Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,507,863	A→G	V11A (GTG→GCG)	fryC ←	putative PTS enzyme IIC component FryC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,507,863	0	A	G	100.0%	262.9 / NA	76	V11A (GTG→GCG)	fryC	putative PTS enzyme IIC component FryC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/42);  total (34/42)

GGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCGTCCTGCAAAGTGAT  >  NC_000913/2507726‑2507996                                                                                                                                          |                                                                                                                                       ggATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGcgca                                                                                                                                      >  1:248781/1‑140 (MQ=255)            cccATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACt                                                                                                                           >  2:119762/1‑140 (MQ=255)            cccATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACt                                                                                                                           <  2:721944/140‑1 (MQ=255)             ccATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTg                                                                                                                          >  2:70249/1‑140 (MQ=255)                tAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGt                                                                                                                       <  1:935897/140‑1 (MQ=255)                      aGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑a                                                                                                                <  1:70249/140‑1 (MQ=255)                      aGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑a                                                                                                                <  2:248781/140‑1 (MQ=255)                      aGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑a                                                                                                                <  2:306832/140‑1 (MQ=255)                      aGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑a                                                                                                                <  2:830305/140‑1 (MQ=255)                      aGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑a                                                                                                                >  2:1223300/1‑140 (MQ=255)                       ggTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAGGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑aa                                                                                                               >  1:89343/1‑140 (MQ=255)                       ggTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑aa                                                                                                               <  2:87685/140‑1 (MQ=255)                        gTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AAt                                                                                                              >  1:863702/1‑140 (MQ=255)                         tCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATg                                                                                                             <  1:264134/140‑1 (MQ=255)                           ggCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGc                                                                                                           >  1:815388/1‑140 (MQ=255)                           ggCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGc                                                                                                           >  1:1368915/1‑140 (MQ=255)                           ggCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGc                                                                                                           >  2:598674/1‑140 (MQ=255)                           ggCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGc                                                                                                           >  2:511645/1‑140 (MQ=255)                           ggCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGc                                                                                                           >  2:1080233/1‑140 (MQ=255)                             cACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCa                                                                                                         >  2:1132557/1‑140 (MQ=255)                             cACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCa                                                                                                         <  1:252645/140‑1 (MQ=255)                              aCCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCAt                                                                                                        <  1:598674/140‑1 (MQ=255)                              aCCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCAt                                                                                                        <  1:782101/140‑1 (MQ=255)                              aCCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCAt                                                                                                        <  1:511645/140‑1 (MQ=255)                               ccATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAGGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATa                                                                                                       <  2:89343/140‑1 (MQ=255)                               ccATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACACCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATa                                                                                                       <  2:1368915/140‑1 (MQ=255)                                  tGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AAt                                                                                                              <  1:925842/130‑1 (MQ=255)                                  tGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AAt                                                                                                              >  2:925842/1‑130 (MQ=255)                                  tGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACa                                                                                                    >  2:186054/1‑140 (MQ=255)                                  tGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACa                                                                                                    >  2:14065/1‑140 (MQ=255)                                       gAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTAt                                                                                               <  2:1115809/140‑1 (MQ=255)                                         aaTCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATcc                                                                                             <  1:699312/140‑1 (MQ=255)                                          aTCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATccc                                                                                            <  2:815388/140‑1 (MQ=255)                                            cccTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTg                                                                                          <  1:14065/140‑1 (MQ=255)                                             ccTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGt                                                                                         >  2:99632/1‑140 (MQ=255)                                              cTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTTAAATGGCCATAACAATTATCCCTGt                                                                                         >  2:997102/1‑140 (MQ=255)                                              cTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTc                                                                                        <  2:1404797/140‑1 (MQ=255)                                                      acaTGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTa                                                                                <  2:863702/140‑1 (MQ=255)                                                      acaTGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTa                                                                                <  1:873325/140‑1 (MQ=255)                                                       caTGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTAc                                                                               >  1:41311/1‑140 (MQ=255)                                                         tgctgcGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACtg                                                                             <  1:1223300/140‑1 (MQ=255)                                                         tgctgcGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACtg                                                                             <  2:1439777/140‑1 (MQ=255)                                                              cGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTgttgtt                                                                        >  2:1241206/1‑140 (MQ=255)                                                              cGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTgttgtt                                                                        <  2:41311/140‑1 (MQ=255)                                                               gggAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTa                                                                                <  1:352945/131‑1 (MQ=255)                                                               gggAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTa                                                                                >  2:352945/1‑131 (MQ=255)                                                               gggAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTc                                                                       >  2:474272/1‑140 (MQ=255)                                                                  aGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTTAAATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCag                                                                     <  1:997102/140‑1 (MQ=255)                                                                      cACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCa                                                                >  2:1206985/1‑140 (MQ=255)                                                                              aaGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCAtctc                                                        >  2:676232/1‑140 (MQ=255)                                                                              aaGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCAtctc                                                        <  1:211290/140‑1 (MQ=255)                                                                              aaGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCAtctc                                                        >  2:211290/1‑140 (MQ=255)                                                                                    gTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGAt                                                  <  1:474272/140‑1 (MQ=255)                                                                                     ttttAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGAtt                                                 <  1:99632/140‑1 (MQ=255)                                                                                        tAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATctct                                              >  2:818621/1‑140 (MQ=255)                                                                                         agagGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCtt                                             >  2:1299799/1‑140 (MQ=255)                                                                                             gCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTtgat                                         <  2:1342805/140‑1 (MQ=255)                                                                                               cTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTtgatga                                       >  2:1053889/1‑140 (MQ=255)                                                                                               cTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTtgatga                                       >  2:806861/1‑140 (MQ=255)                                                                                                tGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTgatgat                                      <  2:542278/140‑1 (MQ=255)                                                                                                               gccgctgccgcACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGc                             >  2:370678/1‑134 (MQ=255)                                                                                                               gccgctgccgcACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGc                             >  2:75791/1‑134 (MQ=255)                                                                                                               gccgctgccgcACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGc                             <  1:370678/134‑1 (MQ=255)                                                                                                               gccgctgccgcACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGc                             <  1:75791/134‑1 (MQ=255)                                                                                                               gccgctgccgcACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGttttt                       >  2:90453/1‑140 (MQ=255)                                                                                                                     gccgcACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGACTTCTTTGATGATGCCCGCAGCGTTTTTAATTGc                 <  2:869968/140‑1 (MQ=255)                                                                                                                       cgcACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCGt               <  1:250681/140‑1 (MQ=255)                                                                                                                       cgcACCGGATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCGt               <  1:1206985/140‑1 (MQ=255)                                                                                                                              gATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCGTCCTGCaa        >  2:743694/1‑140 (MQ=255)                                                                                                                              gATGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCGTCCTGCaa        >  2:1240235/1‑140 (MQ=255)                                                                                                                               aTGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCGTCCTGCaaa       <  1:1240235/140‑1 (MQ=255)                                                                                                                               aTGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCGTCCTGCaaa       <  1:119762/140‑1 (MQ=255)                                                                                                                                tGCGCCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCGTCCTGCAAAg      >  1:653619/1‑140 (MQ=255)                                                                                                                                  cgcCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCGTCCTGCAAAGTg    <  1:1299799/140‑1 (MQ=255)                                                                                                                                   gcCAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCGTCCTGCAAAGTGa   <  2:678068/140‑1 (MQ=255)                                                                                                                                    ccAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCGTCCTGCAAAGTGAt  <  1:399391/140‑1 (MQ=255)                                                                                                                                    ccAGGCGCAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCGTCCTGCAAAGTGAt  >  2:475894/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTT‑AATGGCCATAACAATTATCCCTGTCGAATATTACTGTTGTTCAGAGGCAATCATCTCTTCGATTTCTTTGATGATGCCCGCAGCGTTTTTAATTGCGTCCTGCAAAGTGAT  >  NC_000913/2507726‑2507996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑