Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,189,688	A→G	pseudogene (2257/2434 nt)	yqiG →	putative outer membrane usher protein YqiG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,189,688	0	A	G	100.0%	303.7 / NA	91	pseudogene (2257/2434 nt)	yqiG	putative outer membrane usher protein YqiG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/48);  total (43/48)

TCGGTTATCACCACCACCTTAACCGAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAAACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAACCACCCAATAGCAGCAACTT  >  NC_000913/3189551‑3189822                                                                                                                                          |                                                                                                                                       tCGGTTATCACCACCACCTTAACCGAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGAc                                                                                                                                      <  2:1347417/140‑1 (MQ=255) tCGGTTATCACCACCACCTTAACCGAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGAc                                                                                                                                      <  2:215237/140‑1 (MQ=255)         caccaccacCTTAACCGAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCcaca                                                                                                                              <  2:518981/140‑1 (MQ=255)          accaccaccTTAACCGAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACaa                                                                                                                             <  1:928054/140‑1 (MQ=255)          accaccaccTTAACCGAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACaa                                                                                                                             <  1:673605/140‑1 (MQ=255)             accaccTTAACCGAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACaa                                                                                                                             >  2:1333524/1‑137 (MQ=255)             accaccTTAACCGAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACaa                                                                                                                             <  1:1333524/137‑1 (MQ=255)             accaccTTAACCGAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGa                                                                                                                          <  1:622593/140‑1 (MQ=255)               caccTTAACCGAGGGTGCTATTGGCTACGCCAAACTTCATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACaa                                                                                                                             <  1:1184318/135‑1 (MQ=255)               caccTTAACCGAGGGTGCTATTGGCTACGCCAAACTTCATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACaa                                                                                                                             >  2:1184318/1‑135 (MQ=255)                accTTAACCGAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAAtt                                                                                                                       <  1:295266/140‑1 (MQ=255)                 ccTTAACCGAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTa                                                                                                                      >  2:526073/1‑140 (MQ=255)                   ttAACCGAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAgg                                                                                                                    <  2:845489/140‑1 (MQ=255)                    tAACCGAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGa                                                                                                                   >  2:78436/1‑140 (MQ=255)                         gAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACTAAGAATTAGGACTGGt                                                                                                              >  2:1062656/1‑140 (MQ=255)                         gAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGt                                                                                                              >  2:1457717/1‑140 (MQ=255)                          aGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGtt                                                                                                             >  1:348992/1‑140 (MQ=255)                            ggTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGc                                                                                                           >  2:1126746/1‑140 (MQ=255)                            ggTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGc                                                                                                           >  1:58569/1‑140 (MQ=255)                             gTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCt                                                                                                          <  2:58569/140‑1 (MQ=255)                              tGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTg                                                                                                         >  2:916908/1‑140 (MQ=255)                                cTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGAt                                                                                                       >  2:1036428/1‑140 (MQ=255)                                cTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGAt                                                                                                       >  2:181915/1‑140 (MQ=255)                                 tATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATg                                                                                                      >  2:1241830/1‑140 (MQ=255)                                  aTTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGTTTATTCGCCTGACAGATGGTAGTCATCCACCACGGGGGATTAGCGTAAAAGATGAGTCCAGCCACAAAGAATTAGGACTGGTTGCTGATgg                                                                                                     <  2:1364236/140‑1 (MQ=255)                                  aTTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATgg                                                                                                     <  1:483224/140‑1 (MQ=255)                                  aTTGCCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATgg                                                                                                     <  2:1353697/140‑1 (MQ=255)                                    tGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATggcg                                                                                                   <  1:1218293/140‑1 (MQ=255)                                     ggCTACGCCAAACTTGATCCCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATggcgg                                                                                                  <  1:1433455/140‑1 (MQ=255)                                     ggCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATggcgg                                                                                                  >  2:1088620/1‑140 (MQ=255)                                     ggCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATggcgg                                                                                                  <  1:916908/140‑1 (MQ=255)                                      gCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATggcggc                                                                                                 >  1:636337/1‑140 (MQ=255)                                        tACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCtt                                                                                               >  1:389719/1‑140 (MQ=255)                                        tACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATAAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATgg                                                                                                     <  2:538827/134‑1 (MQ=255)                                        tACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATAAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATgg                                                                                                     >  1:538827/1‑134 (MQ=255)                                          cGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGt                                                                                                              >  2:1294599/1‑123 (MQ=255)                                          cGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGt                                                                                                              <  1:1294599/123‑1 (MQ=255)                                          cGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATgg                                                                                                     >  2:125791/1‑132 (MQ=255)                                          cGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATgg                                                                                                     <  1:125791/132‑1 (MQ=255)                                          cGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATgg                                                                                                     >  2:1090325/1‑132 (MQ=255)                                          cGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATgg                                                                                                     <  1:1090325/132‑1 (MQ=255)                                          cGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTg                                                                                             <  1:78436/140‑1 (MQ=255)                                           gCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGt                                                                                            >  1:632681/1‑140 (MQ=255)                                               aaCTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATAc                                                                                        >  1:170195/1‑140 (MQ=255)                                                      tACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACg                                                                                 <  1:1243116/140‑1 (MQ=255)                                                      tACCCGCAAGGGCCACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACg                                                                                 <  2:482789/140‑1 (MQ=255)                                                         ccGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCa                                                                              <  1:484019/140‑1 (MQ=255)                                                              aGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACTAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAg                                                                         <  1:1062656/140‑1 (MQ=255)                                                               gggCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAgg                                                                        <  2:636337/140‑1 (MQ=255)                                                                ggCTCCCAAATCATAGGGGTTTTTCGCCTGGCAGATGGTAGTGATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGga                                                                       <  2:1370169/140‑1 (MQ=255)                                                                    aCCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGgatgat                                                                   <  2:348992/140‑1 (MQ=255)                                                                           cATAGGGGTTATTCGCCTGGCAGATGGAAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAAc                                                            >  2:1287874/1‑140 (MQ=255)                                                                                gggTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCt                                                       <  2:170195/140‑1 (MQ=255)                                                                                   ttATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCt                                                       >  1:1129982/1‑137 (MQ=255)                                                                                   ttATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCt                                                       <  2:1129982/137‑1 (MQ=255)                                                                                   ttATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTa                                                    >  1:120879/1‑140 (MQ=255)                                                                                   ttATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTa                                                    >  1:276643/1‑140 (MQ=255)                                                                                          ccTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAAc                                                            >  1:1419027/1‑125 (MQ=255)                                                                                          ccTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAAc                                                            <  2:1419027/125‑1 (MQ=255)                                                                                          ccTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTggg                                             >  1:616644/1‑140 (MQ=255)                                                                                           cTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTgggg                                            >  2:8614/1‑140 (MQ=255)                                                                                                  aTGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCt                                                >  1:65364/1‑129 (MQ=255)                                                                                                  aTGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCt                                                <  2:65364/129‑1 (MQ=255)                                                                                                  aTGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACaaa                                     >  1:515287/1‑140 (MQ=255)                                                                                                    ggTAGTCATCCACCACTGGGGATTAGCGTAAAAGGTGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATc                                   <  1:1164582/140‑1 (MQ=255)                                                                                                    ggTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATc                                   <  2:276643/140‑1 (MQ=255)                                                                                                       aGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTg                                >  1:531002/1‑140 (MQ=255)                                                                                                       aGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTg                                <  2:515287/140‑1 (MQ=255)                                                                                                       aGTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTg                                <  2:389719/140‑1 (MQ=255)                                                                                                        gTCATCCACCACTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGt                               >  2:905994/1‑140 (MQ=255)                                                                                                                 cacTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCaa                      >  1:660076/1‑140 (MQ=255)                                                                                                                  acTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAac                     <  1:1241830/140‑1 (MQ=255)                                                                                                                  acTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAac                     <  2:120879/140‑1 (MQ=255)                                                                                                                  acTGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAac                     <  1:997936/140‑1 (MQ=255)                                                                                                                    tGGGGATTAGCGTAAAAGATGAGCCCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAac                     <  2:641787/138‑1 (MQ=255)                                                                                                                    tGGGGATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAac                     >  1:641787/1‑138 (MQ=255)                                                                                                                     ggggATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAaccac                  <  1:181915/140‑1 (MQ=255)                                                                                                                     ggggATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAaccac                  >  1:1015274/1‑140 (MQ=255)                                                                                                                      gggATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAacca                   >  2:66932/1‑138 (MQ=255)                                                                                                                      gggATTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAacca                   <  1:66932/138‑1 (MQ=255)                                                                                                                         aTTAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAACCACCCaa              <  2:1015274/140‑1 (MQ=255)                                                                                                                           tAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAACCACCCAATa            >  1:1136568/1‑140 (MQ=255)                                                                                                                           tAGCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAACCACCCAATa            >  1:33328/1‑140 (MQ=255)                                                                                                                             gCGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAACCACCCAATagc          >  2:1120981/1‑140 (MQ=255)                                                                                                                              cGTAAAAGGTGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAACCACCCAATagca         >  2:1001388/1‑140 (MQ=255)                                                                                                                              cGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAACCACCCAATagca         <  2:632681/140‑1 (MQ=255)                                                                                                                              cGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAACCACCCAATagca         >  1:1436869/1‑140 (MQ=255)                                                                                                                              cGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAACCACCCAATagca         <  2:477699/140‑1 (MQ=255)                                                                                                                              cGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAACCACCCAATagca         <  1:1457717/140‑1 (MQ=255)                                                                                                                              cGTAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAACCACCCAATagca         <  1:905994/140‑1 (MQ=255)                                                                                                                                tAAAAGATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAACCACCCAATagcagc       >  1:764557/1‑140 (MQ=255)                                                                                                                                     gATGAGACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAACCACCCAATAGCAGCAACtt  <  2:531002/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TCGGTTATCACCACCACCTTAACCGAGGGTGCTATTGGCTACGCCAAACTTGATACCCGCAAGGGCTACCAAATCATAGGGGTTATTCGCCTGGCAGATGGTAGTCATCCACCACTGGGGATTAGCGTAAAAGATGAAACCAGCCACAAAGAATTAGGACTGGTTGCTGATGGCGGCTTTGTATACCTCAACGGCATTCAGGATGATAACAAACTTGCTTTACGCTGGGGTGACAAATCTTGTTTTATTCAACCACCCAATAGCAGCAACTT  >  NC_000913/3189551‑3189822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑