BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,184,007	T→C	T154A (ACT→GCT)	ribB ←	3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,184,007	0	T	C	100.0%	361.2 / NA	104	T154A (ACT→GCT)	ribB	3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (58/46); total (58/46)

Only 100 of 104 total aligned reads displayed.

GAGCGCCATATTGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACA  >  NC_000913/3183870‑3184143

gAGCGCCATATTGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCAt                                                                                                                                        >  1:186784/1‑140 (MQ=255)

 aGCGCCATATTGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATg                                                                                                                                       >  1:1043587/1‑140 (MQ=255)

   cgcCATATTGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGAc                                                                                                                                     <  2:765919/140‑1 (MQ=255)

   cgcCATATTGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGAc                                                                                                                                     >  1:654427/1‑140 (MQ=255)

   cgcCATATTGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGAc                                                                                                                                     <  1:85879/140‑1 (MQ=255)

      cATATTGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGc                                                                                                                                  >  2:121546/1‑140 (MQ=255)

      cATATTGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGc                                                                                                                                  >  2:438249/1‑140 (MQ=255)

        tatTGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCa                                                                                                                                <  2:1353346/140‑1 (MQ=255)

        tatTGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCa                                                                                                                                <  2:911761/140‑1 (MQ=255)

        tatTGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCa                                                                                                                                <  1:684831/140‑1 (MQ=255)

          ttGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCAcg                                                                                                                              <  2:186784/140‑1 (MQ=255)

          ttGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCAcg                                                                                                                              <  2:654427/140‑1 (MQ=255)

          ttGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCAcg                                                                                                                              <  2:1059908/140‑1 (MQ=255)

          ttGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCAcg                                                                                                                              <  2:750594/140‑1 (MQ=255)

           tgtgTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCAcgc                                                                                                                             <  1:904201/140‑1 (MQ=255)

               tttATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCAcg                                                                                                                              >  2:571954/1‑135 (MQ=255)

               tttATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCAcg                                                                                                                              <  1:571954/135‑1 (MQ=255)

                  aTTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTa                                                                                                                      <  1:215648/140‑1 (MQ=255)

                  aTTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTa                                                                                                                      >  2:215648/1‑140 (MQ=255)

                  aTTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTa                                                                                                                      <  1:576769/140‑1 (MQ=255)

                  aTTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTa                                                                                                                      <  2:608104/140‑1 (MQ=255)

                  aTTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTa                                                                                                                      >  1:989800/1‑140 (MQ=255)

                       cAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTAcacca                                                                                                                 >  2:577311/1‑140 (MQ=255)

                         aaCTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACaccacc                                                                                                               >  1:415053/1‑140 (MQ=255)

                                tACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGa                                                                                                        >  1:549620/1‑140 (MQ=255)

                                     tctGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAgcg                                                                                                     >  1:146603/1‑138 (MQ=255)

                                     tctGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAgcg                                                                                                     <  2:146603/138‑1 (MQ=255)

                                     tctGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGcgcg                                                                                                   >  1:1000157/1‑140 (MQ=255)

                                     tctGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGcgcg                                                                                                   <  1:165175/140‑1 (MQ=255)

                                      ctGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGa                                                                                                  >  2:1411422/1‑140 (MQ=255)

                                      ctGGTGCACGCGCCATCGTGCAATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGa                                                                                                  >  1:1324625/1‑140 (MQ=255)

                                          tGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTg                                                                                              >  1:391763/1‑140 (MQ=255)

                                          tGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTg                                                                                              >  2:450030/1‑140 (MQ=255)

                                                 gccatcgtgccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGcgcg                                                                                                   >  2:1413811/1‑128 (MQ=255)

                                                 gccatcgtgccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGcgcg                                                                                                   <  1:1413811/128‑1 (MQ=255)

                                                 gccatcgtgccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAAc                                                                                       <  1:446861/140‑1 (MQ=255)

                                                 gccatcgtgccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAAc                                                                                       <  2:1043587/140‑1 (MQ=255)

                                                 gccatcgtgccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAAc                                                                                       >  2:636585/1‑140 (MQ=255)

                                                 gccatcgtgccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAAc                                                                                       <  2:415053/140‑1 (MQ=255)

                                                 gccatcgtgccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAAc                                                                                       <  2:336427/140‑1 (MQ=255)

                                                   catcgtgccatcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGt                                                                                     >  1:1063003/1‑140 (MQ=255)

                                                           catcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGa                                                                             <  1:1411422/140‑1 (MQ=255)

                                                           catcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGa                                                                             <  1:438249/140‑1 (MQ=255)

                                                           catcgtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGa                                                                             <  2:391763/140‑1 (MQ=255)

                                                               gtCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGAt                                                                         >  1:1329547/1‑140 (MQ=255)

                                                                         aGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGa                                                               <  1:577311/140‑1 (MQ=255)

                                                                           cTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACg                                                             >  1:585911/1‑140 (MQ=255)

                                                                           cTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACg                                                             >  1:1121272/1‑140 (MQ=255)

                                                                           cTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACg                                                             >  2:1375141/1‑140 (MQ=255)

                                                                              acacaGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGtt                                                          <  1:636585/140‑1 (MQ=255)

                                                                                    tACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTgcgc                                                    >  1:553887/1‑140 (MQ=255)

                                                                                     acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGcc                                                   >  1:847208/1‑140 (MQ=255)

                                                                                     acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGcc                                                   >  1:700383/1‑140 (MQ=255)

                                                                                     acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGcc                                                   >  1:64046/1‑140 (MQ=255)

                                                                                     acacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGcc                                                   >  2:1097612/1‑140 (MQ=255)

                                                                                      cacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCa                                                  >  1:1313864/1‑140 (MQ=255)

                                                                                      cacCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCa                                                  <  1:41815/140‑1 (MQ=255)

                                                                                            ccGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCa                                            >  2:150514/1‑140 (MQ=255)

                                                                                            ccGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCa                                            >  1:1016991/1‑140 (MQ=255)

                                                                                            ccGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCa                                            >  1:1249803/1‑140 (MQ=255)

                                                                                            ccGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCa                                            >  1:1460981/1‑140 (MQ=255)

                                                                                            ccGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCa                                            >  1:133561/1‑140 (MQ=255)

                                                                                            ccGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGC‑TCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCaa                                           >  2:1219165/1‑140 (MQ=255)

                                                                                              ggTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAAt                                          >  2:536503/1‑140 (MQ=255)

                                                                                                tttAAAGCCTGCCAGCGTCACCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCgg                                              <  2:520052/134‑1 (MQ=255)

                                                                                                tttAAAGCCTGCCAGCGTCACCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCgg                                              >  1:520052/1‑134 (MQ=255)

                                                                                                        cTGCCAGCGTCATCAGATCAATAGTTGC‑TCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGaa                               <  1:1219165/140‑1 (MQ=255)

                                                                                                         tGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGaa                               <  2:1063003/140‑1 (MQ=255)

                                                                                                         tGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGaa                               <  1:313561/140‑1 (MQ=255)

                                                                                                         tGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGaa                               <  1:868819/140‑1 (MQ=255)

                                                                                                         tGCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGaa                               >  2:868819/1‑140 (MQ=255)

                                                                                                          gCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAACCGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAAc                              >  1:110383/1‑140 (MQ=255)

                                                                                                          gCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAAc                              >  2:870032/1‑140 (MQ=255)

                                                                                                          gCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAAc                              <  2:375855/140‑1 (MQ=255)

                                                                                                          gCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAAc                              >  2:795769/1‑140 (MQ=255)

                                                                                                          gCCAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAAc                              <  2:1121272/140‑1 (MQ=255)

                                                                                                            cAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACgg                            <  1:795769/140‑1 (MQ=255)

                                                                                                            cAGCGTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACgg                            <  2:1313864/140‑1 (MQ=255)

                                                                                                                gTCATCAGATCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGt                        >  2:547840/1‑140 (MQ=255)

                                                                                                                        aTCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGt                >  2:970904/1‑140 (MQ=255)

                                                                                                                        aTCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGt                <  1:517104/140‑1 (MQ=255)

                                                                                                                        aTCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGt                <  2:133561/140‑1 (MQ=255)

                                                                                                                        aTCAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGt                <  2:1249803/140‑1 (MQ=255)

                                                                                                                          cAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGaa                               <  2:358653/123‑1 (MQ=255)

                                                                                                                          cAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCa              >  2:700183/1‑140 (MQ=255)

                                                                                                                          cAATAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCa              >  1:919944/1‑140 (MQ=255)

                                                                                                                             tAGTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCg           >  1:1091557/1‑140 (MQ=255)

                                                                                                                               gTTGCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGc         <  2:1329547/140‑1 (MQ=255)

                                                                                                                                  gCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAg             <  1:1107142/133‑1 (MQ=255)

                                                                                                                                  gCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAg             >  2:1107142/1‑133 (MQ=255)

                                                                                                                                  gCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGa      >  2:974303/1‑140 (MQ=255)

                                                                                                                                  gCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGa      >  1:1186792/1‑140 (MQ=255)

                                                                                                                                  gCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGa      >  1:1233905/1‑140 (MQ=255)

                                                                                                                                  gCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGa      >  1:314469/1‑140 (MQ=255)

                                                                                                                                  gCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGa      >  1:530865/1‑140 (MQ=255)

                                                                                                                                  gCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGa      >  1:560390/1‑140 (MQ=255)

                                                                                                                                  gCTTCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGa      >  1:591183/1‑140 (MQ=255)

                                                                                                                                    ttCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGaaa    <  1:974303/140‑1 (MQ=255)

                                                                                                                                     tCAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAac   >  2:545174/1‑140 (MQ=255)

                                                                                                                                      cAGCATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAaca  <  2:1460981/140‑1 (MQ=255)

GAGCGCCATATTGTGTTTATTGGCAAACTCAATACACTCTGGTGCACGCGCCATCGTGCCATCGTCATTAGTCAGCTCACACAGTACACCAGCCGGTTTAAAGCCTGCCAGCGTCATCAGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACA  >  NC_000913/3183870‑3184143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`