Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,592,531	G→A	intergenic (‑206/+193)	ugpB ← / ← livF	sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein/branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,592,531	0	G	A	100.0%	346.5 / NA	98	intergenic (‑206/+193)	ugpB/livF	sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein/branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/54);  total (44/54)

GCTCGAATTACAGAAAAATAACTTTTTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTGAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCACCCAGTAGGCCTGATAAGCGCAGCGC  >  NC_000913/3592394‑3592655                                                                                                                                          |                                                                                                                             gCTCGAATTACAGAAAAATAACTTTTTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTaaa                                                                                                                            >  2:705076/1‑140 (MQ=255) gCTCGAATTACAGAAAAATAACTTTTTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTaaa                                                                                                                            >  2:1049455/1‑140 (MQ=255)  cTCGAATTACAGAAAAATAACTTTTTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAAt                                                                                                                           >  2:76259/1‑140 (MQ=255)  cTCGAATTACAGAAAAATAACTTTTTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAAt                                                                                                                           >  2:79726/1‑140 (MQ=255)     gAATTACAGAAAAATAACTTTTTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCaa                                                                                                                        <  2:201913/140‑1 (MQ=255)       aTTACAGAAAAATAACTTTTTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAAc                                                                                                                      >  1:273229/1‑140 (MQ=255)        ttACAGAAAAATAACTTTTTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAAct                                                                                                                     <  2:1325025/140‑1 (MQ=255)          aCAGAAAAATAACTTTTTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAActct                                                                                                                   <  1:1241531/140‑1 (MQ=255)                 aaTAACTTTTTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGAtt                                                                                                            >  1:1004464/1‑140 (MQ=255)                  aTAACTTTTTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTg                                                                                                           <  2:1081280/140‑1 (MQ=255)                     aCTTTTTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTCCa                                                                                                                <  1:370631/132‑1 (MQ=255)                     aCTTTTTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACa                                                                                                                >  2:370631/1‑132 (MQ=255)                      cTTTCTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTCACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCa                                                                                                       <  1:638107/140‑1 (MQ=255)                                       tAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATg                                                                                      <  1:906870/140‑1 (MQ=255)                                       tAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATg                                                                                      <  2:1004464/140‑1 (MQ=255)                                         aGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATgcg                                                                                    <  2:1427645/140‑1 (MQ=255)                                         aGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATgcg                                                                                    >  2:1429013/1‑140 (MQ=255)                                           aTAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATgcggc                                                                                  >  1:102615/1‑140 (MQ=255)                                               tAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTaa                                                                              >  2:302365/1‑140 (MQ=255)                                               tAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTaa                                                                              >  1:574607/1‑140 (MQ=255)                                               tAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTaa                                                                              >  1:1027041/1‑140 (MQ=255)                                               tAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTa                                                                               <  2:1140807/139‑1 (MQ=255)                                               tAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTa                                                                               >  1:1140807/1‑139 (MQ=255)                                               tAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTa                                                                               <  2:409267/139‑1 (MQ=255)                                               tAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTa                                                                               >  1:409267/1‑139 (MQ=255)                                                   gtgtCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGc                                                                          <  2:955773/140‑1 (MQ=255)                                                    tgtCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGcc                                                                         >  2:1068932/1‑140 (MQ=255)                                                    tgtCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGcc                                                                         >  2:1429904/1‑140 (MQ=255)                                                    tgtCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGcc                                                                         >  2:986854/1‑140 (MQ=255)                                                    tgtCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGcc                                                                         >  2:62222/1‑140 (MQ=255)                                                      tCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTAGAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCtt                                                                       >  2:1359756/1‑140 (MQ=255)                                                      tCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCtt                                                                       >  1:486895/1‑140 (MQ=255)                                                      tCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCtt                                                                       >  2:1462754/1‑140 (MQ=255)                                                      tCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCtt                                                                       >  1:358162/1‑140 (MQ=255)                                                      tCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCtt                                                                       >  2:547518/1‑140 (MQ=255)                                                      tCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCtt                                                                       >  1:676791/1‑140 (MQ=255)                                                      tCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCAGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCtt                                                                       >  1:1285967/1‑140 (MQ=255)                                                      tCAGAAAGATGACAAGGCGGTGACGGCGTGGGGGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCCGCGTAAACGCCtt                                                                       >  2:1461260/1‑140 (MQ=255)                                                        aGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTAt                                                                     <  1:4672/140‑1 (MQ=255)                                                         gAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATc                                                                    >  1:888132/1‑140 (MQ=255)                                                         gAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATc                                                                    <  1:1462754/140‑1 (MQ=255)                                                           aaGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCGCGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  <  1:375589/140‑1 (MQ=255)                                                           aaGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCTGGATGCGGCGTAAACGCCTTATCAg                                                                  <  1:79726/140‑1 (MQ=255)                                                           aaGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  <  1:1359756/140‑1 (MQ=255)                                                           aaGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  <  1:1068932/140‑1 (MQ=255)                                                           aaGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  <  2:486895/140‑1 (MQ=255)                                                           aaGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  <  2:1367231/140‑1 (MQ=255)                                                           aaGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  <  1:602710/140‑1 (MQ=255)                                                           aaGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  <  2:1285967/140‑1 (MQ=255)                                                           aaGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  <  2:1267347/140‑1 (MQ=255)                                                           aaGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  <  2:358162/140‑1 (MQ=255)                                                           aaGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  <  1:986854/140‑1 (MQ=255)                                                           aaGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  <  1:948929/140‑1 (MQ=255)                                                             gATGACAAGGCGGTGGCGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGc                                                                <  2:262284/140‑1 (MQ=255)                                                              aTGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGcc                                                               >  2:496968/1‑140 (MQ=255)                                                              aTGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCCAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGcc                                                               >  1:121680/1‑140 (MQ=255)                                                                gACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTAt                                                                     >  2:875224/1‑132 (MQ=255)                                                                gACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTAt                                                                     <  1:875224/132‑1 (MQ=255)                                                                gACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTa                                                             >  2:35929/1‑140 (MQ=255)                                                                    aGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAg                                                         >  2:1376131/1‑140 (MQ=255)                                                                     ggCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTGTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  >  2:1316072/1‑130 (MQ=255)                                                                     ggCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  <  1:1316072/130‑1 (MQ=255)                                                                     ggCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGa                                                        >  1:77481/1‑140 (MQ=255)                                                                     ggCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGGCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGa                                                        >  2:139010/1‑140 (MQ=255)                                                                         gTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGt                                                    >  2:230808/1‑140 (MQ=255)                                                                         gTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGt                                                    <  1:666528/140‑1 (MQ=255)                                                                           gACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGc                                                  <  1:1094790/140‑1 (MQ=255)                                                                           gACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGc                                                  <  2:574607/140‑1 (MQ=255)                                                                            aCGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  >  1:1036416/1‑123 (MQ=255)                                                                            aCGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAg                                                                  <  2:1036416/123‑1 (MQ=255)                                                                                cGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAAtt                                             <  2:1027041/140‑1 (MQ=255)                                                                                 gTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTc                                            >  1:375974/1‑140 (MQ=255)                                                                                 gTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTc                                            <  1:62222/140‑1 (MQ=255)                                                                                   gggTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCaa                                          <  1:230808/140‑1 (MQ=255)                                                                                   gggTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCaa                                          <  1:705076/140‑1 (MQ=255)                                                                                   gggTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCaa                                          <  2:1238209/140‑1 (MQ=255)                                                                                    ggTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAAc                                         >  2:328544/1‑140 (MQ=255)                                                                                     gTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACa                                        <  2:880488/140‑1 (MQ=255)                                                                                        aGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACatat                                     >  1:604040/1‑140 (MQ=255)                                                                                           gAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGc                                  <  2:888132/140‑1 (MQ=255)                                                                                           gAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGc                                  <  1:723664/140‑1 (MQ=255)                                                                                            aaaaTGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGcc                                 <  1:1058699/140‑1 (MQ=255)                                                                                            aaaaTGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGcc                                 <  1:1429013/140‑1 (MQ=255)                                                                                             aaaTGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCa                                <  1:1429904/140‑1 (MQ=255)                                                                                                 gggAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCa                            <  1:76259/140‑1 (MQ=255)                                                                                                  ggAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCAc                           <  2:77481/140‑1 (MQ=255)                                                                                                    agaTGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCAccc                         >  1:282045/1‑140 (MQ=255)                                                                                                      aTGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCACCCAg                       <  1:328544/140‑1 (MQ=255)                                                                                                      aTGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCACCCAg                       <  2:1066737/140‑1 (MQ=255)                                                                                                      aTGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCACCCAg                       <  2:604040/140‑1 (MQ=255)                                                                                                          ggCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCACCCAGTAgg                   >  2:1385184/1‑140 (MQ=255)                                                                                                           gCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCACCCAGTAGGc                  <  2:198361/140‑1 (MQ=255)                                                                                                           gCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCACCCAGTAGGc                  <  1:207476/140‑1 (MQ=255)                                                                                                           gCACGGATAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCACCCAGTAGGc                  <  1:466303/140‑1 (MQ=255)                                                                                                                 aTAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCAccc                         <  2:1017320/127‑1 (MQ=255)                                                                                                                 aTAAGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCAccc                         >  1:1017320/1‑127 (MQ=255)                                                                                                                   aaGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCACCCAGTAGGc                  >  1:327232/1‑132 (MQ=255)                                                                                                                   aaGCGGGAAAATATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCACCCAGTAGGc                  <  2:327232/132‑1 (MQ=255)                                                                                                                           aaaTATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCACCCAGTAGGCCTGATAagcgcagcgc  >  2:688676/1‑140 (MQ=255)                                                                                                                           aaaTATAGAAGGTCTAAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCACCCAGTAGGCCTGATAagcgcagcgc  >  2:496165/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                             GCTCGAATTACAGAAAAATAACTTTTTTGTTACATTTGTAAGATAGTAAGGTGTCAGAAAGATGACAAGGCGGTGACGGCGTGGGTGAGGGAAAATGGGAGATGGGGCACGGATAAGCGGGAAAATATAGAAGGTCTGAATCAAACTCTACAGATTGCTCATCGTTTCATGCCGGATGCGGCGTAAACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACTCACCCAGTAGGCCTGATAAGCGCAGCGC  >  NC_000913/3592394‑3592655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑