Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,078,631	G→A	R77C (CGC→TGC)	cmtA ←	mannitol‑specific PTS enzyme IICB component CmtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,078,631	0	G	A	100.0%	352.5 / NA	99	R77C (CGC→TGC)	cmtA	mannitol‑specific PTS enzyme IICB component CmtA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (52/47);  total (52/47)

CCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCGTTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAAC  >  NC_000913/3078502‑3078765                                                                                                                                  |                                                                                                                                       ccAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTAccgccg                                                                                                                              <  2:180023/140‑1 (MQ=255)   aGTGCCTTATCAACATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGAc                                                                                                                            <  1:423356/140‑1 (MQ=255)    gTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGAcc                                                                                                                           <  2:914038/140‑1 (MQ=255)    gTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGAcc                                                                                                                           >  2:18520/1‑140 (MQ=255)      gCCTTATCGACATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTAccg                                                                                                                                 <  2:96216/132‑1 (MQ=255)      gCCTTATCGACATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTAccg                                                                                                                                 >  1:96216/1‑132 (MQ=255)       ccTTATCGACATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGa                                                                                                                        >  2:562783/1‑140 (MQ=255)         ttATCGACATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATg                                                                                                                      <  2:1396374/140‑1 (MQ=255)          tATCGACATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGa                                                                                                                     >  2:689160/1‑140 (MQ=255)          tATCGACATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGa                                                                                                                     <  1:562783/140‑1 (MQ=255)            tCGACATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGacc                                                                                                                   <  2:372086/140‑1 (MQ=255)            tCGACATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGacc                                                                                                                   <  1:18520/140‑1 (MQ=255)               aCATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGa                                                                                                                        >  2:4733/1‑132 (MQ=255)               aCATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGa                                                                                                                        <  1:4733/132‑1 (MQ=255)                      ttATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGaa                                                                                                         <  2:1194537/140‑1 (MQ=255)                      ttATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGaa                                                                                                         >  2:689483/1‑140 (MQ=255)                               aCCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAatcatc                                                                                                >  2:618067/1‑140 (MQ=255)                               aCCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAatcatc                                                                                                >  1:31720/1‑140 (MQ=255)                                 ccACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCAc                                                                                              <  1:1151768/140‑1 (MQ=255)                                 ccACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCAc                                                                                              <  2:31720/140‑1 (MQ=255)                                 ccACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCAc                                                                                              <  1:704518/140‑1 (MQ=255)                                    ccGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACggg                                                                                           <  1:1093544/140‑1 (MQ=255)                                    ccGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCACCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACggg                                                                                           <  2:1183501/140‑1 (MQ=255)                                     cGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGc                                                                                          >  1:758219/1‑140 (MQ=255)                                       aGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCaa                                                                                        >  2:695134/1‑140 (MQ=255)                                       aGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCaa                                                                                        <  1:434308/140‑1 (MQ=255)                                       aGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCaa                                                                                        >  1:852675/1‑140 (MQ=255)                                       aGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCaa                                                                                        <  1:618067/140‑1 (MQ=255)                                         cGGCCCCATGATCATTGAGCCAGGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCG‑CCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAAtcatca                                                                                               <  1:239448/130‑1 (MQ=255)                                         cGGCCCCATGATCATTGAGCCAGGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCG‑CCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAAtcatca                                                                                               >  2:239448/1‑130 (MQ=255)                                           gCCCCATGATCATTGAGCCAAGGAACATCGGGCTCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCAc                                                                                              <  2:1367023/130‑1 (MQ=255)                                           gCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGTGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCaataaa                                                                                    <  2:1403977/140‑1 (MQ=255)                                           gCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCAc                                                                                              >  1:1367023/1‑130 (MQ=255)                                           gCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCaataaa                                                                                    <  1:214627/140‑1 (MQ=255)                                           gCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCaataaa                                                                                    >  1:934144/1‑140 (MQ=255)                                           gCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCaataaa                                                                                    >  2:339893/1‑140 (MQ=255)                                           gCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGAGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACggg                                                                                           <  1:1023314/133‑1 (MQ=255)                                           gCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGAGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACggg                                                                                           >  2:1023314/1‑133 (MQ=255)                                                    tCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAatc                                                                           <  2:941498/140‑1 (MQ=255)                                                          aGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCggg                                                                     >  2:427946/1‑140 (MQ=255)                                                               aGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGAc                                                                >  1:1350930/1‑140 (MQ=255)                                                               aGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGAc                                                                >  1:758760/1‑140 (MQ=255)                                                               aGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGAc                                                                >  2:1123245/1‑140 (MQ=255)                                                                    cATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAAttt                                                           >  2:465366/1‑140 (MQ=255)                                                                      tCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTg                                                         >  2:13174/1‑140 (MQ=255)                                                                        gggATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGc                                                       <  2:400216/140‑1 (MQ=255)                                                                         ggATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCg                                                      >  1:1460721/1‑140 (MQ=255)                                                                            tctcTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGaaa                                                   >  1:341470/1‑140 (MQ=255)                                                                              tctGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCACATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATg                                                 >  2:1368046/1‑140 (MQ=255)                                                                               ctGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGt                                                >  2:1104487/1‑140 (MQ=255)                                                                                   gcCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGt                                            >  1:119976/1‑140 (MQ=255)                                                                                       aCGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAgg                                        >  2:491215/1‑140 (MQ=255)                                                                                       aCGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAgg                                        >  1:577116/1‑140 (MQ=255)                                                                                               acCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAAc                                <  2:119976/140‑1 (MQ=255)                                                                                               acCAATAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAAc                                >  1:944477/1‑140 (MQ=255)                                                                                                  aaTAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCgg                             <  2:1460721/140‑1 (MQ=255)                                                                                                  aaTAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCgg                             <  2:277990/140‑1 (MQ=255)                                                                                                  aaTAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCgg                             <  1:427946/140‑1 (MQ=255)                                                                                                  aaTAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCgg                             <  1:1104487/140‑1 (MQ=255)                                                                                                  aaTAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCgg                             <  1:695134/140‑1 (MQ=255)                                                                                                   aTAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGt                            >  2:534686/1‑140 (MQ=255)                                                                                                   aTAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGt                            <  1:465366/140‑1 (MQ=255)                                                                                                    tAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCgg                             <  2:330656/138‑1 (MQ=255)                                                                                                    tAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCgg                             >  1:330656/1‑138 (MQ=255)                                                                                                    tAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGt                            >  2:1261765/1‑139 (MQ=255)                                                                                                    tAGTACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGt                            <  1:1261765/139‑1 (MQ=255)                                                                                                       tACCTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGa                        <  2:758219/140‑1 (MQ=255)                                                                                                         ccTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCgg                             >  1:1275649/1‑133 (MQ=255)                                                                                                         ccTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCgg                             <  2:1275649/133‑1 (MQ=255)                                                                                                         ccTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGaat                      >  2:1106025/1‑140 (MQ=255)                                                                                                         ccTATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGaat                      >  2:79437/1‑140 (MQ=255)                                                                                                           tATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGaataa                    >  1:1234591/1‑140 (MQ=255)                                                                                                           tATTCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGaataa                    >  2:638044/1‑140 (MQ=255)                                                                                                              tCCGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGaataaata                 >  1:490215/1‑140 (MQ=255)                                                                                                                cGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAataaataa                >  1:534014/1‑139 (MQ=255)                                                                                                                cGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAataaataa                <  2:534014/139‑1 (MQ=255)                                                                                                                cGCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAAc               >  2:188776/1‑140 (MQ=255)                                                                                                                 gCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCgg                             >  1:1451943/1‑125 (MQ=255)                                                                                                                 gCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCgg                             <  2:1451943/125‑1 (MQ=255)                                                                                                                 gCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACg              >  1:679650/1‑140 (MQ=255)                                                                                                                 gCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACg              <  2:934144/140‑1 (MQ=255)                                                                                                                 gCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACg              <  2:577116/140‑1 (MQ=255)                                                                                                                 gCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACg              >  2:526538/1‑140 (MQ=255)                                                                                                                 gCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACg              <  2:341470/140‑1 (MQ=255)                                                                                                                 gCCCATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTAGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACg              >  2:335767/1‑140 (MQ=255)                                                                                                                  cccATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGa                        >  2:631958/1‑129 (MQ=255)                                                                                                                  cccATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGa                        <  1:631958/129‑1 (MQ=255)                                                                                                                  cccATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGc             >  2:1193150/1‑140 (MQ=255)                                                                                                                  cccATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGc             <  2:490215/140‑1 (MQ=255)                                                                                                                  cccATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGc             >  1:1425451/1‑140 (MQ=255)                                                                                                                  cccATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGc             <  2:944477/140‑1 (MQ=255)                                                                                                                  cccATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGc             <  1:491215/140‑1 (MQ=255)                                                                                                                  cccATGACCGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGc             <  1:689160/140‑1 (MQ=255)                                                                                                                         ccGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGtaata      <  2:679650/140‑1 (MQ=255)                                                                                                                         ccGCCCCGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGtaata      <  2:852675/140‑1 (MQ=255)                                                                                                                            ccccGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATaaaa   >  2:534419/1‑140 (MQ=255)                                                                                                                            ccccGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATaaaa   >  1:670744/1‑140 (MQ=255)                                                                                                                            ccccGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATaaaa   >  1:949106/1‑140 (MQ=255)                                                                                                                             cccGCATTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAAc  >  2:326528/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                       CCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGCCCCATGATCATTGAGCCAAGGAACATCGGGATCTCTGCGCCAACGATCACACCAATAGTACCTATTCCGCCCATGACCGCCCCGCGTTTACCGCCGACCAGATGACCACCTGTAGAACCAATCATCACGGGCAATAAATAGGTAATCATCGGGCCGACAATTTTGGCGAAATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAAC  >  NC_000913/3078502‑3078765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑