Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	385,574	A→G	S115G (AGC→GGC)	tauA →	taurine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	385,574	0	A	G	100.0%	204.7 / NA	59	S115G (AGC→GGC)	tauA	taurine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/31);  total (28/31)

TTCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCAGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCG  >  NC_000913/385438‑385709                                                                                                                                         |                                                                                                                                        ttCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCa                                                                                                                                      <  1:1395250/140‑1 (MQ=255)  tCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCaa                                                                                                                                     <  2:1440467/140‑1 (MQ=255)   cAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCaaa                                                                                                                                    >  2:1441138/1‑140 (MQ=255)         aCGTGCAAATCGGCAACCTCGGTTCCAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGaa                                                                                                                              >  2:1235569/1‑140 (MQ=255)           gTGCAAATCGGCAACCTCGGTTCCAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGTGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGa                                                                                                                            >  1:655722/1‑140 (MQ=255)           gTGCAAATCGGCAACCTCGGTTCCAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGa                                                                                                                            >  2:842348/1‑140 (MQ=255)                    ggCAACCTCGGTTCCAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTgg                                                                                                                   >  1:19952/1‑140 (MQ=255)                          cTCGGTTCCAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACg                                                                                                             <  2:97121/140‑1 (MQ=255)                          cTCGGTTCCAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACg                                                                                                             <  2:1047682/140‑1 (MQ=255)                             ggTTCCAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCAt                                                                                                          >  1:1266957/1‑140 (MQ=255)                             ggTTCCAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCAt                                                                                                          >  1:571203/1‑140 (MQ=255)                               ttCCAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCg                                                                                                        <  1:1441138/140‑1 (MQ=255)                                  cAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCg                                                                                                     <  2:571203/140‑1 (MQ=255)                                  cAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCg                                                                                                     <  1:1294956/140‑1 (MQ=255)                                    gCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTa                                                                                                   <  2:989059/140‑1 (MQ=255)                                    gCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTa                                                                                                   >  1:349088/1‑140 (MQ=255)                                        gTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGt                                                                                               <  1:517600/140‑1 (MQ=255)                                         ttAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGtt                                                                                              >  2:106123/1‑140 (MQ=255)                                         ttAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGtt                                                                                              >  2:807401/1‑140 (MQ=255)                                                ttGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTcc                                                                                       >  1:395633/1‑140 (MQ=255)                                                 tGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTcca                                                                                      <  2:395633/140‑1 (MQ=255)                                                 tGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTcca                                                                                      <  1:842348/140‑1 (MQ=255)                                                 tGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTcca                                                                                      <  2:349088/140‑1 (MQ=255)                                                    agccagccAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTccacca                                                                                   <  2:482625/140‑1 (MQ=255)                                                     gccagccaACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGTGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCcaccac                                                                                  <  2:655722/140‑1 (MQ=255)                                                     gccagccaACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCcaccac                                                                                  <  1:1235569/140‑1 (MQ=255)                                                     gccagccaACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCcaccac                                                                                  <  1:807401/140‑1 (MQ=255)                                                                ggTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTcca                                                                                      >  2:1356070/1‑125 (MQ=255)                                                                ggTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTcca                                                                                      <  1:1356070/125‑1 (MQ=255)                                                                       aTTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTggc                                                                >  1:1100791/1‑140 (MQ=255)                                                                                 tcttGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGaaa                                                      >  1:474113/1‑140 (MQ=255)                                                                                      cTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTg                                                 >  1:1163372/1‑140 (MQ=255)                                                                                       tGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTgg                                                <  1:750730/140‑1 (MQ=255)                                                                                        ggCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTggg                                               >  1:1386127/1‑140 (MQ=255)                                                                                        ggCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTggg                                               >  1:213884/1‑140 (MQ=255)                                                                                          cGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTgg                                                >  1:19721/1‑137 (MQ=255)                                                                                          cGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTgg                                                >  2:1421225/1‑137 (MQ=255)                                                                                          cGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTgg                                                <  1:1421225/137‑1 (MQ=255)                                                                                          cGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTgg                                                <  2:19721/137‑1 (MQ=255)                                                                                          cGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGc                                             >  1:23856/1‑140 (MQ=255)                                                                                             cAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCAtt                                          <  2:474113/140‑1 (MQ=255)                                                                                                     ggTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCgg                                  >  1:169527/1‑140 (MQ=255)                                                                                                      gTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCggg                                 <  2:213884/140‑1 (MQ=255)                                                                                                      gTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCggg                                 <  2:1100791/140‑1 (MQ=255)                                                                                                      gTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCAGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCggg                                 <  2:1119279/140‑1 (MQ=255)                                                                                                        aaCTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCa                               <  2:490118/140‑1 (MQ=255)                                                                                                          cTCCGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAg                             >  2:1089402/1‑140 (MQ=255)                                                                                                            ccGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTg                           <  1:899556/140‑1 (MQ=255)                                                                                                             cGAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTgg                          >  1:394675/1‑140 (MQ=255)                                                                                                              gAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGga                         <  2:169527/140‑1 (MQ=255)                                                                                                              gAAGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGga                         <  2:294523/140‑1 (MQ=255)                                                                                                               aaGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGgag                        >  1:1351350/1‑140 (MQ=255)                                                                                                               aaGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGgag                        >  1:466430/1‑140 (MQ=255)                                                                                                                aGCGCTGGTGGTAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGgaga                       <  2:1386127/140‑1 (MQ=255)                                                                                                                           tAAAGAAAACTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTg            <  1:106123/140‑1 (MQ=255)                                                                                                                                   aCTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGTCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAgccgcc    >  2:1369630/1‑140 (MQ=255)                                                                                                                                    cTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGccgccc   <  2:394675/140‑1 (MQ=255)                                                                                                                                    cTATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGccgccc   >  2:390845/1‑140 (MQ=255)                                                                                                                                     tATCGGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGccgcccg  >  2:1048115/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TTCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCAAAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCAGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCG  >  NC_000913/385438‑385709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑