Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,549,854	A→G	intergenic (+144/+99)	fimH → / ← gntP	type 1 fimbriae D‑mannose specific adhesin/fructuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,549,854	0	A	G	100.0%	266.7 / NA	77	intergenic (+144/+99)	fimH/gntP	type 1 fimbriae D‑mannose specific adhesin/fructuronate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/39);  total (38/39)

ACATTGCTAATGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCA  >  NC_000913/4549723‑4549987                                                                                                                                    |                                                                                                                                      aCATTGCTAATGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAAcc                                                                                                                               <  2:156423/140‑1 (MQ=255)   aTTGCTAATGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCct                                                                                                                             >  2:287223/1‑140 (MQ=255)     tGCTAATGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCctct                                                                                                                           >  1:522022/1‑140 (MQ=255)          aTGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCg                                                                                                                      >  2:95557/1‑140 (MQ=255)          aTGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCg                                                                                                                      >  2:1273257/1‑140 (MQ=255)          aTGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCg                                                                                                                      >  1:383121/1‑140 (MQ=255)          aTGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCg                                                                                                                      >  1:272907/1‑140 (MQ=255)           tGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCgg                                                                                                                     >  2:875362/1‑140 (MQ=255)            gCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGa                                                                                                                    >  1:625113/1‑140 (MQ=255)            gCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGa                                                                                                                    >  1:1356341/1‑140 (MQ=255)            gCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGa                                                                                                                    >  2:1413200/1‑140 (MQ=255)              tGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAgg                                                                                                                  <  1:1145461/140‑1 (MQ=255)              tGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAgg                                                                                                                  <  1:862569/140‑1 (MQ=255)              tGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAgg                                                                                                                  <  2:383121/140‑1 (MQ=255)               ggTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAggg                                                                                                                 >  1:810439/1‑140 (MQ=255)                      aaTATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTcc                                                                                                                         <  2:771001/125‑1 (MQ=255)                      aaTATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTcc                                                                                                                         >  1:771001/1‑125 (MQ=255)                       atatTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAgggg                                                                                                         >  2:1055237/1‑140 (MQ=255)                        tatTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGa                                                                                                        >  1:1238523/1‑140 (MQ=255)                        tatTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGa                                                                                                        >  2:244134/1‑140 (MQ=255)                        tatTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGa                                                                                                        >  1:766709/1‑140 (MQ=255)                         atTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGAc                                                                                                       <  2:1356341/140‑1 (MQ=255)                           tACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTg                                                                                                     >  2:738995/1‑140 (MQ=255)                                 aaGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCggg                                                                                               <  2:955358/140‑1 (MQ=255)                                 aaGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCggg                                                                                               <  1:811595/140‑1 (MQ=255)                                 aaGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCggg                                                                                               <  2:810439/140‑1 (MQ=255)                                  aGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGc                                                                                              >  2:472151/1‑140 (MQ=255)                                  aGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGc                                                                                              >  1:127841/1‑140 (MQ=255)                                  aGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGc                                                                                              >  1:787888/1‑140 (MQ=255)                                  aGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGc                                                                                              >  1:1215611/1‑140 (MQ=255)                                  aGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCCCCCCCCGGAGGGGCGAGGGGCCTGCCCggcc                                                                                              >  1:1204445/1‑138 (MQ=37)                                   gCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGca                                                                                             <  2:1238523/140‑1 (MQ=255)                                   gCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGca                                                                                             <  2:104945/140‑1 (MQ=255)                                   gCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGca                                                                                             <  2:766709/140‑1 (MQ=255)                                          aCCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACAttttt                                                                                      <  2:1215611/140‑1 (MQ=255)                                            cTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAg                                                                                    <  1:263159/140‑1 (MQ=255)                                            cTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAg                                                                                    <  2:715164/140‑1 (MQ=255)                                            cTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAg                                                                                    <  1:563825/140‑1 (MQ=255)                                                      gCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGtcat                                                                          <  2:1006092/140‑1 (MQ=255)                                                      gCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGtcat                                                                          >  2:786378/1‑140 (MQ=255)                                                      gCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGtcat                                                                          <  2:625113/140‑1 (MQ=255)                                                      gCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGtcat                                                                          <  2:272907/140‑1 (MQ=255)                                                          tttGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGt                                                                      <  1:827145/140‑1 (MQ=255)                                                            tGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGtcat                                                                          <  1:1362492/134‑1 (MQ=255)                                                            tGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGtcat                                                                          >  2:1362492/1‑134 (MQ=255)                                                            tGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCt                                                                    >  1:1086442/1‑140 (MQ=255)                                                            tGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCt                                                                    >  1:243505/1‑140 (MQ=255)                                                                ggCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGc                                                                <  2:613955/140‑1 (MQ=255)                                                                     gATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGcc                                                           <  2:402962/140‑1 (MQ=255)                                                                      aTAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCa                                                          >  1:711076/1‑140 (MQ=255)                                                                        aaGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACA‑TTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTg                                                       >  2:1030895/1‑140 (MQ=255)                                                                                tCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGct                                                >  1:312374/1‑140 (MQ=255)                                                                                      tCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGgtg                                          <  2:711076/140‑1 (MQ=255)                                                                                              ggCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTa                                  <  2:211826/140‑1 (MQ=255)                                                                                              ggCATAAAACAAGCGCCCTTTGGTGGGTTTTTCCCCCCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTa                                  <  2:1204445/140‑1 (MQ=37)                                                                                                cATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACGTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACg                                <  1:472151/140‑1 (MQ=255)                                                                                                cATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACg                                <  2:1127958/140‑1 (MQ=255)                                                                                                    aaCAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTAc                            <  1:244134/140‑1 (MQ=255)                                                                                                    aaCAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTAc                            <  2:312374/140‑1 (MQ=255)                                                                                                      cAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCa                          <  1:1077072/140‑1 (MQ=255)                                                                                                      cAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCa                          <  2:90333/140‑1 (MQ=255)                                                                                                      cAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCa                          <  1:1289968/140‑1 (MQ=255)                                                                                                      cAAAGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCa                          <  1:640094/140‑1 (MQ=255)                                                                                                         aGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGc                       >  1:142836/1‑140 (MQ=255)                                                                                                         aGCGCACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGc                       >  2:1278043/1‑140 (MQ=255)                                                                                                          gcgcACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTa                                  <  1:1154592/128‑1 (MQ=255)                                                                                                          gcgcACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTa                                  >  2:1154592/1‑128 (MQ=255)                                                                                                           cgcACTTTGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCta                     <  2:127841/140‑1 (MQ=255)                                                                                                                tttGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCa                          <  1:743855/130‑1 (MQ=255)                                                                                                                tttGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCa                          >  2:743855/1‑130 (MQ=255)                                                                                                                  tGCTGGTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCa              <  1:1273257/140‑1 (MQ=255)                                                                                                                       gTCTGTTCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGcaca         <  1:875362/140‑1 (MQ=255)                                                                                                                            ttCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaataat    >  1:484914/1‑140 (MQ=255)                                                                                                                            ttCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaataat    >  1:626793/1‑140 (MQ=255)                                                                                                                            ttCCCCTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaataat    >  2:79910/1‑140 (MQ=255)                                                                                                                              ccccTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCa  >  1:494095/1‑140 (MQ=255)                                                                                                                              ccccTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCa  >  2:680476/1‑140 (MQ=255)                                                                                                                                 cTCGCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGca           <  1:1111229/128‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      ACATTGCTAATGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCA  >  NC_000913/4549723‑4549987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑