Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 40,458 G→A L492F (CTC→TTC)  caiT ← L‑carnitine:gamma‑butyrobetaine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091340,4580GA100.0% 163.2 / NA 47L492F (CTC→TTC) caiTL‑carnitine:gamma‑butyrobetaine antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/23);  total (24/23)

CTGGCCATCAGTTCGCGGATACCGGCGACAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGACCAACCGATACGCACCAG  >  NC_000913/40322‑40593
                                                                                                                                        |                                                                                                                                       
cTGGCCATCAGTTCGCGGATACCGGCGACAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGt                                                                                                                                      <  1:1246110/140‑1 (MQ=255)
cTGGCCATCAGTTCGCGGATACCGGCGACAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGt                                                                                                                                      <  1:749184/140‑1 (MQ=255)
cTGGCCATCAGTTCGCGGATACCGGCGACAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGt                                                                                                                                      <  1:1329990/140‑1 (MQ=255)
  ggCCATCAGTTCGCGGATACCGGCGACAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCa                                                                                                                                    >  1:1431589/1‑140 (MQ=255)
  ggCCATCAGTTCGCGGATACCGGCGACAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCa                                                                                                                                    <  1:293625/140‑1 (MQ=255)
    ccATCAGTTCGCGGATACCGGCGACAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCAcc                                                                                                                                  >  1:666219/1‑140 (MQ=255)
           ttCGCGGATACCGGCGACAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGt                                                                                                                           <  1:832154/140‑1 (MQ=255)
                    aCCGGCGACAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACgaaga                                                                                                                  <  1:1261718/140‑1 (MQ=255)
                     ccGGCGACAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACgaagaa                                                                                                                 >  1:323528/1‑140 (MQ=255)
                     ccGGCGACAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACgaagaa                                                                                                                 >  2:773725/1‑140 (MQ=255)
                            cAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAAc                                                                                                                >  1:735288/1‑134 (MQ=255)
                            cAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAAc                                                                                                                <  2:735288/134‑1 (MQ=255)
                            cAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCggg                                                                                                          >  1:207002/1‑140 (MQ=255)
                              aaCAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAg                                                                                                              >  2:889636/1‑134 (MQ=255)
                              aaCAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAg                                                                                                              <  1:889636/134‑1 (MQ=255)
                                   ttCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCa                                                                                                        <  2:580794/135‑1 (MQ=255)
                                   ttCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCa                                                                                                        >  1:580794/1‑135 (MQ=255)
                                   ttCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCAtcctc                                                                                                   >  1:351365/1‑140 (MQ=255)
                                     ccTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCg                                                                                                 <  2:351365/140‑1 (MQ=255)
                                       tGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGc                                                                                               <  1:694615/140‑1 (MQ=255)
                                        gCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGc                                                                                               <  1:931484/139‑1 (MQ=255)
                                        gCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGc                                                                                               >  2:931484/1‑139 (MQ=255)
                                             tcatTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAAt                                                                                         >  1:424273/1‑140 (MQ=255)
                                                         aaaTCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTgtaa                                                                             >  1:1099582/1‑137 (MQ=255)
                                                                tCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCCGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAAt                                                                             >  1:617594/1‑133 (MQ=255)
                                                                tCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCCGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAAt                                                                             <  2:617594/133‑1 (MQ=255)
                                                                          tctTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTTATCGGGTTTAgggcccccc                                                            >  2:678437/1‑133 (MQ=255)
                                                                           ctTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGTCGCCGAg                                                           <  1:271730/140‑1 (MQ=255)
                                                                           ctTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAg                                                           >  1:1246223/1‑140 (MQ=255)
                                                                             tGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGGTTGAAACGGGTTTTGgccgcccggcg                                                         >  2:732884/1‑135 (MQ=255)
                                                                              gATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAgcgc                                                        <  2:669633/140‑1 (MQ=255)
                                                                                 atTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCcag                                                     <  1:773725/140‑1 (MQ=255)
                                                                                      gggggTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTGATCGGGTTTTGGCCGCCGAGCGCCCGCACaa                                                >  2:135202/1‑140 (MQ=255)
                                                                                      gggggTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGaa                                                <  2:1431589/140‑1 (MQ=255)
                                                                                      gggggTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGaa                                                <  2:207002/140‑1 (MQ=255)
                                                                                             aattaatCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGTTTTAGGCCGCCGAGCGCCCGCAGAACAAAAcc                                         >  1:213323/1‑140 (MQ=255)
                                                                                                    cTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATg                                  <  1:600110/140‑1 (MQ=255)
                                                                                                       tCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGTTTTAAGCCGCCGAGCGCCCGCAGAAAAAAAACGATaaatcca                               >  2:207776/1‑135 (MQ=255)
                                                                                                       tCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGTTTTAAGCCGCCCAGCGCCCGCAGAAAAAAAACCATaaaacca                               >  2:643146/1‑135 (MQ=255)
                                                                                                       tCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGGTTTTATTGGGTTTAGGCCCCCCCGGGCCCGCAGAAAAAAAACGAAAAAGccc                               >  1:703727/1‑139 (MQ=255)
                                                                                                        ccAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTTAGGCCGCCGCGCGCCCGCCGAAAAAAACCCCTAAAGCCaa                              >  2:600110/1‑140 (MQ=255)
                                                                                                        ccAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCaa                              <  2:1162676/140‑1 (MQ=255)
                                                                                                         cAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCAAc                             <  2:164701/140‑1 (MQ=255)
                                                                                                         cAGTTCTGTTTCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCAAc                             <  1:38593/140‑1 (MQ=255)
                                                                                                                 tttCGCGTCTTTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAATCGGGTTTAGGCCGCCCCGCGCCAGCAGAAAAATAACCAAAATGCCAACCAGAAATg                     >  2:38593/1‑140 (MQ=255)
                                                                                                                 tttCGCGTC‑TTAATAAAGGAGAACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGAGATAATGGCGGTTTGTATCGGGTTCAAGCCGCCGCGCGCCCGCAGAAAAAAAACCATAAAGCCAACCaaaaatta                    >  2:1369130/1‑136 (MQ=255)
                                                                                                                                    gagaACGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCAATAATGCCAACCAGAATTGACCAACCGATACGCACcag  <  2:1246223/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CTGGCCATCAGTTCGCGGATACCGGCGACAAACAGTTCCTGCTCATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGACCAACCGATACGCACCAG  >  NC_000913/40322‑40593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑