ttCTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTa                             <  2:972879/140‑1 (MQ=255)                                                                                                           cTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTAcc                           <  2:469975/140‑1 (MQ=255)                                                                                                           cTGGATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTAcc                           <  2:1181621/140‑1 (MQ=255)                                                                                                              gATCGCCATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGa                        <  1:1093805/140‑1 (MQ=255)                                                                                                                   ccATGAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCAtt                   >  1:698010/1‑140 (MQ=255)                                                                                                                       gAAACAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATca               <  2:698010/140‑1 (MQ=255)                                                                                                                         aaCAGAGCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATcagc             <  2:1048341/140‑1 (MQ=255)                                                                                                                              agCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATca               <  2:901623/133‑1 (MQ=255)                                                                                                                              agCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATca               >  1:901623/1‑133 (MQ=255)                                                                                                                              agCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGccc        >  2:596602/1‑140 (MQ=255)                                                                                                                              agCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGccc        >  2:538122/1‑140 (MQ=255)                                                                                                                               gCCAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTCCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCa       <  1:683982/140‑1 (MQ=255)                                                                                                                                ccAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCAt      >  2:702586/1‑140 (MQ=255)                                                                                                                                 cAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATa     <  1:1033053/140‑1 (MQ=255)                                                                                                                                 cAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATa     <  1:1308792/140‑1 (MQ=255)                                                                                                                                 cAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATa     <  2:264904/140‑1 (MQ=255)                                                                                                                                 cAGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATa     <  1:702586/140‑1 (MQ=255)                                                                                                                                  aGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATcagca            <  1:1394630/132‑1 (MQ=255)                                                                                                                                  aGGCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATcagca            >  2:1394630/1‑132 (MQ=255)                                                                                                                                   ggCGCGCCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACa   >  2:351679/1‑140 (MQ=255)                                                                                                                                    gcgcgcCACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAg  <  2:1079536/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTGCTGCCGCTGCCGTTTTCTGGGTTTGCTGAACTTGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAG  >  NC_000913/2494080‑2494350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑