Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,776,382	A→G	intergenic (+5/‑4)	yfjX → / → yfjY	CP4‑57 prophage; putative antirestriction protein/CP4‑57 prophage; RadC‑like JAB domain‑containing protein YfjY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,776,382	0	A	G	100.0%	293.5 / NA	85	intergenic (+5/‑4)	yfjX/yfjY	CP4‑57 prophage; putative antirestriction protein/CP4‑57 prophage; RadC‑like JAB domain‑containing protein YfjY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/40);  total (45/40)

CTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATACTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCATTCACCTCCACTCAGGCT  >  NC_000913/2776248‑2776517                                                                                                                                       |                                                                                                                                        cTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCat                                                                                                                                    >  1:943468/1‑140 (MQ=255) cTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCat                                                                                                                                    >  1:688573/1‑140 (MQ=255)    ccTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCatgat                                                                                                                                 >  2:12924/1‑140 (MQ=255)    ccTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACAGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCatgat                                                                                                                                 <  2:1167093/140‑1 (MQ=255)        aTTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGaa                                                                                                                             >  2:925907/1‑140 (MQ=255)        aTTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGaa                                                                                                                             <  2:320367/140‑1 (MQ=255)        aTTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGaa                                                                                                                             >  2:1195551/1‑140 (MQ=255)          tGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACa                                                                                                                           <  2:943468/140‑1 (MQ=255)          tGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACa                                                                                                                           <  1:12924/140‑1 (MQ=255)          tGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACa                                                                                                                           <  1:1035269/140‑1 (MQ=255)            aaTATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGt                                                                                                                         >  1:793077/1‑140 (MQ=255)                   ccATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAg                                                                                                                          >  2:946325/1‑132 (MQ=255)                   ccATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAg                                                                                                                          <  1:946325/132‑1 (MQ=255)                      tCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCatgatg                                                                                                                                >  1:1218549/1‑123 (MQ=255)                      tCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCatgatg                                                                                                                                <  2:1218549/123‑1 (MQ=255)                      tCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATccc                                                                                                               >  1:225233/1‑140 (MQ=255)                       cACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCca                                                                                                              >  1:668089/1‑140 (MQ=255)                       cACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCca                                                                                                              >  2:812206/1‑140 (MQ=255)                        aCGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCcac                                                                                                             >  2:740042/1‑140 (MQ=255)                        aCGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCcac                                                                                                             >  2:874492/1‑140 (MQ=255)                         cGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCcaca                                                                                                            <  1:874492/140‑1 (MQ=255)                           cctgcctCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGTGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGa                                                                                                          <  2:910650/140‑1 (MQ=255)                             tgcctCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGaca                                                                                                        >  1:1370027/1‑140 (MQ=255)                             tgcctCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGaca                                                                                                        <  1:1227267/140‑1 (MQ=255)                                ctCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCg                                                                                                     <  2:688573/140‑1 (MQ=255)                                   aCCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTa                                                                                                  <  1:925907/140‑1 (MQ=255)                                   aCCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTa                                                                                                  <  2:55108/140‑1 (MQ=255)                                   aCCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTa                                                                                                  <  1:740042/140‑1 (MQ=255)                                                         aGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCa                                                                            >  1:730816/1‑140 (MQ=255)                                                           cACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACg                                                                          >  2:206870/1‑140 (MQ=255)                                                                 tACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTaaaa                                                                    <  2:1370027/140‑1 (MQ=255)                                                                  aCCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAAc                                                                   <  2:793077/140‑1 (MQ=255)                                                                   ccGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAAcg                                                                  <  2:12387/140‑1 (MQ=255)                                                                   ccGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAAcg                                                                  <  2:730816/140‑1 (MQ=255)                                                                   ccGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAAcg                                                                  >  1:1181746/1‑140 (MQ=255)                                                                     gCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAAcgcg                                                                >  1:727445/1‑140 (MQ=255)                                                                     gCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAAcgcg                                                                <  2:294970/140‑1 (MQ=255)                                                                     gCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAAcgcg                                                                >  2:502528/1‑140 (MQ=255)                                                                       cTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGcc                                                              <  1:419058/140‑1 (MQ=255)                                                                        tGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCt                                                             >  1:679072/1‑140 (MQ=255)                                                                        tGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCt                                                             >  2:628179/1‑140 (MQ=255)                                                                        tGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCt                                                             >  2:859842/1‑140 (MQ=255)                                                                          cGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTa                                                           >  2:985664/1‑140 (MQ=255)                                                                                  cGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACgctgct                                                   <  2:727445/140‑1 (MQ=255)                                                                                  cGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACgctgct                                                   <  1:502528/140‑1 (MQ=255)                                                                                  cGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACgctgct                                                   <  2:679072/140‑1 (MQ=255)                                                                                  cGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACgctgct                                                   <  2:363555/140‑1 (MQ=255)                                                                                       tGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGAcc                                              >  1:1193094/1‑140 (MQ=255)                                                                                       tGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGAcc                                              >  2:306677/1‑140 (MQ=255)                                                                                         cggcaCCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGa                                            >  1:529093/3‑140 (MQ=255)                                                                                         cagcaCCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGa                                            >  1:692792/1‑140 (MQ=255)                                                                                            caCCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGacac                                         <  2:692792/140‑1 (MQ=255)                                                                                             aCCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACAcc                                        >  1:146625/1‑140 (MQ=255)                                                                                                 tGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACgctgct                                                   >  2:774913/1‑125 (MQ=255)                                                                                                 tGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACgctgct                                                   <  1:774913/125‑1 (MQ=255)                                                                                                 tGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTgcg                                    >  1:1185372/1‑140 (MQ=255)                                                                                                 tGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTgcg                                    >  1:1226652/1‑140 (MQ=255)                                                                                                 tGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTgcg                                    >  2:454028/1‑140 (MQ=255)                                                                                                   aaTCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTgcgca                                  >  2:167953/1‑139 (MQ=255)                                                                                                   aaTCCAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGcgcg                                  >  1:326083/1‑140 (MQ=255)                                                                                                      ccAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGaaa                               <  1:628179/140‑1 (MQ=255)                                                                                                      ccAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGaaa                               >  2:92441/1‑140 (MQ=255)                                                                                                      ccAGCGCCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGaaa                               <  2:529093/140‑1 (MQ=255)                                                                                                         gcgcCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAAc                              >  1:710091/1‑138 (MQ=255)                                                                                                         gcgcCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAAc                              <  2:710091/138‑1 (MQ=255)                                                                                                         gcgcCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAg                            >  1:120326/1‑140 (MQ=255)                                                                                                           gcCATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGc                          <  1:313287/140‑1 (MQ=255)                                                                                                             cATTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGt                        >  2:810223/1‑140 (MQ=255)                                                                                                              aTTATGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTg                       <  1:859842/140‑1 (MQ=255)                                                                                                                  tGCGCATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCAt                   <  1:206870/140‑1 (MQ=255)                                                                                                                   gcgcATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCAtt                  <  2:668089/140‑1 (MQ=255)                                                                                                                   gcgcATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCAtt                  <  1:92441/140‑1 (MQ=255)                                                                                                                   gcgcATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCAtt                  <  1:306677/140‑1 (MQ=255)                                                                                                                   gcgcATTATTGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCAAAACAGGCGTGGCAtt                  <  1:167953/140‑1 (MQ=255)                                                                                                                       attattGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCATTCAcc              <  2:1083341/140‑1 (MQ=255)                                                                                                                       attattGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCATTCAcc              >  2:1253293/1‑140 (MQ=255)                                                                                                                       attattGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCATTCAcc              >  2:574499/1‑140 (MQ=255)                                                                                                                         tattGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCATTCAcc              <  2:720333/138‑1 (MQ=255)                                                                                                                         tattGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCATTCAcc              >  1:720333/1‑138 (MQ=255)                                                                                                                           ttGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCATTCACCTCCa          >  1:5618/1‑140 (MQ=255)                                                                                                                            tGATTAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCATTCACCTCCAc         >  1:299817/1‑140 (MQ=255)                                                                                                                                tAAGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCATTCACCTCCACTCAg     >  1:806316/1‑140 (MQ=255)                                                                                                                                 aaGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCATTCACCTCCACTCAgg    <  2:717372/140‑1 (MQ=255)                                                                                                                                 aaGGATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCATTCACCTCCACTCAgg    <  2:1181746/140‑1 (MQ=255)                                                                                                                                   ggATGCTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCATTCACCTCCACTCAGgct  >  1:670059/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        CTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGACGGAGCACTATTACCGCCTGCGGGATTACGCTCTGCAGCACCCTGAATCCAGCGCCATTATGCGCATTATTGATTAAGGATACTCATGATGGAACAGTCACTCATCCCACAGACACCGGTACTTCCACTGACCGCACAACGCACGGTAAAACGCGCCTTAACGCTGCTTGACCGACACCTGCGCGAAACAGGCGTGGCATTCACCTCCACTCAGGCT  >  NC_000913/2776248‑2776517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑