Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	457,597	C→T	H58Y (CAT→TAT)	clpX →	ATP‑dependent Clp protease ATP‑binding subunit ClpX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	457,597	0	C	T	100.0%	245.9 / NA	70	H58Y (CAT→TAT)	clpX	ATP‑dependent Clp protease ATP‑binding subunit ClpX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/30);  total (40/30)

TGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCG  >  NC_000913/457468‑457732                                                                                                                                  |                                                                                                                                        tGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACg                                                                                                                               <  2:1432280/140‑1 (MQ=255)  gCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGc                                                                                                                              <  2:219520/140‑1 (MQ=255)   ctctTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCa                                                                                                                             >  1:746230/1‑140 (MQ=255)     ctTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGa                                                                                                                                  <  2:153234/133‑1 (MQ=255)     ctTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGa                                                                                                                                  >  1:153234/1‑133 (MQ=255)              gCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCg                                                                                                                  <  1:144340/140‑1 (MQ=255)                      cAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCa                                                                                                          <  1:449809/140‑1 (MQ=255)                           tGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGaaa                                                                                                     <  2:822282/140‑1 (MQ=255)                                 gcgcAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCa                                                                                               <  1:1360500/140‑1 (MQ=255)                                  cgcAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACTACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCaa                                                                                              >  2:627070/1‑140 (MQ=255)                                  cgcAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCaa                                                                                              >  1:4906/1‑140 (MQ=255)                                  cgcAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCaa                                                                                              >  1:1029940/1‑140 (MQ=255)                                  cgcAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCaa                                                                                              >  2:390362/1‑140 (MQ=255)                                       gCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAaccacc                                                                                         >  2:193853/1‑140 (MQ=255)                                       gCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAaccacc                                                                                         >  2:492370/1‑140 (MQ=255)                                         tGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTg                                                                                       <  1:193853/140‑1 (MQ=255)                                          gATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTgg                                                                                      >  1:945447/1‑140 (MQ=255)                                           aTTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGa                                                                                     <  1:390362/140‑1 (MQ=255)                                           aTTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGa                                                                                     <  2:1137185/140‑1 (MQ=255)                                            ttGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGAc                                                                                    <  2:945447/140‑1 (MQ=255)                                                   tCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACg                                                                             >  1:1079961/1‑140 (MQ=255)                                                   tCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACg                                                                             >  1:1303365/1‑140 (MQ=255)                                                      aTCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTa                                                                          <  1:923362/140‑1 (MQ=255)                                                            gtATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGcc                                                                    <  2:1277267/140‑1 (MQ=255)                                                                  cTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAAc                                                              >  1:657840/1‑140 (MQ=255)                                                                  cTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAAc                                                              >  1:925256/1‑140 (MQ=255)                                                                   tGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACa                                                             <  1:246758/140‑1 (MQ=255)                                                                     cgacgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAgg                                                           >  2:426857/1‑140 (MQ=255)                                                                     cgacgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAgg                                                           >  1:968301/1‑140 (MQ=255)                                                                     cgacgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAgg                                                           >  1:1142534/1‑140 (MQ=255)                                                                       acgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCg                                                         >  1:771695/1‑140 (MQ=255)                                                                       acgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCg                                                         >  1:1434286/1‑140 (MQ=255)                                                                       acgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCg                                                         <  2:925588/140‑1 (MQ=255)                                                                       acgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCg                                                         >  2:309074/1‑140 (MQ=255)                                                                       acgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCg                                                         >  2:1318521/1‑140 (MQ=255)                                                                        cgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGa                                                        >  1:1131955/1‑140 (MQ=255)                                                                          aaTGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGaaa                                                      >  2:701853/1‑140 (MQ=255)                                                                               gtTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGt                                                 >  2:317077/1‑140 (MQ=255)                                                                                  gATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCt                                              <  1:1318521/140‑1 (MQ=255)                                                                                      tATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCg                                          >  2:806609/1‑140 (MQ=255)                                                                                         gTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTc                                       >  2:1254895/1‑140 (MQ=255)                                                                                         gTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTc                                       >  2:824922/1‑140 (MQ=255)                                                                                          tAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcg                                      <  1:317077/140‑1 (MQ=255)                                                                                          tAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcg                                      >  2:76861/1‑140 (MQ=255)                                                                                          tAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcg                                      <  2:657840/140‑1 (MQ=255)                                                                                           aaCGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcgc                                     <  2:4906/140‑1 (MQ=255)                                                                                            aCGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcgcg                                    <  1:492370/140‑1 (MQ=255)                                                                                             cGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTTGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCgg                                   >  2:485984/1‑140 (MQ=255)                                                                                                  tcatTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcg                                      <  2:266037/132‑1 (MQ=255)                                                                                                  tcatTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcg                                      >  1:266037/1‑132 (MQ=255)                                                                                                  tcatTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcg                                      >  1:366098/1‑132 (MQ=255)                                                                                                  tcatTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcg                                      <  2:366098/132‑1 (MQ=255)                                                                                                  tcatTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATAc                              <  2:968301/140‑1 (MQ=255)                                                                                                   catTCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACa                             <  2:925256/140‑1 (MQ=255)                                                                                                    atTCGCGAAGAGATTAAAGAAGTTACTAAGCTTCGAGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACaa                            <  2:1029940/140‑1 (MQ=255)                                                                                                     ttCGCGAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAAc                           <  2:746230/140‑1 (MQ=255)                                                                                                        gcgAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCgg                                         >  1:442938/1‑123 (MQ=255)                                                                                                        gcgAAGAGATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCgg                                         <  2:442938/123‑1 (MQ=255)                                                                                                            agagATTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACa                    <  1:1458373/140‑1 (MQ=255)                                                                                                                aTTAAAGAAGTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACCGGCGAAAAAAGTGCTGGCGGCCCCGGTATACAACCCTTACcaaac                >  2:1253413/1‑138 (MQ=255)                                                                                                                        agTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTTGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCa        <  1:485984/140‑1 (MQ=255)                                                                                                                        agTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCa        >  2:951183/1‑140 (MQ=255)                                                                                                                        agTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCACTACAAACGTCTGCGCa        >  2:799258/1‑140 (MQ=255)                                                                                                                         gTTGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCaa       >  2:632736/1‑140 (MQ=255)                                                                                                                          ttGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAAc      >  2:274660/1‑140 (MQ=255)                                                                                                                          ttGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAAc      >  2:239506/1‑140 (MQ=255)                                                                                                                          ttGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAAc      >  2:882991/1‑140 (MQ=255)                                                                                                                          ttGCACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAAc      >  1:815707/1‑140 (MQ=255)                                                                                                                             cACCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAATGTCTGCGGCACGGc   >  2:903441/1‑140 (MQ=255)                                                                                                                              aCCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCg  <  2:1131955/140‑1 (MQ=255)                                                                                                                              aCCGTATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCg  <  1:1253413/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        TGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCG  >  NC_000913/457468‑457732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑