BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,345,712	T→C	S332S (AGT→AGC)	rpoN →	RNA polymerase, sigma 54 (sigma N) factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,345,712	0	T	C	100.0%	366.8 / NA	105	S332S (AGT→AGC)	rpoN	RNA polymerase, sigma 54 (sigma N) factor
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (51/54); total (51/54)

Only 100 of 106 total aligned reads displayed.

GAACTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTGTCGAAATGCATGAAT  >  NC_000913/3345578‑3345851

gAACTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGa                                                                                                                                        <  2:348647/140‑1 (MQ=255)

 aaCTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGaa                                                                                                                                       >  2:1392900/1‑140 (MQ=255)

   cTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAg                                                                                                                                     >  1:1305087/1‑140 (MQ=255)

        cAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTa                                                                                                                                >  1:406354/1‑140 (MQ=255)

         aGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTaa                                                                                                                               <  1:1363133/140‑1 (MQ=255)

          gTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCC‑AATGGTTGATCAAGAGCCTGGAAAGCCGTAACg                                                                                                                             <  2:495704/140‑1 (MQ=255)

           tGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACg                                                                                                                             >  2:830851/1‑140 (MQ=255)

           tGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACg                                                                                                                             <  1:989043/140‑1 (MQ=255)

             aCAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGAt                                                                                                                           >  1:1121226/1‑140 (MQ=255)

              cAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATa                                                                                                                          >  2:37329/1‑140 (MQ=255)

              cAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATa                                                                                                                          <  2:334316/140‑1 (MQ=255)

                gCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGt                                                                                                                                 <  2:795055/131‑1 (MQ=255)

                gCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGt                                                                                                                                 >  1:795055/1‑131 (MQ=255)

                gCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGAt                                                                                                                           <  1:1194959/137‑1 (MQ=255)

                gCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGAt                                                                                                                           >  2:1194959/1‑137 (MQ=255)

                   ttCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTa                                                                                                                     >  1:99156/1‑140 (MQ=255)

                   ttCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTa                                                                                                                     >  1:431015/1‑140 (MQ=255)

                    tCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTAc                                                                                                                    >  2:480597/1‑140 (MQ=255)

                     ccGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACt                                                                                                                   >  2:720915/1‑140 (MQ=255)

                     ccGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCC‑AATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTg                                                                                                                  >  2:1342063/1‑140 (MQ=255)

                      cgcgTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTg                                                                                                                  >  1:370419/1‑140 (MQ=255)

                        cgTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCATGAGCCTGGAAAGCCGt                                                                                                                                 <  1:303182/123‑1 (MQ=255)

                        cgTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCATGAGCCTGGAAAGCCGt                                                                                                                                 >  2:303182/1‑123 (MQ=255)

                         gTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTgcgc                                                                                                               >  2:886361/1‑140 (MQ=255)

                           cTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTaa                                                                                                                               >  1:610304/1‑122 (MQ=255)

                            tGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTg                                                                                                            >  2:694624/1‑140 (MQ=255)

                             gCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGa                                                                                                           >  2:1389035/1‑140 (MQ=255)

                              cAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAg                                                                                                          >  2:725541/1‑140 (MQ=255)

                                 aTCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCg                                                                                                       <  2:1121226/140‑1 (MQ=255)

                                    aaCCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTCTTCCGCGGGAGTCGcag                                                                                                    <  2:953078/140‑3 (MQ=255)

                                    aaCCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTg                                                                                                    <  1:37329/140‑1 (MQ=255)

                                    aaCCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTg                                                                                                    <  2:1146846/140‑1 (MQ=255)

                                    aaCCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTg                                                                                                    <  1:720915/140‑1 (MQ=255)

                                      ccAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTa                                                                                                  <  1:1017033/140‑1 (MQ=255)

                                              aCGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGt                                                                                                         >  2:1262506/1‑125 (MQ=255)

                                              aCGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGt                                                                                                         <  1:1262506/125‑1 (MQ=255)

                                               cGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAAc                                                                                         <  1:1389035/140‑1 (MQ=255)

                                                  cTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGTCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAAcagc                                                                                      >  2:1042764/1‑140 (MQ=255)

                                                  cTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAAcagc                                                                                      <  2:406354/140‑1 (MQ=255)

                                                  cTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAAcagc                                                                                      <  2:431015/140‑1 (MQ=255)

                                                  cTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAAcagc                                                                                      <  2:99156/140‑1 (MQ=255)

                                                    cGATGTGCAGTAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAAcagcag                                                                                    >  2:877626/1‑140 (MQ=255)

                                                    cGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATTGTTGAAcagcag                                                                                    <  1:830851/140‑1 (MQ=255)

                                                    cGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAAcagcag                                                                                    >  1:210416/1‑140 (MQ=255)

                                                    cGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAAcagcag                                                                                    >  1:594093/1‑140 (MQ=255)

                                                      aTGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGt                                                                                                   <  1:874788/123‑1 (MQ=255)

                                                      aTGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGt                                                                                                   >  2:874788/1‑123 (MQ=255)

                                                       tgtgCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTa                                                                                                  <  1:975498/123‑1 (MQ=255)

                                                       tgtgCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTa                                                                                                  >  2:975498/1‑123 (MQ=255)

                                                        gtgCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCC‑AATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGc                                                                               <  1:1342063/140‑1 (MQ=255)

                                                         tgCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGc                                                                               <  1:1392900/140‑1 (MQ=255)

                                                         tgCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGc                                                                               <  1:1068561/140‑1 (MQ=255)

                                                         tgCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCAGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGc                                                                               <  2:232066/140‑1 (MQ=255)

                                                                aCGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCttt                                                                        <  1:886361/140‑1 (MQ=255)

                                                                 cgcgcgCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTg                                                                       >  2:636367/1‑140 (MQ=255)

                                                                 cgcgcgCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTg                                                                       >  1:743549/1‑140 (MQ=255)

                                                                 cgcgcgCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTg                                                                       >  1:1155149/1‑140 (MQ=255)

                                                                   cgcgcAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCTCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAg                                                                     >  2:291043/1‑140 (MQ=255)

                                                                   cgcgcAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAg                                                                     <  2:1155149/140‑1 (MQ=255)

                                                                   cgcgcAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAg                                                                     <  1:966043/140‑1 (MQ=255)

                                                                   cgcgcAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAg                                                                     >  1:319892/1‑140 (MQ=255)

                                                                   cgcgcAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAg                                                                     <  1:1319524/140‑1 (MQ=255)

                                                                    gcgcAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGc                                                                    <  2:594093/140‑1 (MQ=255)

                                                                     cgcAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCa                                                                   <  1:694624/140‑1 (MQ=255)

                                                                     cgcAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCa                                                                   <  2:328603/140‑1 (MQ=255)

                                                                     cgcAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCa                                                                   >  1:328603/1‑140 (MQ=255)

                                                                     cgcAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCa                                                                   <  1:725541/140‑1 (MQ=255)

                                                                     cgcAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCa                                                                   <  2:319892/140‑1 (MQ=255)

                                                                       cAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAg                                                                 <  2:772451/140‑1 (MQ=255)

                                                                          cGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTg                                                              <  2:135193/140‑1 (MQ=255)

                                                                          cGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTg                                                              <  2:370419/140‑1 (MQ=255)

                                                                          cGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTg                                                              <  1:251848/140‑1 (MQ=255)

                                                                          cGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTg                                                              >  2:537745/1‑140 (MQ=255)

                                                                              ggTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTgaaga                                                          <  1:877626/140‑1 (MQ=255)

                                                                              ggTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTgaaga                                                          <  1:995740/140‑1 (MQ=255)

                                                                                  aCAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAg                                                                     <  2:429632/125‑1 (MQ=255)

                                                                                  aCAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAg                                                                     >  1:429632/1‑125 (MQ=255)

                                                                                  aCAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAatat                                                      >  2:508990/1‑140 (MQ=255)

                                                                                     gCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATg                                                   >  2:324312/1‑140 (MQ=255)

                                                                                      ccAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGa                                                  >  1:394720/1‑140 (MQ=255)

                                                                                       cAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGaa                                                 <  2:1305087/140‑1 (MQ=255)

                                                                                         gTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAAc                                               <  1:1438222/140‑1 (MQ=255)

                                                                                         gTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAAc                                               >  1:296752/1‑140 (MQ=255)

                                                                                           ttATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCg                                             <  2:134503/140‑1 (MQ=255)

                                                                                            tATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGa                                            >  2:838316/1‑140 (MQ=255)

                                                                                                   agcaATCTGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACt                                     >  2:660634/1‑140 (MQ=255)

                                                                                                    gcaATCTGTAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACG‑‑‑CTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGcc                                 >  2:103190/1‑140 (MQ=255)

                                                                                                          tGCAGGATGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGat                              <  1:324312/140‑1 (MQ=255)

                                                                                                                 tGCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGccc                       >  1:624886/1‑140 (MQ=255)

                                                                                                                  gCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGat                              <  2:1324587/132‑1 (MQ=255)

                                                                                                                  gCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGat                              >  1:1324587/1‑132 (MQ=255)

                                                                                                                  gCCAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACCCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCa                      >  2:868829/1‑140 (MQ=255)

                                                                                                                    cAAATGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAgg                    <  2:1364968/140‑1 (MQ=255)

                                                                                                                      aaTGGTTGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCt                  >  1:108195/1‑140 (MQ=255)

                                                                                                                           ttGATCAAGAGCCTGGAAAGTCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTGTCGa             <  1:1042764/140‑1 (MQ=255)

                                                                                                                           ttGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTGTCGa             >  2:1325739/1‑140 (MQ=255)

                                                                                                                           ttGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTGTCGa             >  1:990671/1‑140 (MQ=255)

                                                                                                                            tGATCAAGAGCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTGTCGaa            <  1:1123203/140‑1 (MQ=255)

                                                                                                                                  agagCCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTGTCGAAATGCAt      <  1:660634/140‑1 (MQ=255)

                                                                                                                                   gagCCTGGAAAGCCGTAACGATACG‑‑‑CTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTGTCGAAATGCATGAAt  <  1:103190/140‑1 (MQ=255)

GAACTCAACAGTGACAGCATTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGCAGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTATCGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTGTCGAAATGCATGAAT  >  NC_000913/3345578‑3345851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`