Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 157,460 T→C G91G (GGA→GGG)  folK ← 2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine diphosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913157,4600TC100.0% 349.6 / NA 101G91G (GGA→GGG) folK2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine diphosphokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/50);  total (51/50)

Only 100 of 104 total aligned reads displayed.

ACCAACTCCGGCGCGATTTCAAACAGCGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGTCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTGATCTTGCGGCCC  >  NC_000913/157323‑157594
                                                                                                                                         |                                                                                                                                      
acCAACTCCGGCGCGATTTCAAACAGCGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGccc                                                                                                                                      >  2:900362/1‑140 (MQ=255)
acCAACTCCGGCGCGATTTCAAACAGCGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGccc                                                                                                                                      >  2:427373/1‑140 (MQ=255)
     cTCCGGCGCGATTTCAAACAGCGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGc                                                                                                                                 >  1:205687/1‑140 (MQ=255)
        cGGCGCGATTTCAAACAGCGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGtt                                                                                                                              <  1:869417/140‑1 (MQ=255)
        cGGCGCGATTTCAAACAGCGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGtt                                                                                                                              >  1:528333/1‑140 (MQ=255)
            gcgATTTCAAACAGCGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGc                                                                                                                          >  1:436400/1‑140 (MQ=255)
                tttCAAACAGCGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTg                                                                                                                      >  2:65678/1‑140 (MQ=255)
                    aaaCAGCGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGa                                                                                                                  <  2:528333/140‑1 (MQ=255)
                      aCAGCGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGAcg                                                                                                                <  1:91385/140‑1 (MQ=255)
                      aCAGCGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGAcg                                                                                                                <  1:65678/140‑1 (MQ=255)
                       cAGCGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGAcgc                                                                                                               <  1:681269/140‑1 (MQ=255)
                         gCGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGa                                                                                                             <  2:359141/140‑1 (MQ=255)
                          cGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGAc                                                                                                            >  2:1412693/1‑140 (MQ=255)
                          cGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCCAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGAc                                                                                                            >  2:594328/1‑140 (MQ=255)
                          cGGCCACAGCATAAATCCACGATCCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACT‑‑‑‑‑‑‑‑‑‑ACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCa                                                                                                   >  1:1126402/1‑139 (MQ=255)
                            gCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGa                                                                                                                  <  1:416502/132‑1 (MQ=255)
                            gCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCt                                                                                                          >  2:1012946/1‑140 (MQ=255)
                                 aGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTtgctg                                                                                                     <  1:78799/140‑1 (MQ=255)
                                 aGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTtgctg                                                                                                     <  2:549047/140‑1 (MQ=255)
                                  gCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTtgctgc                                                                                                    >  1:37788/1‑140 (MQ=255)
                                   cATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCa                                                                                                   <  2:1278241/140‑1 (MQ=255)
                                     tAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAAt                                                                                                 <  1:602913/140‑1 (MQ=255)
                                         tCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCaa                                                                                             <  2:40793/140‑1 (MQ=255)
                                             cGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGTGACCTTGCTGCAATTCAATACg                                                                                         <  2:1303682/140‑1 (MQ=255)
                                             cGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACg                                                                                         <  1:202564/140‑1 (MQ=255)
                                             cGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACg                                                                                         >  2:469374/1‑140 (MQ=255)
                                             cGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACg                                                                                         <  2:205687/140‑1 (MQ=255)
                                             cGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACg                                                                                         <  2:427944/140‑1 (MQ=255)
                                             cGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACg                                                                                         <  2:37788/140‑1 (MQ=255)
                                               aTTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGGGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCt                                                                                       <  1:1103928/140‑1 (MQ=255)
                                                  cttcATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCtgtg                                                                                    >  1:1062552/1‑140 (MQ=255)
                                                  cttcATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCCAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCtgtg                                                                                    <  1:594328/140‑1 (MQ=255)
                                                   ttcATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCtgtgt                                                                                   >  1:94907/1‑140 (MQ=255)
                                                       tatCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGa                                                                               <  1:427373/140‑1 (MQ=255)
                                                       tatCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGa                                                                               <  2:34163/140‑1 (MQ=255)
                                                             aGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGTGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGt                                                                         <  2:485888/140‑1 (MQ=255)
                                                             aGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACg                                                                                         <  2:385743/124‑1 (MQ=255)
                                                             aGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACg                                                                                         >  1:385743/1‑124 (MQ=255)
                                                             aGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGt                                                                         <  2:436400/140‑1 (MQ=255)
                                                              gTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTa                                                                        >  2:759078/1‑140 (MQ=255)
                                                                gCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGGAATTCAATACGCTGTGTGTGATTGAGTAGc                                                                      >  2:901760/1‑140 (MQ=255)
                                                                 cGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGa                                                                               <  2:436433/130‑1 (MQ=255)
                                                                 cGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGa                                                                               >  1:436433/1‑130 (MQ=255)
                                                                 cGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGct                                                                     >  1:180624/1‑140 (MQ=255)
                                                                 cGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGct                                                                     >  2:341596/1‑140 (MQ=255)
                                                                 cGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGct                                                                     >  2:204016/1‑140 (MQ=255)
                                                                    aaCGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAg                                                                       >  1:674753/1‑135 (MQ=255)
                                                                    aaCGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAg                                                                       <  2:674753/135‑1 (MQ=255)
                                                                    aaCGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCtt                                                                  >  2:848288/1‑140 (MQ=255)
                                                                     aCGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTc                                                                 <  1:848288/140‑1 (MQ=255)
                                                                        gTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGt                                                                         <  2:249506/129‑1 (MQ=255)
                                                                        gTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGt                                                                         >  1:249506/1‑129 (MQ=255)
                                                                         tCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGt                                                             >  1:54760/1‑140 (MQ=255)
                                                                         tCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGt                                                             >  2:183759/1‑140 (MQ=255)
                                                                         tCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGt                                                             >  1:861122/1‑140 (MQ=255)
                                                                             gCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCaa                                                         <  2:861122/140‑1 (MQ=255)
                                                                             gCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCaa                                                         <  2:430156/140‑1 (MQ=255)
                                                                             gCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCaa                                                         <  1:759078/140‑1 (MQ=255)
                                                                              cGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTGGCTCTTCAGGTGCAag                                                        >  2:387326/1‑140 (MQ=255)
                                                                              cGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAag                                                        >  1:199912/1‑140 (MQ=255)
                                                                                   aGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAgg                                                   >  2:1289466/1‑140 (MQ=255)
                                                                                   aGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAgg                                                   >  1:538483/1‑140 (MQ=255)
                                                                                     tATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAgg                                                              <  2:80757/127‑1 (MQ=255)
                                                                                     tATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAgg                                                              >  1:80757/1‑127 (MQ=255)
                                                                                     tATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGtt                                                 >  2:223500/1‑140 (MQ=255)
                                                                                       tttATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTc                                               >  1:796106/1‑140 (MQ=255)
                                                                                          aTCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAg                                            <  1:223500/140‑1 (MQ=255)
                                                                                          aTCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAg                                            >  1:1318534/1‑140 (MQ=255)
                                                                                               ttCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGTGCCa                                       >  1:329133/1‑140 (MQ=255)
                                                                                               ttCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCa                                       >  2:617525/1‑140 (MQ=255)
                                                                                               ttCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCa                                       >  2:843284/1‑140 (MQ=255)
                                                                                                  aTTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACgg                                    <  1:1412693/140‑1 (MQ=255)
                                                                                                  aTTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACgg                                    <  1:183759/140‑1 (MQ=255)
                                                                                                   ttACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGc                                   <  2:373263/140‑1 (MQ=255)
                                                                                                    tACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCt                                  >  2:187859/1‑140 (MQ=255)
                                                                                                         aaCAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGtt                             >  2:647982/1‑140 (MQ=255)
                                                                                                           cAGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTa                           >  2:499828/1‑140 (MQ=255)
                                                                                                            aGCATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTaa                          >  2:430799/1‑140 (MQ=255)
                                                                                                              cATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGt                        >  1:1344581/1‑140 (MQ=255)
                                                                                                              cATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGt                        >  2:612394/1‑140 (MQ=255)
                                                                                                              cATGATGTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGt                        >  1:988310/1‑140 (MQ=255)
                                                                                                                 gatgTCGAGATCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAAt                     <  2:1062552/140‑1 (MQ=255)
                                                                                                                       gagaTCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGtt               >  2:402172/1‑140 (MQ=255)
                                                                                                                        agaTCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTg              >  2:122185/1‑140 (MQ=255)
                                                                                                                        agaTCCAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTg              >  1:632345/1‑140 (MQ=255)
                                                                                                                            ccAGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTGATCt          <  2:1318534/140‑1 (MQ=255)
                                                                                                                              aGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTGATCTTg        <  1:843284/140‑1 (MQ=255)
                                                                                                                              aGCGTGCGTGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTGATCTTg        <  1:901760/140‑1 (MQ=255)
                                                                                                                               gcgtgcgtGGCCTCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAAt                     >  2:1137833/1‑126 (MQ=255)
                                                                                                                               gcgtgcgtGGCCCCCAGCGTTCAGCTTTGCGGACGCGACTTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTGATCTTGc       >  2:123070/1‑140 (MQ=255)
                                                                                                                               gcgtgcgtGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAAt                     <  1:1137833/126‑1 (MQ=255)
                                                                                                                               gcgtgcgtGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTGATCTTGc       <  2:180624/140‑1 (MQ=255)
                                                                                                                               gcgtgcgtGGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTGATCTTGc       >  2:1082674/1‑140 (MQ=255)
                                                                                                                                cgtgcgtgGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTg              <  2:1444496/132‑1 (MQ=255)
                                                                                                                                cgtgcgtgGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTg              >  1:1444496/1‑132 (MQ=255)
                                                                                                                                    ggtgGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTGATCTTGCGGccc  <  1:1426887/139‑1 (MQ=255)
                                                                                                                                    cgtgGCCCCCAGCGTTCAGCTTTGCGGACGCGACTTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTGATCTTGCGGccc  <  1:123070/140‑1 (MQ=255)
                                                                                                                                    cgtgGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTGATCTTGCgg     >  2:686726/1‑137 (MQ=255)
                                                                                                                                    cgtgGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTGATCTTGCgg     <  1:686726/137‑1 (MQ=255)
                                                                                                                                    cgtgGCCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTGATCTTGCGGccc  <  1:1082674/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
ACCAACTCCGGCGCGATTTCAAACAGCGGCCACAGCATAAATCCACGATTCTTCATATCGTAGTGCGGAACGGTCAGGCGTTCAGTATTTATCACTTCATTACCAAACAGCATGATGTCGAGATCCAGCGTGCGTGGTCCCCAGCGTTCAGCTTTGCGGACGCGACCTTGCTGCAATTCAATACGCTGTGTGTGATTGAGTAGCTCTTCAGGTGCAAGAGAGGTTTCCAGCGCCACGGCTGCGTTTAAGTAATCGGGTTGATCTTGCGGCCC  >  NC_000913/157323‑157594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑