Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,370,000	G→A	T138I (ACT→ATT)	yjeH ←	L‑methionine/branched chain amino acid exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,370,000	0	G	A	100.0%	266.5 / NA	76	T138I (ACT→ATT)	yjeH	L‑methionine/branched chain amino acid exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/37);  total (39/37)

AAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAA  >  NC_000913/4369864‑4370136                                                                                                                                         |                                                                                                                                         aaGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATAc                                                                                                                                       <  2:467199/140‑1 (MQ=255)   gTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCg                                                                                                                                     >  1:312202/1‑140 (MQ=255)    ttCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGa                                                                                                                                    <  2:987600/140‑1 (MQ=255)    ttCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGa                                                                                                                                    <  2:1172341/140‑1 (MQ=255)    ttCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGa                                                                                                                                    <  1:453834/140‑1 (MQ=255)    ttCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGa                                                                                                                                    <  1:372807/140‑1 (MQ=255)    ttCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGa                                                                                                                                    <  2:872394/140‑1 (MQ=255)              ccAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCacac                                                                                                                          >  2:1091682/1‑140 (MQ=255)              ccAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCacac                                                                                                                          <  2:1108054/140‑1 (MQ=255)                 ggTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACAccag                                                                                                                       <  1:906708/140‑1 (MQ=255)                  gTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGa                                                                                                                                    <  2:492078/126‑1 (MQ=255)                  gTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGa                                                                                                                                    >  1:492078/1‑126 (MQ=255)                   tGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACAccagcg                                                                                                                     <  2:613239/140‑1 (MQ=255)                   tGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACAccagcg                                                                                                                     <  1:453593/140‑1 (MQ=255)                   tGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACAccagcg                                                                                                                     >  2:453593/1‑140 (MQ=255)                     ccGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACAccagcgcc                                                                                                                   >  2:531431/1‑140 (MQ=255)                      cGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACAccagcgcca                                                                                                                  >  2:514906/1‑140 (MQ=255)                      cGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACAccagcgcca                                                                                                                  <  1:514906/140‑1 (MQ=255)                      cGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACAccagcgcca                                                                                                                  >  2:877414/1‑140 (MQ=255)                        gAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACAccagcgccagc                                                                                                                <  1:143054/140‑1 (MQ=255)                            ggggATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTAc                                                                                                            <  2:1052038/140‑1 (MQ=255)                               gATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGa                                                                                                         >  1:266412/1‑140 (MQ=255)                                atatTCGCAGGTTTGATATCGTCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAg                                                                                                        >  2:535832/1‑140 (MQ=255)                                         ggTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCcaa                                                                                               <  1:697488/140‑1 (MQ=255)                                          gTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCcaac                                                                                              >  2:1423705/1‑140 (MQ=255)                                             tGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCaacaac                                                                                           >  2:865530/1‑140 (MQ=255)                                             tGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGACACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCaacaac                                                                                           <  1:260819/140‑1 (MQ=255)                                               atatCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAg                                                                                         >  2:254345/1‑140 (MQ=255)                                                 atCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGtt                                                                                       <  2:47041/140‑1 (MQ=255)                                                  tCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAATTCTGCCAACAACAGTTg                                                                                      <  1:1091682/140‑1 (MQ=255)                                                    gcccgcccACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGcc                                                                                    >  1:684622/1‑140 (MQ=255)                                                     cccgcccACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCa                                                                                   >  1:1317349/1‑140 (MQ=255)                                                     cccgcccACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCa                                                                                   >  2:420360/1‑140 (MQ=255)                                                     cccgcccACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCa                                                                                   >  2:1084089/1‑140 (MQ=255)                                                     cccgcccACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCa                                                                                   >  2:219558/1‑140 (MQ=255)                                                     cccgcccACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCa                                                                                   >  1:308512/1‑140 (MQ=255)                                                      ccgcccACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAg                                                                                  <  1:877414/140‑1 (MQ=255)                                                       cgcccACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGc                                                                                 <  1:531431/140‑1 (MQ=255)                                                         cccACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATGACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCTGACAACAGTTGCCAGCTa                                                                               >  2:1310015/1‑140 (MQ=255)                                                         cccACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTa                                                                               >  1:90863/1‑140 (MQ=255)                                                         cccACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTa                                                                               >  1:451729/1‑140 (MQ=255)                                                            accaGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATgc                                                                            <  1:1423705/140‑1 (MQ=255)                                                            accaGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATgc                                                                            <  1:254345/140‑1 (MQ=255)                                                                gATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTAGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATgccagc                                                                        >  2:1310290/1‑140 (MQ=255)                                                                     cGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAAc                                                                   >  1:756990/1‑140 (MQ=255)                                                                     cGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAAATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAAc                                                                   >  2:685570/1‑140 (MQ=255)                                                                      gACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAAATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACa                                                                  <  1:685570/140‑1 (MQ=255)                                                                          aTCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGc                                                              <  2:756990/140‑1 (MQ=255)                                                                          aTCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGc                                                              >  1:200897/1‑140 (MQ=255)                                                                          aTCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGc                                                              >  2:316973/1‑140 (MQ=255)                                                                           tCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGcc                                                             >  1:997366/1‑140 (MQ=255)                                                                           tCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGcc                                                             >  2:170814/1‑140 (MQ=255)                                                                               cgcgACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGc                                                         >  1:549166/1‑140 (MQ=255)                                                                                   acgaTAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCg                                                               <  2:1201624/130‑1 (MQ=255)                                                                                   acgaTAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCg                                                               >  1:1201624/1‑130 (MQ=255)                                                                                    cgaTAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAAc                                                    >  2:1092315/1‑140 (MQ=255)                                                                                        aaGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCcgg                                                <  1:170814/140‑1 (MQ=255)                                                                                         aGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCcggc                                               <  2:308512/140‑1 (MQ=255)                                                                                          gTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCcggcg                                              <  2:684622/140‑1 (MQ=255)                                                                                              ggCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCaa                                          >  1:831431/1‑140 (MQ=255)                                                                                                       ggTTTGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTg                                 <  1:865530/140‑1 (MQ=255)                                                                                                         tttGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCg                               <  1:1084089/140‑1 (MQ=255)                                                                                                          ttGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCgg                              <  1:316973/140‑1 (MQ=255)                                                                                                           tGTAGATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCggc                             >  2:850181/1‑140 (MQ=255)                                                                                                               gATTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCTAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCggcaggc                         <  1:1310015/140‑1 (MQ=255)                                                                                                                aTTAGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCggcaggca                        <  1:219558/140‑1 (MQ=255)                                                                                                                   aGCACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAAc                     >  1:631114/1‑140 (MQ=255)                                                                                                                     cACTGGAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAAccc                   <  2:200897/140‑1 (MQ=255)                                                                                                                          gAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACggg              >  1:760882/1‑140 (MQ=255)                                                                                                                          gAACTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACggg              >  1:1329009/1‑140 (MQ=255)                                                                                                                           aaCTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCggcaggca                        >  1:248754/1‑129 (MQ=255)                                                                                                                           aaCTGGCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCggcaggca                        <  2:248754/129‑1 (MQ=255)                                                                                                                               ggCACCGCGAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGa         >  2:1187616/1‑140 (MQ=255)                                                                                                                                gCACCGCGAATA‑CGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGa         <  1:1024050/138‑1 (MQ=255)                                                                                                                                gCACCGCGAATA‑CGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGa         >  2:1024050/1‑138 (MQ=255)                                                                                                                                      cgAATACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATaa  <  1:1092315/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAA  >  NC_000913/4369864‑4370136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑