Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,630,072	A→G	S177S (TCT→TCC)	yhiI ←	putative membrane fusion protein YhiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,630,072	0	A	G	100.0%	193.1 / NA	60	S177S (TCT→TCC)	yhiI	putative membrane fusion protein YhiI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/31);  total (29/31)

CACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGCGTATGACGTTTTGCTA  >  NC_000913/3629937‑3630208                                                                                                                                        |                                                                                                                                         cACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAg                                                                                                                                      >  2:1294465/1‑140 (MQ=255) cACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAg                                                                                                                                      >  1:324341/1‑140 (MQ=255) cACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAg                                                                                                                                      >  2:1317802/1‑140 (MQ=255) cACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAg                                                                                                                                      >  2:75992/1‑140 (MQ=255)  aCGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGGTTGCGCCTGAATGATATTGGGGCGTGCCGCCTCTTTAGCCGCTTTGGTAGc                                                                                                                                     >  2:783426/1‑140 (MQ=255)      gtccgtcACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGAt                                                                                                                                 >  1:424518/1‑140 (MQ=255)      gtccgtcACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGAt                                                                                                                                 >  2:415073/1‑140 (MQ=255)         cgtcACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATAcc                                                                                                                              >  1:552564/1‑140 (MQ=255)         cgtcACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGGTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATAcc                                                                                                                              >  2:568396/1‑140 (MQ=255)                cgGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCtt                                                                                                                       <  1:1294465/140‑1 (MQ=255)                cgGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTCTGGAAGCCGATACCTGAGCtt                                                                                                                       <  1:1317802/140‑1 (MQ=255)                     cTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGcc                                                                                                                  <  1:702079/140‑1 (MQ=255)                     cTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGcc                                                                                                                  <  1:846480/140‑1 (MQ=255)                               gCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTTGACGCGGGGTTGCGCCTGAATGATTTTGGTGCGGGCCGCTTTTATAGCCGCTTTGGGAGCCGATACCTGAGCTTTCGCCGATTCCAgcg                                                                                                        >  2:1434346/1‑140 (MQ=255)                                cTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAgcgc                                                                                                       <  1:292356/140‑1 (MQ=255)                                      tCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGGTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCg                                                                                                 >  1:819297/1‑140 (MQ=255)                                      ccGATATCTGCTGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTTGCCGATTCCAGCGCAGCTCg                                                                                                 >  1:781656/2‑140 (MQ=255)                                          tatCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGgcg                                                                                             <  2:552564/140‑1 (MQ=255)                                          tatCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGgcg                                                                                             >  2:226456/1‑140 (MQ=255)                                             cTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGctc                                                                                          >  1:148840/1‑140 (MQ=255)                                             cTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGctc                                                                                          >  1:1299507/1‑140 (MQ=255)                                               gCGGCAATGCGCCGTTCAGTGGCTTGTTCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCTCCGATTCCAGCGCAGCTCGGGCGCtctc                                                                                        >  2:895313/1‑140 (MQ=255)                                               gCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCtctc                                                                                        >  1:777985/1‑140 (MQ=255)                                               gCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCtctc                                                                                        >  2:985973/1‑140 (MQ=255)                                                      tGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAgcggcg                                                                                 <  2:220231/140‑1 (MQ=255)                                                        cgcCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAgcggcggc                                                                               >  2:423078/1‑140 (MQ=255)                                                        cgcCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAgcggcggc                                                                               >  2:1088208/1‑140 (MQ=255)                                                         gcCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAgcggcggcg                                                                              <  1:783426/140‑1 (MQ=255)                                                         gcCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAgcggcggcg                                                                              <  2:777985/140‑1 (MQ=255)                                                          ccGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGgcgc                                                                             <  1:75992/140‑1 (MQ=255)                                                          ccGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGgcgc                                                                             <  2:424518/140‑1 (MQ=255)                                                          ccGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGgcgc                                                                             <  1:902121/140‑1 (MQ=255)                                                                  tGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCa                                                                     >  1:1154233/1‑140 (MQ=255)                                                                       tgtgCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCCGCGGCGGCGCGGTCCGCATCCaa                                                                >  2:1061112/1‑139 (MQ=255)                                                                       tgtgCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAg                                                                <  1:206185/140‑1 (MQ=255)                                                                       tgtgCCGCTTCGACGCGGGGTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAg                                                                >  1:497515/1‑140 (MQ=255)                                                                         tgCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCt                                                              >  2:195513/1‑140 (MQ=255)                                                                          gCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTg                                                             <  1:1061112/140‑1 (MQ=255)                                                                                         gTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGc                                              >  1:438118/1‑140 (MQ=255)                                                                                          tttGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGcc                                             <  1:895313/140‑1 (MQ=255)                                                                                           ttGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGccc                                            <  2:1369757/140‑1 (MQ=255)                                                                                           ttGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGccc                                            <  2:819297/140‑1 (MQ=255)                                                                                           ttGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGccc                                            <  2:1154233/140‑1 (MQ=255)                                                                                           ttGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGccc                                            <  1:985973/140‑1 (MQ=255)                                                                                               gcCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCg                                        <  2:497515/140‑1 (MQ=255)                                                                                                  tGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTg                                     <  1:423078/140‑1 (MQ=255)                                                                                                   gAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTgg                                    >  1:158530/1‑140 (MQ=255)                                                                                                     aTGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGc                                  <  1:1088208/140‑1 (MQ=255)                                                                                                     aTGATATTGGTGCGTGCCGCTTCTATAGCCGCCTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGc                                  <  2:1299507/140‑1 (MQ=255)                                                                                                           ttGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTg                             <  2:108503/139‑1 (MQ=255)                                                                                                           ttGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTg                             >  1:108503/1‑139 (MQ=255)                                                                                                            tGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGaa                           <  2:324341/140‑1 (MQ=255)                                                                                                            tGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGaa                           <  2:593396/140‑1 (MQ=255)                                                                                                            tGGTGCGTGCCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGaa                           <  1:568396/140‑1 (MQ=255)                                                                                                                 cgtgcCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGa                      >  1:355309/1‑140 (MQ=255)                                                                                                                 cgtgcCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGa                      >  1:1377564/1‑140 (MQ=255)                                                                                                                  gtgcCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGaa                     <  2:1403004/140‑1 (MQ=255)                                                                                                                   tgcCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACggg                       <  1:829943/137‑1 (MQ=255)                                                                                                                   tgcCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACggg                       >  2:829943/1‑137 (MQ=255)                                                                                                                   tgcCGCTTCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAAc                    <  2:158530/140‑1 (MQ=255)                                                                                                                         ttCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGCGTAt              >  2:512846/1‑140 (MQ=255)                                                                                                                         ttCTATAGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGAGTAt              >  2:1220053/1‑140 (MQ=255)                                                                                                                             ataGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGCGTATGACg          <  1:286396/140‑1 (MQ=255)                                                                                                                             ataGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGCGTATGACg          >  2:251635/1‑140 (MQ=255)                                                                                                                              taGCCGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGCGTATGACGt         >  1:380809/1‑140 (MQ=255)                                                                                                                                 ccGCTTTGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGCGTATGACGtttt      >  1:238807/1‑140 (MQ=255)                                                                                                                                     tttGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGCGTATGACGTTTTGCTa  >  2:671687/1‑140 (MQ=255)                                                                                                                                     tttGGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGCGAACGGGAACGCGTATGAGGTTTTGCTa  >  2:175150/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         CACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGCGGGTTTGCGCCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCCTCGTTGGGCCAGTGAACGGGAACGCGTATGACGTTTTGCTA  >  NC_000913/3629937‑3630208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑