Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,279,346	G→A	A145T (GCT→ACT)	kbaZ →	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,279,346	0	G	A	100.0%	200.2 / NA	58	A145T (GCT→ACT)	kbaZ	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/30);  total (28/30)

CCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGG  >  NC_000913/3279214‑3279481                                                                                                                                     |                                                                                                                                        ccggccgCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAAcg                                                                                                                                  <  2:473482/140‑1 (MQ=255)      cgCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGcgccg                                                                                                                             <  2:228237/140‑1 (MQ=255)                  ggCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGa                                                                                                                 >  2:50029/1‑140 (MQ=255)                  ggCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGa                                                                                                                 <  1:442310/140‑1 (MQ=255)                             atgatTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGgaa                                                                                                      >  1:889838/1‑140 (MQ=255)                             atgatTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGgaa                                                                                                      <  1:715092/140‑1 (MQ=255)                             atgatTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGgaa                                                                                                      >  2:532311/1‑140 (MQ=255)                             atgatTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGgaa                                                                                                      >  1:1133359/1‑140 (MQ=255)                             atgatTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGgaa                                                                                                      >  2:1203375/1‑140 (MQ=255)                                   tgattAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAAcc                                                                                                >  1:976404/1‑140 (MQ=255)                                    gattAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCt                                                                                               <  1:296585/140‑1 (MQ=255)                                    gattAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCt                                                                                               <  1:50029/140‑1 (MQ=255)                                    gattAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCt                                                                                               >  2:946455/1‑140 (MQ=255)                                       tAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTc                                                                                            <  2:889838/140‑1 (MQ=255)                                          aaGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGGGGCGGAAGAAACCTTTCttt                                                                                         >  2:305229/1‑139 (MQ=255)                                           aGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGa                                                                                        <  2:1393340/140‑1 (MQ=255)                                            gCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGaa                                                                                       <  2:577010/140‑1 (MQ=255)                                               aCGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAacac                                                                                    >  2:761822/1‑140 (MQ=255)                                               aCGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAacac                                                                                    >  2:1183069/1‑140 (MQ=255)                                               aCGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAacac                                                                                    <  1:98851/140‑1 (MQ=255)                                                     cGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGc                                                                              >  1:839166/1‑140 (MQ=255)                                                     cGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGc                                                                              <  1:946455/140‑1 (MQ=255)                                                        cAGGATTC‑AAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAg                                                                          <  1:1461523/140‑1 (MQ=255)                                                         aGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAg                                                                          <  1:1203375/140‑1 (MQ=255)                                                          ggATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGc                                                                         >  2:518353/1‑140 (MQ=255)                                                          ggATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGc                                                                         <  1:173818/140‑1 (MQ=255)                                                           gATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGcc                                                                        >  1:147938/1‑140 (MQ=255)                                                                 aaaaaaTCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTg                                                                  <  2:839166/140‑1 (MQ=255)                                                                 aaaaaaTCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTg                                                                  <  2:1133359/140‑1 (MQ=255)                                                                   aaaaTCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGa                                                                <  2:568679/140‑1 (MQ=255)                                                                   aaaaTCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGa                                                                <  1:518353/140‑1 (MQ=255)                                                                    aaaTCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAg                                                               <  2:976404/140‑1 (MQ=255)                                                                     aaTCCACCTTGATTGCAGCATCTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGt                                                              <  2:1340354/140‑1 (MQ=255)                                                                      aTCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTa                                                             >  1:1340086/1‑140 (MQ=255)                                                                            cTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCAt                                                       <  2:975423/140‑1 (MQ=255)                                                                               gATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTgg                                                    >  2:136109/1‑140 (MQ=255)                                                                                     agcaTGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTAt                                                            <  1:487157/126‑1 (MQ=255)                                                                                     agcaTGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTAt                                                            >  2:487157/1‑126 (MQ=255)                                                                                       caTGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAg                                            <  1:532311/140‑1 (MQ=255)                                                                                          gTCCTGTCAGGACGATCCGATCCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGc                                         <  2:1340086/140‑1 (MQ=255)                                                                                           tCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGa                                              <  2:1307783/134‑1 (MQ=255)                                                                                           tCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGa                                              >  1:1307783/1‑134 (MQ=255)                                                                                           tCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGcc                                        >  2:205032/1‑140 (MQ=255)                                                                                            ccTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCg                                       <  2:147938/140‑1 (MQ=255)                                                                                                     aCGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTggc                              >  1:308957/1‑140 (MQ=255)                                                                                                     aCGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTggc                              >  1:289281/1‑140 (MQ=255)                                                                                                     aCGATCCGATTCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGGATTGCATTGGTACCGAAGTGCCGGTACCCggc                              >  2:211046/1‑140 (MQ=255)                                                                                                               tCCCTTAACTGATGACATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATg                    <  1:205032/140‑1 (MQ=255)                                                                                                                         gatgaCATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAg          <  1:136109/140‑1 (MQ=255)                                                                                                                          atgaCATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGc         >  2:965325/1‑140 (MQ=255)                                                                                                                           tgaCATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCg        >  2:1352402/1‑140 (MQ=255)                                                                                                                           tgaCATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCg        >  2:449376/1‑140 (MQ=255)                                                                                                                           tgaCATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCg        >  2:912763/1‑140 (MQ=255)                                                                                                                             aCATCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAg      <  1:211046/140‑1 (MQ=255)                                                                                                                               aTCGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCt    >  2:234430/1‑140 (MQ=255)                                                                                                                                 cGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAg      >  2:78588/1‑136 (MQ=255)                                                                                                                                 cGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAg      <  1:78588/136‑1 (MQ=255)                                                                                                                                 cGTGACTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTgg  >  2:988979/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        CCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGG  >  NC_000913/3279214‑3279481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑