Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,396,129	C→T	A22V (GCC→GTC)	amiB →	N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,396,129	0	C	T	100.0%	290.3 / NA	83	A22V (GCC→GTC)	amiB	N‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/46);  total (37/46)

GTGAGTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGCCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGC  >  NC_000913/4395993‑4396265                                                                                                                                         |                                                                                                                                         gTGAGTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTcgc                                                                                                                                       >  2:183133/1‑140 (MQ=255)  tGAGTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTcgcg                                                                                                                                      <  2:31781/140‑1 (MQ=255)   gAGTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGa                                                                                                                                     <  2:752813/140‑1 (MQ=255)    aGTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGAc                                                                                                                                    <  2:383708/140‑1 (MQ=255)     gtgtGGTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACg                                                                                                                                   <  2:899604/140‑1 (MQ=255)     gTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACg                                                                                                                                   <  2:471194/140‑1 (MQ=255)     gTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACg                                                                                                                                   <  2:1460246/140‑1 (MQ=255)     gTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACg                                                                                                                                   <  1:1240084/140‑1 (MQ=255)     gTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACg                                                                                                                                   <  1:1266886/140‑1 (MQ=255)     gTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACg                                                                                                                                   <  2:792925/140‑1 (MQ=255)     gTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACg                                                                                                                                   <  1:133710/140‑1 (MQ=255)       gCGGTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGct                                                                                                                                 <  2:880496/140‑1 (MQ=255)         ggTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGctct                                                                                                                               <  2:1258838/140‑1 (MQ=255)          gTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGctctc                                                                                                                              <  1:832724/140‑1 (MQ=255)          gTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGctctc                                                                                                                              <  1:736009/140‑1 (MQ=255)          gTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGctctc                                                                                                                              <  1:275542/140‑1 (MQ=255)           tttCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGctctct                                                                                                                             >  1:430843/1‑140 (MQ=255)           tttCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGctctc                                                                                                                              >  1:525664/1‑139 (MQ=255)           tttCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGctctc                                                                                                                              <  2:525664/139‑1 (MQ=255)             tCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTACTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGa                                                                                                                           >  1:486815/1‑140 (MQ=255)                  tGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTc                                                                                                                      >  1:175802/1‑140 (MQ=255)                    cGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAg                                                                                                                    <  1:1145440/140‑1 (MQ=255)                    cGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAg                                                                                                                    >  2:93158/1‑140 (MQ=255)                    cGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTACTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAg                                                                                                                    <  2:486815/140‑1 (MQ=255)                     gggTGAATTGATGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAgg                                                                                                                   <  1:1372654/140‑1 (MQ=255)                          aaTTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCt                                                                                                              <  2:611053/140‑1 (MQ=255)                            ttgttgCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTaa                                                                                                            <  1:341128/140‑1 (MQ=255)                            ttgttgCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTaa                                                                                                            <  1:93158/140‑1 (MQ=255)                                 gCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTa                                                                                                             <  2:913271/134‑1 (MQ=255)                                 gCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTa                                                                                                             >  1:913271/1‑134 (MQ=255)                                     gcgcGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCa                                                                                                   >  2:1225240/1‑140 (MQ=255)                                     gcgcGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCa                                                                                                   >  2:971385/1‑140 (MQ=255)                                      cgcgTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATGTTCAGGTTTCTAACGGTAATCaa                                                                                                  <  2:274357/140‑1 (MQ=255)                                      cgcgTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCaa                                                                                                  <  1:55939/140‑1 (MQ=255)                                       gcgTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTaa                                                                                                            <  2:915144/129‑1 (MQ=255)                                       gcgTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTaa                                                                                                            >  1:915144/1‑129 (MQ=255)                                            tAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGgcg                                                                                            >  1:561394/1‑140 (MQ=255)                                               ccGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGgc                                                                                             >  2:952317/1‑136 (MQ=255)                                               ccGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGgc                                                                                             <  1:952317/136‑1 (MQ=255)                                                cGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTACACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGa                                                                                        <  2:1292592/140‑1 (MQ=255)                                                          ttGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAg                                                                              <  2:236619/140‑1 (MQ=255)                                                            gAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGtt                                                                            >  1:500653/1‑140 (MQ=255)                                                                  tGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTg                                                                      >  2:847018/1‑140 (MQ=255)                                                                    gCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGc                                                                    <  2:1422399/140‑1 (MQ=255)                                                                      gggATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGa                                                                  <  1:971385/140‑1 (MQ=255)                                                                      gggATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGa                                                                  <  1:1444466/140‑1 (MQ=255)                                                                      gggATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGa                                                                  <  1:1225240/140‑1 (MQ=255)                                                                       ggATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGAt                                                                 >  2:547903/1‑140 (MQ=255)                                                                            atgTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGa                                                            >  1:1313639/1‑140 (MQ=255)                                                                               tATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTa                                                         <  1:183133/140‑1 (MQ=255)                                                                               tATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTa                                                         <  1:847018/140‑1 (MQ=255)                                                                                   gCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCg                                                     >  2:361576/1‑140 (MQ=255)                                                                                    cATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACTGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTgg                                                                     <  2:737290/123‑1 (MQ=255)                                                                                    cATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACTGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTgg                                                                     >  1:737290/1‑123 (MQ=255)                                                                                      tCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGttt                                                  >  2:222516/1‑140 (MQ=255)                                                                                      tCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGttt                                                  >  1:1001840/1‑140 (MQ=255)                                                                                       cAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTa                                                 >  1:445700/1‑140 (MQ=255)                                                                                         gAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGc                                               >  2:542912/1‑140 (MQ=255)                                                                                                  tggtAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGc                                      <  2:1001840/140‑1 (MQ=255)                                                                                                    gtAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATc                                           <  2:658105/133‑1 (MQ=255)                                                                                                    gtAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATc                                           >  1:658105/1‑133 (MQ=255)                                                                                                    gtAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCaa                                    >  1:1014092/1‑140 (MQ=255)                                                                                                     tAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCaaa                                   >  1:530005/1‑140 (MQ=255)                                                                                                     tAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCaaa                                   <  2:445700/140‑1 (MQ=255)                                                                                                         gACGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCa                               <  1:790911/140‑1 (MQ=255)                                                                                                          aCGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCaa                                         >  1:1086564/1‑129 (MQ=255)                                                                                                          aCGCTGCTGCTGCTGTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCaa                                         <  2:1086564/129‑1 (MQ=255)                                                                                                              tgctgctgctgTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTg                          <  1:1373528/140‑1 (MQ=255)                                                                                                                   ctgctgTGCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCt                     <  2:430843/140‑1 (MQ=255)                                                                                                                        gtgCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGata                >  2:498241/1‑140 (MQ=255)                                                                                                                        gtgCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGata                >  1:1037527/1‑140 (MQ=255)                                                                                                                         tgCACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGatat               >  1:131304/1‑140 (MQ=255)                                                                                                                           cACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCt                     >  1:686162/1‑132 (MQ=255)                                                                                                                           cACGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCt                     <  2:686162/132‑1 (MQ=255)                                                                                                                             cGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATcaaa           >  2:1081934/1‑140 (MQ=255)                                                                                                                             cGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATcaaa           >  2:155630/1‑140 (MQ=255)                                                                                                                             cGCCGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATcaaa           >  2:1244292/1‑140 (MQ=255)                                                                                                                                cGGTGGGTGTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATcaaacaa        >  1:242329/1‑140 (MQ=255)                                                                                                                                 ggtgggtgTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATcaaacaaa       <  1:1216135/140‑1 (MQ=255)                                                                                                                                   tgggtgTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACg     >  1:436013/1‑140 (MQ=255)                                                                                                                                     ggtgTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACggg   >  2:185556/1‑140 (MQ=255)                                                                                                                                     ggtgTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACg     >  2:635949/1‑138 (MQ=255)                                                                                                                                     ggtgTCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACg     <  1:635949/138‑1 (MQ=255)                                                                                                                                      gtgtCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGc  <  1:1286635/140‑1 (MQ=255)                                                                                                                                      gtgtCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGc  <  2:242329/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GTGAGTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGGCGCGTTTAGCCGGTTAACCTTTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGCTGCTGCTGCTGTGCACGCCGGTGGGTGCCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGC  >  NC_000913/4395993‑4396265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑