Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,085,179	T→G	N215T (AAC→ACC)	fdoG ←	formate dehydrogenase O subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,085,179	0	T	G	100.0%	293.6 / NA	84	N215T (AAC→ACC)	fdoG	formate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (34/50);  total (34/50)

AGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGTTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAATTTTTGCGTTAAATAGCCGGTTTCGTTACT  >  NC_000913/4085043‑4085315                                                                                                                                         |                                                                                                                                         aGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAgtgggtgg                                                                                                                                       <  1:1058104/140‑1 (MQ=255) aGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAgtgggtgg                                                                                                                                       >  2:287052/1‑140 (MQ=255)   gatcgatcACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTc                                                                                                                                     >  2:58912/1‑140 (MQ=255)     tcgatcACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCAt                                                                                                                                   <  1:287052/140‑1 (MQ=255)        atcACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGc                                                                                                                                >  1:103274/1‑140 (MQ=255)        atcACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGc                                                                                                                                >  2:955760/1‑140 (MQ=255)           acaATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCAcc                                                                                                                             <  1:993742/140‑1 (MQ=255)            caATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGGCCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACcg                                                                                                                            <  1:958483/140‑1 (MQ=255)            caATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACcg                                                                                                                            <  2:982151/140‑1 (MQ=255)            caATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACcg                                                                                                                            <  2:1355515/140‑1 (MQ=255)            caATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACcg                                                                                                                            <  1:850248/140‑1 (MQ=255)               tCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGa                                                                                                                         >  1:1266029/1‑140 (MQ=255)                   cTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCaa                                                                                                                     <  1:1319530/140‑1 (MQ=255)                   cTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCaa                                                                                                                     >  2:1319530/1‑140 (MQ=255)                   cTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCaa                                                                                                                     <  2:492774/140‑1 (MQ=255)                   cTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCaa                                                                                                                     >  1:581989/1‑140 (MQ=255)                      cgcgCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATg                                                                                                                  >  2:186583/1‑140 (MQ=255)                      cgcgCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATg                                                                                                                  >  2:515962/1‑140 (MQ=255)                      cgcgCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATg                                                                                                                  >  2:620825/1‑140 (MQ=255)                       gcgcCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGa                                                                                                                         <  2:1462206/132‑1 (MQ=255)                       gcgcCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGa                                                                                                                         >  1:1462206/1‑132 (MQ=255)                       gcgcCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGt                                                                                                                 >  2:1327335/1‑140 (MQ=255)                            gTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAg                                                                                                            <  2:1266029/140‑1 (MQ=255)                            gTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAg                                                                                                            <  2:581989/140‑1 (MQ=255)                             ttGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGc                                                                                                           >  1:181874/1‑140 (MQ=255)                                      ttGGCTTGCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGc                                                                                                  <  1:913329/140‑1 (MQ=255)                                      ttGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGc                                                                                                  <  2:181874/140‑1 (MQ=255)                                       tGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGt                                                                                                                 <  2:864047/124‑1 (MQ=255)                                       tGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGt                                                                                                                 >  1:864047/1‑124 (MQ=255)                                       tGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCt                                                                                                 >  2:120088/1‑140 (MQ=255)                                       tGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCt                                                                                                 >  1:449435/1‑140 (MQ=255)                                       tGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCt                                                                                                 >  2:573410/1‑140 (MQ=255)                                           ttCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCg                                                                                             <  1:191448/140‑1 (MQ=255)                                           ttCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCg                                                                                             <  1:1173497/140‑1 (MQ=255)                                            tCCATCGCCCAGCGGAACCCGACCGCGGGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGt                                                                                            >  2:1180385/1‑140 (MQ=255)                                                tCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGt                                                                                        <  2:1266975/140‑1 (MQ=255)                                                tCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGt                                                                                        <  2:449435/140‑1 (MQ=255)                                                tCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGt                                                                                        <  1:573410/140‑1 (MQ=255)                                                 cGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTc                                                                                       <  2:535318/140‑1 (MQ=255)                                                 cGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTc                                                                                       <  1:120088/140‑1 (MQ=255)                                                   cccAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCg                                                                                     >  1:734937/1‑140 (MQ=255)                                                     cAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTc                                                                                       >  1:450321/1‑136 (MQ=255)                                                     cAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTc                                                                                       <  2:450321/136‑1 (MQ=255)                                                            aCCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGaca                                                                            <  2:750062/140‑1 (MQ=255)                                                                  ccGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGacac                                                                           >  1:611541/1‑135 (MQ=255)                                                                  ccGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGacac                                                                           <  2:611541/135‑1 (MQ=255)                                                                  ccGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGcc                                                                      <  2:623455/140‑1 (MQ=255)                                                                  ccGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGcc                                                                      <  1:137220/140‑1 (MQ=255)                                                                  ccGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGcc                                                                      <  2:734937/140‑1 (MQ=255)                                                                  ccGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGcc                                                                      <  1:511922/140‑1 (MQ=255)                                                                        gAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGac                                                                             >  2:1120162/1‑127 (MQ=255)                                                                        gAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGac                                                                             <  1:1120162/127‑1 (MQ=255)                                                                           cTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCg                                                             >  2:1020426/1‑140 (MQ=255)                                                                           cTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCg                                                             >  2:160582/1‑140 (MQ=255)                                                                           cTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCg                                                             <  1:515962/140‑1 (MQ=255)                                                                             tCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGAc                                                           <  1:186583/140‑1 (MQ=255)                                                                             tCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGAc                                                           <  1:91582/140‑1 (MQ=255)                                                                               aGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACcg                                                         <  2:131646/140‑1 (MQ=255)                                                                               aGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACcg                                                         <  1:620825/140‑1 (MQ=255)                                                                               aGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACcg                                                         >  2:700358/1‑140 (MQ=255)                                                                               aGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACcg                                                         <  2:796631/140‑1 (MQ=255)                                                                               aGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACcg                                                         <  2:927590/140‑1 (MQ=255)                                                                               aGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACcg                                                         <  1:160582/140‑1 (MQ=255)                                                                               aGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACcg                                                         <  2:1300938/140‑1 (MQ=255)                                                                                 cggcgTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACcgcg                                                       <  1:58912/140‑1 (MQ=255)                                                                                    cgTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGc                                                    >  2:830791/1‑140 (MQ=255)                                                                                      ttACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGAc                                                           >  2:290615/1‑131 (MQ=255)                                                                                      ttACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGAc                                                           <  1:290615/131‑1 (MQ=255)                                                                                        aCCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACcg                                                         <  1:528848/131‑1 (MQ=255)                                                                                        aCCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACcg                                                         >  2:528848/1‑131 (MQ=255)                                                                                              cATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACcg                                                         <  2:454584/125‑1 (MQ=255)                                                                                              cATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACcg                                                         >  1:454584/1‑125 (MQ=255)                                                                                              cATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAgcg                                          >  1:2881/1‑140 (MQ=255)                                                                                                tCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAgcgcg                                        <  1:263671/140‑1 (MQ=255)                                                                                                 caccacGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAgcgcgc                                       >  2:458912/1‑140 (MQ=255)                                                                                                   ccacGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCgg                                     <  2:1131030/140‑1 (MQ=255)                                                                                                    cacGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCggg                                    >  1:714780/1‑140 (MQ=255)                                                                                                             gTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAAttttt                           <  2:714780/140‑1 (MQ=255)                                                                                                             gTTGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAAttttt                           <  2:1024590/140‑1 (MQ=255)                                                                                                              ttGGCGTTCTTGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAATTTTTg                          >  1:607478/1‑140 (MQ=255)                                                                                                                        tGATGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAATTTTTGCGTTAAATAg                <  1:700358/140‑1 (MQ=255)                                                                                                                          aTGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAATTTTTGCGTTAAATAGcc              <  1:1327335/140‑1 (MQ=255)                                                                                                                           tGTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAATTTTTGCGTTAAATAGCCg             <  1:830791/140‑1 (MQ=255)                                                                                                                            gTCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAATTTTTGCGTTAAATAGCCgg            <  2:1416247/140‑1 (MQ=255)                                                                                                                             tCGACCCAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAATTTTTGCGTTAAATAGCCGGt           >  2:1016927/1‑140 (MQ=255)                                                                                                                                 cccAGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAATTTTTGCGTTAAATAGCCGGTTTCg       >  2:794801/1‑140 (MQ=255)                                                                                                                                    aGTGGGTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAATTTTTGCGTTAAATAGCCGGTTTCGTTa    >  1:927686/1‑140 (MQ=255)                                                                                                                                     gtgggtggTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAATTTTTGCGTTAAATAGCCGGTTTCGTTAc   >  2:475774/1‑140 (MQ=255)                                                                                                                                     gtgggtggTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAATTTTTGCGTTAAATAGCCGGTTTCGTTAc   >  2:443630/1‑140 (MQ=255)                                                                                                                                      tgggtggtCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAATTTTTGCGTTAAATAGCCGGTTTCGTTACt  <  2:201183/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACCCAGTGGTTGGTCATCGCACCGCGACCAAATGTTGGAGCAAGACTTGCTACCGTTGGTCCGTGTCAGACACGCGCCTGGTTGTCGACCGCGAGCATACCCAGCGCGCGGGAGAATTTTTGCGTTAAATAGCCGGTTTCGTTACT  >  NC_000913/4085043‑4085315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑