Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,162,148	G→A	intergenic (+18/+22)	yijD → / ← trmA	conserved inner membrane protein YijD/tRNA m(5)U54 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,162,148	0	G	A	100.0%	335.0 / NA	95	intergenic (+18/+22)	yijD/trmA	conserved inner membrane protein YijD/tRNA m(5)U54 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/51);  total (44/51)

TTCGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCAT  >  NC_000913/4162015‑4162284                                                                                                                                      |                                                                                                                                          ttCGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGgcg                                                                                                                                     >  2:688670/1‑140 (MQ=255)   cGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCa                                                                                                                                   >  1:875305/1‑140 (MQ=255)   cGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCa                                                                                                                                   <  2:258877/140‑1 (MQ=255)   cGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCa                                                                                                                                   >  1:912947/1‑140 (MQ=255)   cGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCATAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCa                                                                                                                                   >  2:1118812/1‑140 (MQ=255)     tGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCATAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATc                                                                                                                                 <  1:1118812/140‑1 (MQ=255)       cGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCg                                                                                                                               >  2:1210753/1‑140 (MQ=255)        ggAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCgg                                                                                                                              >  2:664677/1‑140 (MQ=255)         gAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGc                                                                                                                             <  1:323828/140‑1 (MQ=255)          aaTATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCa                                                                                                                            <  2:1012936/140‑1 (MQ=255)               ccGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCAt                                                                                                                           >  1:494168/1‑136 (MQ=255)               ccGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCAt                                                                                                                           <  2:494168/136‑1 (MQ=255)                   atatCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTa                                                                                                                   >  1:875548/1‑140 (MQ=255)                    tatCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTAc                                                                                                                  >  2:958311/1‑140 (MQ=255)                    tatCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTAc                                                                                                                  >  1:834920/1‑140 (MQ=255)                     atCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACt                                                                                                                 <  2:518198/140‑1 (MQ=255)                      tCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACtt                                                                                                                >  2:685834/1‑140 (MQ=255)                        ggCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTcg                                                                                                              <  2:1151362/140‑1 (MQ=255)                        ggCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTcg                                                                                                              <  1:789896/140‑1 (MQ=255)                         gCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTcgc                                                                                                             >  1:1339879/1‑140 (MQ=255)                          ctctAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTcgcg                                                                                                            >  2:595714/1‑140 (MQ=255)                          ctctAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTcgcg                                                                                                            <  2:1086573/140‑1 (MQ=255)                          ctctAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTcgcg                                                                                                            >  1:584072/1‑140 (MQ=255)                            ctAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGt                                                                                                          <  1:1331224/140‑1 (MQ=255)                             tAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTc                                                                                                         >  1:922624/1‑140 (MQ=255)                             tAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTc                                                                                                         >  2:371738/1‑140 (MQ=255)                               aCTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAg                                                                                                       >  2:902165/1‑140 (MQ=255)                                      cccGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATAcg                                                                                                <  2:834920/140‑1 (MQ=255)                                      cccGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATAcg                                                                                                <  1:902165/140‑1 (MQ=255)                                         ggCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATAcgccg                                                                                             >  1:921929/1‑140 (MQ=255)                                          gCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATAcgccgc                                                                                            <  2:110167/140‑1 (MQ=255)                                          gCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATAcgccgc                                                                                            <  2:922624/140‑1 (MQ=255)                                          gCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATAcgccgc                                                                                            <  1:685834/140‑1 (MQ=255)                                          gCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATAcgccgc                                                                                            <  1:122355/140‑1 (MQ=255)                                          gCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATAcgccac                                                                                            <  1:866022/140‑3 (MQ=255)                                           cAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCC‑GATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAc                                                                                          <  2:1430692/140‑1 (MQ=255)                                            aGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAc                                                                                          <  2:1246950/140‑1 (MQ=255)                                            aGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAc                                                                                          <  2:912947/140‑1 (MQ=255)                                                ctctCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCa                                                                                     >  1:71087/1‑140 (MQ=255)                                                   tcCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCatat                                                                                  >  2:72107/1‑140 (MQ=255)                                                   tcCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCatat                                                                                  >  2:1438595/1‑140 (MQ=255)                                                    ccGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAc                                                                                          <  1:762100/132‑1 (MQ=255)                                                    ccGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAc                                                                                          >  2:762100/1‑132 (MQ=255)                                                     cGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCa                                                                                           >  2:73689/1‑130 (MQ=255)                                                     cGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCa                                                                                           <  1:73689/130‑1 (MQ=255)                                                      gTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGt                                                                               <  1:880660/140‑1 (MQ=255)                                                      gTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGt                                                                               <  1:1210753/140‑1 (MQ=255)                                                        cATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGc                                                                             >  1:951767/1‑140 (MQ=255)                                                        cATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGc                                                                             >  1:1122115/1‑140 (MQ=255)                                                         aTTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCg                                                                            <  1:1098931/140‑1 (MQ=255)                                                         aTTATGGTGTTCTGGATTGGCACGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCg                                                                            <  2:1339879/140‑1 (MQ=255)                                                                  ttCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGa                                                                   >  1:177643/1‑140 (MQ=255)                                                                  ttCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGa                                                                   <  1:158292/140‑1 (MQ=255)                                                                  ttCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGa                                                                   >  1:277459/1‑140 (MQ=255)                                                                  ttCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGa                                                                   <  1:72107/140‑1 (MQ=255)                                                                  ttCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGa                                                                   <  2:944573/140‑1 (MQ=255)                                                                    cTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGt                                                                               <  2:611172/126‑1 (MQ=255)                                                                    cTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGt                                                                               <  2:35347/126‑1 (MQ=255)                                                                    cTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGt                                                                               >  1:611172/1‑126 (MQ=255)                                                                    cTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGt                                                                               >  1:35347/1‑126 (MQ=255)                                                                    cTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAAc                                                                 >  2:157991/1‑140 (MQ=255)                                                                    cTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAAc                                                                 <  2:1122115/140‑1 (MQ=255)                                                                     tGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGt                                                                               >  2:1430145/1‑125 (MQ=255)                                                                     tGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGt                                                                               <  1:1430145/125‑1 (MQ=255)                                                                     tGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACt                                                                >  2:1259248/1‑140 (MQ=255)                                                                            gcgcGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGa                                                              >  1:410891/1‑135 (MQ=255)                                                                            gcgcGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGa                                                              <  2:410891/135‑1 (MQ=255)                                                                            gcgcGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCaaa                                                         <  1:157991/140‑1 (MQ=255)                                                                            gcgcGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCaaa                                                         >  1:29065/1‑140 (MQ=255)                                                                                   gATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAATAGAGcc                                                  <  2:921929/140‑1 (MQ=255)                                                                                       cGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGAc                                              >  2:1426597/1‑140 (MQ=255)                                                                                       cGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGAc                                              >  2:1227528/1‑140 (MQ=255)                                                                                          aaCCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGtt                                           <  1:1426597/140‑1 (MQ=255)                                                                                            ccGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAg                                                >  1:866318/1‑133 (MQ=255)                                                                                            ccGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAg                                                <  2:866318/133‑1 (MQ=255)                                                                                            ccGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCg                                         <  2:584072/140‑1 (MQ=255)                                                                                             cGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGa                                        <  1:688670/140‑1 (MQ=255)                                                                                               tAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAg                                                >  1:1060369/1‑130 (MQ=255)                                                                                               tAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAg                                                <  2:1060369/130‑1 (MQ=255)                                                                                                 aGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCtt                                    >  1:351231/1‑140 (MQ=255)                                                                                                      gAAGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCg                               >  2:1354465/1‑140 (MQ=255)                                                                                                       aaGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGt                              <  2:445291/140‑1 (MQ=255)                                                                                                       aaGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGt                              <  2:351231/140‑1 (MQ=255)                                                                                                       aaGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGt                              <  2:189835/140‑1 (MQ=255)                                                                                                       aaGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGt                              <  2:875305/140‑1 (MQ=255)                                                                                                       aaGTTGCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGt                              <  1:371738/140‑1 (MQ=255)                                                                                                            gCTGAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGc                         <  2:29065/140‑1 (MQ=255)                                                                                                               gAGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCCTGTGCGTCTGGCTTa                      <  1:595714/140‑1 (MQ=255)                                                                                                                aGTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTaa                     <  2:71087/140‑1 (MQ=255)                                                                                                                 gTAATCGGTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAAt                    <  2:177643/140‑1 (MQ=255)                                                                                                                        gTATTATGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCa             <  2:898338/140‑1 (MQ=255)                                                                                                                          attatGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCACTTCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAg            >  1:1021543/1‑140 (MQ=255)                                                                                                                              tGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCt       >  1:543928/1‑140 (MQ=255)                                                                                                                              tGCCGGATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCt       >  2:43403/1‑140 (MQ=255)                                                                                                                                   gATACTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCAt  >  2:1067182/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                          TTCGTGCGGAATATCCGGATATCGGCTCTAACTTCTTCCCGGCAGTACTCTCCGTCATTATGGTGTTCTGGATTGGCGCGAAGATGCGTAACCGTAAGCAGGAAGTTGCTGAGTAATCGGTATTATGCCGGATGCTGGCGCATCCGGCATGGGTTTTACTTCGCGGTCAGTAATACGCCGCAC‑TCCATATGGTGCGTGTAGGGGAACTGATCAAACAGAGCCAGACGTTCGACCTTGTGCGTCTGGCTTAATGTTTCCAGATTCTTGCAT  >  NC_000913/4162015‑4162284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑