Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	485,714	A→G	intergenic (‑95/‑47)	acrA ← / → acrR	multidrug efflux pump membrane fusion lipoprotein AcrA/DNA‑binding transcriptional repressor AcrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	485,714	0	A	G	100.0%	183.5 / NA	53	intergenic (‑95/‑47)	acrA/acrR	multidrug efflux pump membrane fusion lipoprotein AcrA/DNA‑binding transcriptional repressor AcrR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/25);  total (28/25)

TCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATACATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGG  >  NC_000913/485577‑485842                                                                                                                                          |                                                                                                                                 tCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGtacatgca                                                                                                                                >  1:1340451/1‑140 (MQ=255)     aaCGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTca                                                                                                                            <  2:801778/140‑1 (MQ=255)          ccGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAat                                                                                                                       <  1:1113895/140‑1 (MQ=255)                gagGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAatgtatgt                                                                                                                 <  1:840894/140‑1 (MQ=255)                        aaCCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTaa                                                                                                         <  1:930034/140‑1 (MQ=255)                             tctGTTTTTGTTCATATGTAAACCTCGAGTTTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCt                                                                                                    >  1:144304/1‑140 (MQ=255)                             tctGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCt                                                                                                    >  1:1053220/1‑140 (MQ=255)                             tctGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCt                                                                                                    >  1:84011/1‑140 (MQ=255)                                       ttCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCAc                                                                                          <  2:1340451/140‑1 (MQ=255)                                                      cGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGa                                                                           >  1:162420/1‑140 (MQ=255)                                                      cGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGa                                                                           >  1:276531/1‑140 (MQ=255)                                                        aGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGaaa                                                                         >  1:867206/1‑140 (MQ=255)                                                            tCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAAc                                                                      <  2:280712/139‑1 (MQ=255)                                                            tCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAAc                                                                      >  1:280712/1‑139 (MQ=255)                                                                   tCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACaag                                                              <  2:130075/140‑1 (MQ=255)                                                                       aTTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAgc                                                          >  2:190044/1‑140 (MQ=255)                                                                           gtcaatggtcaaAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGaaaa                                                                        <  2:341971/122‑1 (MQ=255)                                                                           gtcaatggtcaaAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGaaaa                                                                        >  1:341971/1‑122 (MQ=255)                                                                           gtcaatggtcaaAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCaa                                                      >  1:1300096/1‑140 (MQ=255)                                                                            tcaatggtcaaAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACaa                                                               >  2:558485/1‑130 (MQ=255)                                                                            tcaatggtcaaAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACaa                                                               <  1:558485/130‑1 (MQ=255)                                                                                tggtcaaAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAAc                                                 <  2:144304/140‑1 (MQ=255)                                                                                         gTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAaca                                        <  2:1300096/140‑1 (MQ=255)                                                                                         gTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAaca                                        <  2:930997/140‑1 (MQ=255)                                                                                          ttAATAAACCAATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAacac                                       >  2:977614/1‑140 (MQ=255)                                                                                                    cATTGCTGCGTTTAAATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGAtg                             <  2:551924/140‑1 (MQ=255)                                                                                                    cATTGCTGCGTTTAAATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGAtg                             >  1:551924/1‑140 (MQ=255)                                                                                                     aTTGCTGCGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGAtgt                            <  1:847657/140‑1 (MQ=255)                                                                                                       tgctgcGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTgg                          <  2:84011/140‑1 (MQ=255)                                                                                                       tgctgcGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTgg                          >  2:354649/1‑140 (MQ=255)                                                                                                        gctgcGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGc                         >  2:648961/1‑140 (MQ=255)                                                                                                          tgcGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGctc                       <  1:771097/140‑1 (MQ=255)                                                                                                            cGTTTATATTATCGTCGTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTa                     >  2:1083807/1‑140 (MQ=255)                                                                                                                       tcgtcgTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTtctc          >  1:294908/1‑140 (MQ=255)                                                                                                                       tcgtcgTGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTtctc          >  2:156418/1‑140 (MQ=255)                                                                                                                        cgtcgtGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTca         >  2:1200517/1‑140 (MQ=255)                                                                                                                        cgtcgtGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTca         >  2:655079/1‑140 (MQ=255)                                                                                                                        cgtcgtGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTca         >  2:771296/1‑140 (MQ=255)                                                                                                                         gtcgtGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTa                     >  1:808980/1‑127 (MQ=255)                                                                                                                         gtcgtGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTa                     <  2:808980/127‑1 (MQ=255)                                                                                                                         gtcgtGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTcac        <  2:162420/140‑1 (MQ=255)                                                                                                                            gtGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCAcagc     >  1:776032/1‑140 (MQ=255)                                                                                                                            gtGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCAcagc     <  2:1053220/140‑1 (MQ=255)                                                                                                                            gtGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCAcag      >  2:809665/1‑139 (MQ=255)                                                                                                                            gtGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCAcag      <  1:809665/139‑1 (MQ=255)                                                                                                                             tGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCAcagca    >  1:719695/1‑140 (MQ=255)                                                                                                                             tGCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCAcagca    <  1:354649/140‑1 (MQ=255)                                                                                                                              gCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTcaca       <  1:44861/136‑1 (MQ=255)                                                                                                                              gCTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTcaca       >  2:44861/1‑136 (MQ=255)                                                                                                                               cTATGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAgg  <  1:156418/140‑1 (MQ=255)                                                                                                                                 aTGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTcaca       <  2:1172282/133‑1 (MQ=255)                                                                                                                                 aTGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTcaca       >  1:1172282/1‑133 (MQ=255)                                                                                                                                 aTGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCAcagca    >  1:866063/1‑136 (MQ=255)                                                                                                                                 aTGGTACATGCATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCAcagca    <  2:866063/136‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 TCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAACCCATTGCTGCGTTTATATTATCGTCGTGCTATGGTACATACATTCACAAATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGG  >  NC_000913/485577‑485842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑