Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	155,950	C→A	S84S (TCG→TCT)	yadV ←	putative fimbrial chaperone YadV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	155,950	0	C	A	100.0%	284.4 / NA	80	S84S (TCG→TCT)	yadV	putative fimbrial chaperone YadV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (40/40);  total (40/40)

GCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGAT  >  NC_000913/155816‑156086                                                                                                                                       |                                                                                                                                         gcgACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATAc                                                                                                                                     >  2:885344/1‑140 (MQ=255)  cgACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACt                                                                                                                                    >  1:1377835/1‑140 (MQ=255)                   ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTaaa                                                                                                                   >  2:1441076/1‑140 (MQ=255)                    gTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTaaaa                                                                                                                  <  2:1377835/140‑1 (MQ=255)                      tttGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAgg                                                                                                                <  2:343927/140‑1 (MQ=255)                        tggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGa                                                                                                              <  2:1252680/140‑1 (MQ=255)                           tggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTg                                                                                                           <  2:1289628/140‑1 (MQ=255)                                         cGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGc                                                                                             <  2:1464111/140‑1 (MQ=255)                                            tAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCa                                                                                          >  1:648616/1‑140 (MQ=255)                                            tAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCa                                                                                          >  2:553684/1‑140 (MQ=255)                                             aaaCCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAg                                                                                         >  1:594383/1‑140 (MQ=255)                                               aCCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCg                                                                                       <  1:597357/140‑1 (MQ=255)                                                   gAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGt                                                                                   >  1:1108401/1‑140 (MQ=255)                                                    aaCACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTc                                                                                  >  2:792074/1‑140 (MQ=255)                                                    aaCACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTc                                                                                  >  2:808418/1‑140 (MQ=255)                                                     acacGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCa                                                                                 <  2:594383/140‑1 (MQ=255)                                                         gCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTAATCGc                                                                             <  1:32949/140‑1 (MQ=255)                                                          ctctctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATTGcc                                                                            <  1:792074/140‑1 (MQ=255)                                                           tctctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCa                                                                           >  1:886588/1‑140 (MQ=255)                                                           tctctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCa                                                                           <  1:263901/140‑1 (MQ=255)                                                               tctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTAt                                                                       <  2:389460/140‑1 (MQ=255)                                                                   tCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCAt                                                                                >  2:685359/1‑127 (MQ=255)                                                                   tCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCAt                                                                                <  1:685359/127‑1 (MQ=255)                                                                   tCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCa                                                                           <  1:728021/132‑1 (MQ=255)                                                                   tCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCa                                                                           >  2:728021/1‑132 (MQ=255)                                                                    cTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAAc                                                                  <  2:648616/140‑1 (MQ=255)                                                                     tttAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAAcc                                                                 <  1:54746/140‑1 (MQ=255)                                                                       tAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCaa                                                               >  2:137011/1‑140 (MQ=255)                                                                         ggCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAAct                                                             >  2:1063461/1‑140 (MQ=255)                                                                           cAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAActct                                                           >  1:790684/1‑140 (MQ=255)                                                                             gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTgg                                                         >  1:859713/1‑140 (MQ=255)                                                                             gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTgg                                                         >  1:652140/1‑140 (MQ=255)                                                                             gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTgg                                                         >  1:555944/1‑140 (MQ=255)                                                                             gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTgg                                                         >  1:22931/1‑140 (MQ=255)                                                                             gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTgg                                                         >  1:1177142/1‑140 (MQ=255)                                                                              tGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGa                                                        >  2:1205785/1‑140 (MQ=255)                                                                              tGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGa                                                        <  2:111635/140‑1 (MQ=255)                                                                               gAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGAc                                                       >  1:971764/1‑140 (MQ=255)                                                                               gAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGAc                                                       <  2:859713/140‑1 (MQ=255)                                                                                   tGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGc                                                   <  2:886588/140‑1 (MQ=255)                                                                                    gctggctgTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCa                                                  <  2:555944/140‑1 (MQ=255)                                                                                    gctggctgTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCa                                                  <  2:22931/140‑1 (MQ=255)                                                                                        gctgTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCGATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACgg                                              >  1:381481/1‑140 (MQ=255)                                                                                        gctgTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACgg                                              >  2:781357/1‑140 (MQ=255)                                                                                           gtgtACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGtt                                           <  2:1145954/140‑1 (MQ=255)                                                                                            tgtACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTa                                          >  1:1375671/1‑140 (MQ=255)                                                                                            tgtACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTa                                          >  1:565853/1‑140 (MQ=255)                                                                                             gtACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTAt                                         <  2:1337124/140‑1 (MQ=255)                                                                                                  ttAATTTGATTGTTTGCCCACGTTTGGTATCAATACGAGATACAGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCt                                    <  2:1343720/140‑1 (MQ=255)                                                                                                  ttAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCt                                    >  1:450721/1‑140 (MQ=255)                                                                                                  ttAATTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCt                                    >  1:95599/1‑140 (MQ=255)                                                                                                     aTTTGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTa                                 <  1:1441076/140‑1 (MQ=255)                                                                                                      tttGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTAt                                >  2:266090/1‑140 (MQ=255)                                                                                                      tttGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTAt                                >  2:592259/1‑140 (MQ=255)                                                                                                        tGATTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTAttt                              >  1:466898/1‑140 (MQ=255)                                                                                                          aTTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTc                            >  1:1401752/1‑140 (MQ=255)                                                                                                          aTTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTc                            <  2:1401752/140‑1 (MQ=255)                                                                                                          aTTGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTc                            <  2:790684/140‑1 (MQ=255)                                                                                                           ttGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTa                                 <  1:887894/134‑1 (MQ=255)                                                                                                           ttGTTTGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTa                                 >  2:887894/1‑134 (MQ=255)                                                                                                               ttGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGac                       >  2:934259/1‑140 (MQ=255)                                                                                                                tGCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGacg                      >  1:273003/1‑140 (MQ=255)                                                                                                                 gCCCACGTTTGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGacgt                     <  2:273003/140‑1 (MQ=255)                                                                                                                        tttGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGa              >  1:4257/1‑140 (MQ=255)                                                                                                                         ttGGCATCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGac             <  1:781357/140‑1 (MQ=255)                                                                                                                               tCAATACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACtttt       <  1:563465/140‑1 (MQ=255)                                                                                                                                 aaTACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACtttttt     >  2:39455/1‑140 (MQ=255)                                                                                                                                 aaTACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACtttttt     >  2:1044191/1‑140 (MQ=255)                                                                                                                                 aaTACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACtttttt     >  2:1365254/1‑140 (MQ=255)                                                                                                                                   tACGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGa   >  2:143201/1‑140 (MQ=255)                                                                                                                                    aCGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGAt  <  2:104436/140‑1 (MQ=255)                                                                                                                                    aCGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGAt  <  2:1108401/140‑1 (MQ=255)                                                                                                                                    aCGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGAt  <  1:1260057/140‑1 (MQ=255)                                                                                                                                    aCGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGAt  <  1:885344/140‑1 (MQ=255)                                                                                                                                    aCGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGAt  <  1:137011/140‑1 (MQ=255)                                                                                                                                    aCGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGAt  <  1:808418/140‑1 (MQ=255)                                                                                                                                    aCGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGAt  <  2:1375671/140‑1 (MQ=255)                                                                                                                                    aCGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGAt  <  2:652140/140‑1 (MQ=255)                                                                                                                                    aCGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGAt  <  2:381481/140‑1 (MQ=255)                                                                                                                                    aCGAGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGAt  <  2:4257/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGAT  >  NC_000913/155816‑156086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑