Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,229,143	T→C	Y86H (TAC→CAC)	ygjK →	glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,229,143	0	T	C	100.0%	245.2 / NA	71	Y86H (TAC→CAC)	ygjK	glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/35);  total (36/35)

ATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTGACAGCAAAAGATGTGCAAGT  >  NC_000913/3229008‑3229276                                                                                                                                        |                                                                                                                                      aTGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACAt                                                                                                                                   <  2:887731/140‑1 (MQ=255)   gAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCa                                                                                                                                 >  1:130769/1‑140 (MQ=255)   gAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCa                                                                                                                                 >  1:79457/1‑140 (MQ=255)   gAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCa                                                                                                                                 <  1:442906/140‑1 (MQ=255)   gAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCa                                                                                                                                 <  2:2444/140‑1 (MQ=255)        attacgattacgACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACttt                                                                                                                            <  2:985582/140‑1 (MQ=255)        attacgattacgACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACttt                                                                                                                            >  2:944546/1‑140 (MQ=255)          tacgattacgaCGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTAt                                                                                                                          >  1:1383564/1‑140 (MQ=255)          tacgattacgaCGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTAt                                                                                                                          >  2:263766/1‑140 (MQ=255)                 acgacgATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAg                                                                                                                    >  2:219253/1‑139 (MQ=255)                 acgacgATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAg                                                                                                                    <  1:219253/139‑1 (MQ=255)                       aTCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTc                                                                                                             >  2:768989/1‑140 (MQ=255)                       aTCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTc                                                                                                             >  2:10982/1‑140 (MQ=255)                       aTCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTc                                                                                                             >  2:783721/1‑140 (MQ=255)                        tCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCg                                                                                                            >  1:813836/1‑140 (MQ=255)                               cGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCt                                                                                                     <  1:768989/140‑1 (MQ=255)                                gTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTg                                                                                                    >  1:186365/1‑140 (MQ=255)                                  tttAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGAc                                                                                                  <  1:944546/140‑1 (MQ=255)                                    tAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCg                                                                                                <  2:1383564/140‑1 (MQ=255)                                      aTCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGTGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTc                                                                                              >  2:502374/1‑140 (MQ=255)                                      aTCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTc                                                                                              >  1:935232/1‑140 (MQ=255)                                         cGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTgg                                                                                           >  2:18811/1‑140 (MQ=255)                                          gTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGc                                                                                          <  2:117859/140‑1 (MQ=255)                                                     tcGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAg                                                                               <  2:130769/140‑1 (MQ=255)                                                          gCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGt                                                                          <  1:10982/140‑1 (MQ=255)                                                          gCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGt                                                                          <  2:186365/140‑1 (MQ=255)                                                               gCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAg                                                                               >  2:983118/1‑130 (MQ=255)                                                               gCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAg                                                                               <  1:983118/130‑1 (MQ=255)                                                                     tCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGc                                                               <  1:18811/140‑1 (MQ=255)                                                                     tCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGc                                                               <  2:310193/140‑1 (MQ=255)                                                                     tCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGc                                                               <  2:1282792/140‑1 (MQ=255)                                                                       aTCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTg                                                             <  2:480842/140‑1 (MQ=255)                                                                         cTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGa                                                           >  2:486280/1‑140 (MQ=255)                                                                          tGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAg                                                          <  2:1187437/140‑1 (MQ=255)                                                                          tGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAg                                                          <  1:1389064/140‑1 (MQ=255)                                                                          tGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACCCTGGAg                                                          <  1:1052818/140‑1 (MQ=255)                                                                          tGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACAGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAg                                                          <  2:1222098/140‑1 (MQ=255)                                                                             tGCCAGACGGCCCTAACACCATGGGTGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCa                                                       <  1:502374/140‑1 (MQ=255)                                                                              gCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCAt                                                      >  1:1139356/1‑140 (MQ=255)                                                                               ccAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATa                                                     >  1:163692/1‑140 (MQ=255)                                                                                cAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATAc                                                    <  2:163692/140‑1 (MQ=255)                                                                                 aGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACa                                                   >  2:951783/1‑140 (MQ=255)                                                                                   aCGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGa                                                           <  1:233710/130‑1 (MQ=255)                                                                                   aCGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGa                                                           >  2:233710/1‑130 (MQ=255)                                                                                   aCGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGt                                                 >  2:849403/1‑140 (MQ=255)                                                                                    cGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTa                                                >  1:534434/1‑140 (MQ=255)                                                                                        ccTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTcc                                            <  2:935232/140‑1 (MQ=255)                                                                                            acacCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGt                                        >  1:165615/1‑140 (MQ=255)                                                                                               ccATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTgcg                                     <  1:783721/140‑1 (MQ=255)                                                                                                 aTGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTTGAGGCATACAGTATTCCCGGTGCGCt                                   >  2:168449/1‑140 (MQ=255)                                                                                                 aTGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTAt                                               >  2:1321905/1‑128 (MQ=255)                                                                                                 aTGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTAt                                               <  1:1321905/128‑1 (MQ=255)                                                                                                 aTGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCt                                   <  1:916245/140‑1 (MQ=255)                                                                                                 aTGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCt                                   >  1:600025/1‑140 (MQ=255)                                                                                                 aTGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCt                                   >  2:1408609/1‑140 (MQ=255)                                                                                                 aTGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCt                                   >  2:916245/1‑140 (MQ=255)                                                                                                  tGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTg                                  >  2:958575/1‑140 (MQ=255)                                                                                                  tGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTg                                  >  2:1357257/1‑140 (MQ=255)                                                                                                  tGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTg                                  >  2:1380924/1‑140 (MQ=255)                                                                                                    ggcggcTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGGCGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGt                                >  2:166570/1‑140 (MQ=255)                                                                                                     gcggcTTTCCGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTg                               >  2:552490/1‑140 (MQ=255)                                                                                                             ccGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACt                       <  1:951783/140‑1 (MQ=255)                                                                                                              cGGGCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTg                      <  1:1357257/140‑1 (MQ=255)                                                                                                               gggCGTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGGCGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTGa                     <  1:166570/140‑1 (MQ=255)                                                                                                                   gTTGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTGACAGc                 <  1:958575/140‑1 (MQ=255)                                                                                                                     tGCGCTGCTGACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTGACAGCaa               >  1:828598/1‑140 (MQ=255)                                                                                                                           gctgACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTGACAGCAAAAGAtg         >  1:771384/1‑140 (MQ=255)                                                                                                                            ctgACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTGACAGCAAAAGAtgt        >  2:1382662/1‑140 (MQ=255)                                                                                                                             tgACGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTGACAGCAAAAGAtgtg       <  2:600025/140‑1 (MQ=255)                                                                                                                               aCGGAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTGACAGCAAAAGATGTGCa     >  2:493021/1‑140 (MQ=255)                                                                                                                                  gAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTGACAGCAAAAGATGTGCAAGt  <  2:828598/140‑1 (MQ=255)                                                                                                                                  gAAGAGCACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTGACAGCAAAAGATGTGCAAGt  <  2:534434/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      ATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTGACAGCAAAAGATGTGCAAGT  >  NC_000913/3229008‑3229276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑