Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,547,294	A→G	T208A (ACC→GCC)	murP →	N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,547,294	0	A	G	100.0%	148.1 / NA	44	T208A (ACC→GCC)	murP	N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/13);  total (31/13)

GAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGACCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCTGCGCCCGCATTGAAGGCATGGTGCGCCGCTTTATGCCGGA  >  NC_000913/2547158‑2547427                                                                                                                                         |                                                                                                                                      gAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGgcca                                                                                                                                    <  1:1301838/140‑1 (MQ=255) gAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGgcca                                                                                                                                    <  2:1053479/140‑1 (MQ=255) gAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGgcca                                                                                                                                    <  1:245116/140‑1 (MQ=255)     gtgtTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCgg                                                                                                                                >  1:192414/1‑140 (MQ=255)        ttCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTta                                                                                                                             >  1:1365994/1‑140 (MQ=255)                  gTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGt                                                                                                                   >  2:1070782/1‑140 (MQ=255)                           cTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGAt                                                                                                          <  2:1365994/140‑1 (MQ=255)                            tttCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGAtt                                                                                                         >  2:1348028/1‑140 (MQ=255)                                 tGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCttt                                                                                                    >  1:1133350/1‑140 (MQ=255)                                 tGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCttt                                                                                                    >  1:1133351/1‑140 (MQ=255)                                  ggTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCTGGTTTTCACGATTTCTTTg                                                                                                   <  2:531526/140‑1 (MQ=255)                                    tGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCAc                                                                                                             >  1:1032518/1‑128 (MQ=255)                                    tGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCAc                                                                                                             <  2:1032518/128‑1 (MQ=255)                                    tGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGt                                                                                                 <  1:1348028/140‑1 (MQ=255)                                    tGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGt                                                                                                 <  2:1196940/140‑1 (MQ=255)                                     gATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTc                                                                                                <  1:1184748/140‑1 (MQ=255)                                      aTTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCt                                                                                               <  2:526738/140‑1 (MQ=255)                                        tCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGc                                                                                             >  2:89589/1‑140 (MQ=255)                                        tCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGc                                                                                             >  2:788595/1‑140 (MQ=255)                                        tCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGc                                                                                             <  2:248859/140‑1 (MQ=255)                                        tCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTAAAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGc                                                                                             >  1:580022/1‑140 (MQ=255)                                               ggCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGa                                                                                      >  2:375691/1‑140 (MQ=255)                                                     aaCGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCcgcg                                                                                <  2:192414/140‑1 (MQ=255)                                                        gccgccCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCgg                                                                             <  2:410663/140‑1 (MQ=255)                                                         ccgccCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTTCGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCcg                                                                                  <  2:605763/134‑1 (MQ=255)                                                         ccgccCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTTCGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCcg                                                                                  >  1:605763/1‑134 (MQ=255)                                                            cccAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAat                                                                         >  1:1331049/1‑140 (MQ=255)                                                            cccAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAat                                                                         >  1:26584/1‑140 (MQ=255)                                                                           gcACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTg                                                          >  2:452008/1‑140 (MQ=255)                                                                                    tAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCcgcg                                                 >  2:1202260/1‑140 (MQ=255)                                                                                        tGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTggg                                             >  2:663741/1‑140 (MQ=255)                                                                                           cgcAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCt                                          >  1:1442202/1‑140 (MQ=255)                                                                                           cgcAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCt                                          >  2:601946/1‑140 (MQ=255)                                                                                           cgcAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCt                                          >  2:1277522/1‑140 (MQ=255)                                                                                           cgcAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCt                                          >  1:83623/1‑140 (MQ=255)                                                                                           cgcAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGGCCACCGGTCACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCt                                          >  1:655003/1‑140 (MQ=255)                                                                                                    cgccgcGCTCTTTTTGCTCGGTTAAAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCTGCGCCCGCa                                 <  2:580022/140‑1 (MQ=255)                                                                                                                        gTTATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCTGCGCCCGCATTGAAGGCATGGTGcgccgc             >  1:347228/1‑140 (MQ=255)                                                                                                                         ttATAACCCCGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCTGCGCCCGCATTGAAGGCATGGTGCGCCGCt            <  2:347228/140‑1 (MQ=255)                                                                                                                                 ccGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCTGCGCCCGCATTGAAGGCATGGTGCGCCGCTTTATGCCg    >  2:1112578/1‑140 (MQ=255)                                                                                                                                 ccGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCTGCGCCCGCATTGAAGGCATGGTGCGCCGCTTTATGCCg    >  2:695725/1‑140 (MQ=255)                                                                                                                                 ccGCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCTGCGCCCGCATTGAAGGCATGGTGCGCCGCTTTATGCCg    >  1:1391517/1‑140 (MQ=255)                                                                                                                                   gCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCTGCGCCCGCATTGAAGGCATGGTGCGCCGCTTTATGCCGGa  >  1:431924/1‑140 (MQ=255)                                                                                                                                   gCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCTGCGCCCGCATTGAAGGCATGGTGCGCCGCTTTATGCCGGa  >  1:254643/1‑140 (MQ=255)                                                                                                                                   gCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCTGCGCCCGCATTGAAGGCATGGTGCGCCGCTTTATGCCGGa  >  2:631451/1‑140 (MQ=255)                                                                                                                                   gCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCTGCGCCCGCATTGAAGGCATGGTGCGCCGCTTTATGCCGGa  >  2:847399/1‑140 (MQ=255)                                                                                                                                   gCAGCGGCCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCTGCGCCCGCATTGAAGGCATGGTGCGCCGCTTTATGCCGGa  >  1:961951/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      GAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTCGGTTATAACCCCGCAGCGACCACCGGTTACTACGCCGGTTTTCACGATTTCTTTGGTCTGCCCATCGATCCGCGCGGCAATATTATCGGCGTGCTGATTGCCGCGTGGGCCTGCGCCCGCATTGAAGGCATGGTGCGCCGCTTTATGCCGGA  >  NC_000913/2547158‑2547427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑