Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,112,839	T→C	pseudogene (82/867 nt)	yghF ←	putative type II secretion system C‑type protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,112,839	0	T	C	100.0%	292.6 / NA	84	pseudogene (82/867 nt)	yghF	putative type II secretion system C‑type protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/44);  total (40/44)

GGGTTGTTTTACCTGCGCGGCGACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGTATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTG  >  NC_000913/3112705‑3112974                                                                                                                                       |                                                                                                                                        gggTTGTTTTACCTGCGCGGCGACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATAtt                                                                                                                                    >  2:1322178/1‑140 (MQ=255)      gttTTACCTGCGCGGCGACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCa                                                                                                                               <  1:1371752/140‑1 (MQ=255)        tttACCTGCGCGGCGACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGa                                                                                                                             <  2:1241077/140‑1 (MQ=255)        tttACCTGCGCGGCGACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGa                                                                                                                             <  2:90636/140‑1 (MQ=255)        tttACCTGCGCGGCGACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGa                                                                                                                             <  1:1186985/140‑1 (MQ=255)        tttACCTGCGCGGCGACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGa                                                                                                                             <  2:188432/140‑1 (MQ=255)          tACCTGCGCGGCGACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGaaa                                                                                                                           <  2:786731/140‑1 (MQ=255)           aCCTGCGCGGCGACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGaaaa                                                                                                                          <  1:1220338/140‑1 (MQ=255)              tGCGCGGCGACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGga                                                                                                                       >  2:875816/1‑140 (MQ=255)               gcgcGGCGACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGaaa                                                                                                                           <  1:1454867/135‑1 (MQ=255)               gcgcGGCGACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGaaa                                                                                                                           >  2:1454867/1‑135 (MQ=255)                 gcggcgACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCg                                                                                                                                 <  1:588450/127‑1 (MQ=255)                 gcggcgACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCg                                                                                                                                 >  2:588450/1‑127 (MQ=255)                 gcggcgACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAgg                                                                                                                        >  1:1258819/1‑136 (MQ=255)                 gcggcgACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAgg                                                                                                                        <  2:1258819/136‑1 (MQ=255)                 gcggcgACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGat                                                                                                                    >  2:1445871/1‑140 (MQ=255)                 gcggcgACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGat                                                                                                                    >  1:1438368/1‑140 (MQ=255)                 gcggcgACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGat                                                                                                                    >  2:1171484/1‑140 (MQ=255)                 gcggcgACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGat                                                                                                                    >  1:602486/1‑140 (MQ=255)                  cggcgACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGata                                                                                                                   <  2:1438368/140‑1 (MQ=255)                  cggcgACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGata                                                                                                                   >  2:1248217/1‑140 (MQ=255)                  cggcgACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGata                                                                                                                   <  2:760717/140‑1 (MQ=255)                    gcgACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGAtata                                                                                                                 <  1:1445871/140‑1 (MQ=255)                      gACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTTACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAgc                                                                                                               >  2:73047/1‑140 (MQ=255)                             tGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCagag                                                                                                        >  1:1280375/1‑140 (MQ=255)                                     gCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGaa                                                                                                    <  2:969627/136‑1 (MQ=255)                                     gCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGaa                                                                                                    >  1:969627/1‑136 (MQ=255)                                     gCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATgcg                                                                                                >  1:390338/1‑140 (MQ=255)                                     gCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATgcg                                                                                                >  2:1144701/1‑140 (MQ=255)                                     gCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATgcg                                                                                                >  1:677136/1‑140 (MQ=255)                                         aaCCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCAt                                                                                            >  1:583778/1‑140 (MQ=255)                                            cAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCAtttt                                                                                         <  1:1171484/140‑1 (MQ=255)                                             aGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCAttttt                                                                                        <  2:876771/140‑1 (MQ=255)                                              gTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTg                                                                                       <  2:677136/140‑1 (MQ=255)                                                 ttGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAg                                                                                    <  1:1312493/140‑1 (MQ=255)                                                       gctgATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCAttttt                                                                                        >  2:1255012/1‑130 (MQ=255)                                                       gctgATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCAttttt                                                                                        <  1:1255012/130‑1 (MQ=255)                                                           aTTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAag                                                                          <  1:1248217/140‑1 (MQ=255)                                                                ttGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAgcagca                                                                     <  2:140354/140‑1 (MQ=255)                                                                ttGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAgcagca                                                                     <  2:647733/140‑1 (MQ=255)                                                                ttGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAgcagca                                                                     <  1:890640/140‑1 (MQ=255)                                                                 tGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAg                                                                                    >  1:921193/1‑124 (MQ=255)                                                                 tGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAg                                                                                    <  2:921193/124‑1 (MQ=255)                                                                 tGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCAt                                                                    >  2:418061/1‑140 (MQ=255)                                                                   cACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCa                                                                  >  1:1361194/1‑140 (MQ=255)                                                                    aCGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAg                                                                 <  1:1322178/140‑1 (MQ=255)                                                                     cGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAg                                                                 >  2:1128220/1‑139 (MQ=255)                                                                     cGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAg                                                                 <  1:1128220/139‑1 (MQ=255)                                                                     cGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGc                                                                >  1:877674/1‑140 (MQ=255)                                                                      gTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGcc                                                               >  2:802765/1‑140 (MQ=255)                                                                      gTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGcc                                                               >  1:1167950/1‑140 (MQ=255)                                                                        cATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCAGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAg                                                             >  2:1314286/1‑140 (MQ=255)                                                                          tttttATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAag                                                                          <  2:352371/125‑1 (MQ=255)                                                                          tttttATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAag                                                                          >  1:352371/1‑125 (MQ=255)                                                                                               tCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCg                                      <  1:908610/140‑1 (MQ=255)                                                                                                 cacaCGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCa                                    >  2:1222932/1‑140 (MQ=255)                                                                                                 cacaCGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCa                                    >  1:1356626/1‑140 (MQ=255)                                                                                                       gCTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGct                              >  1:529860/1‑140 (MQ=255)                                                                                                        cTCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGctc                             >  1:588838/1‑140 (MQ=255)                                                                                                         tCGGTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGctct                            >  1:133539/1‑140 (MQ=255)                                                                                                            gTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTg                         <  2:1361194/140‑1 (MQ=255)                                                                                                            gTTTATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTg                         <  2:529860/140‑1 (MQ=255)                                                                                                             tttATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGt                        <  2:602486/140‑1 (MQ=255)                                                                                                              ttATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTc                       <  2:141687/140‑1 (MQ=255)                                                                                                              ttATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTc                       <  1:418061/140‑1 (MQ=255)                                                                                                              ttATTCACCGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCAGCGTGCAATTTTTCGCAGATGCTCTTTGTc                       <  2:1167950/140‑1 (MQ=255)                                                                                                                     ccGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTa                >  1:442570/1‑140 (MQ=255)                                                                                                                      cGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGGAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTaa               >  2:1024672/1‑140 (MQ=255)                                                                                                                      cGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTg                         <  1:224198/130‑1 (MQ=255)                                                                                                                      cGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTg                         >  1:44199/1‑130 (MQ=255)                                                                                                                      cGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTg                         >  2:224198/1‑130 (MQ=255)                                                                                                                      cGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTg                         <  2:44199/130‑1 (MQ=255)                                                                                                                      cGGTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTaa               >  1:1403794/1‑140 (MQ=255)                                                                                                                       ggTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGt                        >  2:463556/1‑130 (MQ=255)                                                                                                                       ggTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGt                        <  1:463556/130‑1 (MQ=255)                                                                                                                       ggTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTc                       >  2:1415094/1‑131 (MQ=255)                                                                                                                       ggTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTc                       <  1:1415094/131‑1 (MQ=255)                                                                                                                       ggTTGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAag              >  2:1003072/1‑140 (MQ=255)                                                                                                                          tGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAgaga           <  2:1403794/140‑1 (MQ=255)                                                                                                                          tGAGAAACCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAgaga           <  1:73047/140‑1 (MQ=255)                                                                                                                              aaaCCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGt       <  1:1024672/140‑1 (MQ=255)                                                                                                                                aCCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGtct     <  2:133539/140‑1 (MQ=255)                                                                                                                                aCCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGtct     <  1:1222932/140‑1 (MQ=255)                                                                                                                                aCCGCCGCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGtct     <  1:648643/140‑1 (MQ=255)                                                                                                                                   gccgCATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTg  >  1:1316973/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        GGGTTGTTTTACCTGCGCGGCGACAGGCTGATATTTGCCAAACCAGTTTTGCTGGCTGATTAATTGCACGTCATTTTTATCGAACGTTTTTGCATCCACACGGCTCGGTTTATTCACCGGTTGAGAAACCGCCGTATATTCCGCAGAAAAGGAGATATAGCGCCAGAGTGAATGCGCCATTTTTGCAGAAATAATAAGCAGCATCAGCCAGAACATCCCGCGTGCAATTTTTCGCAGATGCTCTTTGTCTGTATTAAGAGATTGTCTCTG  >  NC_000913/3112705‑3112974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑