Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,131,913	A→G	V693V (GTA→GTG)	metL →	fused aspartate kinase/homoserine dehydrogenase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,131,913	0	A	G	100.0%	265.6 / NA	78	V693V (GTA→GTG)	metL	fused aspartate kinase/homoserine dehydrogenase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/38);  total (40/38)

GAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTACGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGTGCTGCGC  >  NC_000913/4131779‑4132045                                                                                                                                       |                                                                                                                                     gAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCgtgt                                                                                                                                 >  2:878384/1‑140 (MQ=255) gAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCgtgt                                                                                                                                 <  1:1323668/140‑1 (MQ=255)   gCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCgtg                                                                                                                                  >  1:1010450/1‑137 (MQ=255)   gCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCgtg                                                                                                                                  <  2:1010450/137‑1 (MQ=255)    cTGGTGGATCGGGCGTGGCAGCAGGGCTTAACCGAACCTGACGCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGTACCGGATCAGGTGCGTGTGGa                                                                                                                              <  2:1045507/140‑1 (MQ=255)    cTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGa                                                                                                                              <  2:1134642/140‑1 (MQ=255)     tggtggATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCTGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGaa                                                                                                                             <  2:1395756/140‑1 (MQ=255)     tggtggATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGaa                                                                                                                             <  1:1396783/140‑1 (MQ=255)     tggtggATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGaa                                                                                                                             >  1:494290/1‑140 (MQ=255)     tggtggATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGaa                                                                                                                             <  1:1163111/140‑1 (MQ=255)              aGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTg                                                                                                                    >  1:482242/1‑140 (MQ=255)              aGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTg                                                                                                                    <  2:78949/140‑1 (MQ=255)                 cGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCt                                                                                                                 <  2:482242/140‑1 (MQ=255)                 cGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCt                                                                                                                 >  1:1090601/1‑140 (MQ=255)                  gTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTg                                                                                                                <  2:1081290/140‑1 (MQ=255)                     gcagcaGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTc                                                                                                             <  2:1090601/140‑1 (MQ=255)                     gcagcaGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTc                                                                                                             >  1:1016406/1‑140 (MQ=255)                      cagcagGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCa                                                                                                            >  1:930929/1‑140 (MQ=255)                           gggCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCg                                                                                                       >  2:638747/1‑140 (MQ=255)                            ggCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGa                                                                                                      >  1:391905/1‑140 (MQ=255)                             gCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGaa                                                                                                     <  2:930929/140‑1 (MQ=255)                                    cGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGgca                                                                                              <  1:638747/140‑1 (MQ=255)                                      aaCCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGgcagc                                                                                            >  2:1139029/1‑140 (MQ=255)                                       aCCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGgcagca                                                                                           >  1:1305469/1‑140 (MQ=255)                                               cgcgTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCAt                                                                                   <  2:834254/140‑1 (MQ=255)                                                 cgTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAggcgg                                                                                                >  2:1385306/1‑125 (MQ=255)                                                 cgTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAggcgg                                                                                                <  1:1385306/125‑1 (MQ=255)                                                 cgTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCAttt                                                                                 >  2:1159596/1‑140 (MQ=255)                                                            ctctGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATg                                                                      <  1:1159596/140‑1 (MQ=255)                                                            ctctGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCAACCATTTCTTTGAAAATg                                                                      >  1:1408632/1‑140 (MQ=255)                                                                  cAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGAtg                                                                >  1:1390653/1‑140 (MQ=255)                                                                  cAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGAtg                                                                >  2:1020936/1‑140 (MQ=255)                                                                     aGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGAtgaac                                                             >  2:1122088/1‑140 (MQ=255)                                                                       aCGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCg                                                                   <  1:1084163/132‑1 (MQ=255)                                                                       aCGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCg                                                                   >  2:1084163/1‑132 (MQ=255)                                                                       aCGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGAtgaactg                                                           >  1:1115428/1‑140 (MQ=255)                                                                       aCGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGAtgaactg                                                           >  2:1421310/1‑140 (MQ=255)                                                                           gATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACg                                                       >  1:677121/1‑140 (MQ=255)                                                                            aTGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGa                                                      <  2:1408632/140‑1 (MQ=255)                                                                                 cAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGa                                                 <  1:548729/140‑1 (MQ=255)                                                                                 cAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGa                                                 >  2:410393/1‑140 (MQ=255)                                                                                 cAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGa                                                 >  2:548729/1‑140 (MQ=255)                                                                                      tGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTg                                            <  2:634489/140‑1 (MQ=255)                                                                                       ggTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGc                                           >  1:344554/1‑140 (MQ=255)                                                                                              cTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAg                                                  >  1:775371/1‑126 (MQ=255)                                                                                              cTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAg                                                  <  2:775371/126‑1 (MQ=255)                                                                                              cTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCt                                    >  1:391683/1‑140 (MQ=255)                                                                                                ggCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCa                                                   <  1:1091978/123‑1 (MQ=255)                                                                                                ggCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGGGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCa                                                   >  2:1091978/1‑123 (MQ=255)                                                                                                  cgcgTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCaa                                         >  2:1226276/1‑131 (MQ=255)                                                                                                  cgcgTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCaa                                         <  1:1226276/131‑1 (MQ=255)                                                                                                  cgcgTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGaa                                >  2:510553/1‑140 (MQ=255)                                                                                                  cgcgTGAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGaa                                >  1:1117544/1‑140 (MQ=255)                                                                                                       gAAGCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGc                           <  1:1122088/140‑1 (MQ=255)                                                                                                          gCAGGTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCcg                        <  1:193273/140‑1 (MQ=255)                                                                                                             ggTTACAACATCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGa                     <  1:410393/140‑1 (MQ=255)                                                                                                                  caacaTCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATgg                >  1:151935/1‑140 (MQ=255)                                                                                                                     caTCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGc             <  2:677121/140‑1 (MQ=255)                                                                                                                     caTCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGc             <  2:1336703/140‑1 (MQ=255)                                                                                                                     caTCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGc             <  1:260121/140‑1 (MQ=255)                                                                                                                       tCGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTg           <  2:1305469/140‑1 (MQ=255)                                                                                                                        cGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTgg          <  2:151935/140‑1 (MQ=255)                                                                                                                        cGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTgg          >  2:586218/1‑140 (MQ=255)                                                                                                                        cGAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTgg          >  2:806263/1‑140 (MQ=255)                                                                                                                         gAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGc             <  2:145130/136‑1 (MQ=255)                                                                                                                         gAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGc             <  2:143655/136‑1 (MQ=255)                                                                                                                         gAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGc             >  1:145130/1‑136 (MQ=255)                                                                                                                         gAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGc             >  1:143655/1‑136 (MQ=255)                                                                                                                         gAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGt         >  2:1419252/1‑140 (MQ=255)                                                                                                                         gAACCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGt         >  1:234092/1‑140 (MQ=255)                                                                                                                          aaCCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGtg        <  2:494290/140‑1 (MQ=255)                                                                                                                          aaCCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGtg        <  1:586218/140‑1 (MQ=255)                                                                                                                          aaCCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGtg        <  2:391905/140‑1 (MQ=255)                                                                                                                          aaCCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGtg        >  2:201556/1‑140 (MQ=255)                                                                                                                          aaCCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGtg        <  1:1421310/140‑1 (MQ=255)                                                                                                                          aaCCGGATCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGtg        >  2:806123/1‑140 (MQ=255)                                                                                                                                aTCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGTGCTgcgc  >  2:279594/1‑140 (MQ=255)                                                                                                                                aTCAGGTGCGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGTGCTgcgc  <  2:1115428/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     GAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTACGTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAGCAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGTGCTGCGC  >  NC_000913/4131779‑4132045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑