Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	555,234	A→G	E208E (GAA→GAG)	cysS →	cysteine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	555,234	0	A	G	100.0%	268.8 / NA	78	E208E (GAA→GAG)	cysS	cysteine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/41);  total (37/41)

CTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAAC  >  NC_000913/555097‑555366                                                                                                                                          |                                                                                                                                     cTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTg                                                                                                                                    >  2:1063318/1‑140 (MQ=255)   gcaggcagGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGtt                                                                                                                                  <  1:953063/140‑1 (MQ=255)     aggcaggcGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCg                                                                                                                                <  1:1001568/140‑1 (MQ=255)           gcgcgcgcGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATg                                                                                                                          >  1:311420/1‑140 (MQ=255)              cgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAAc                                                                                                                       >  1:1084248/1‑140 (MQ=255)                cgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTg                                                                                                                     >  1:120819/1‑140 (MQ=255)                  cgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCa                                                                                                                   <  2:33795/140‑1 (MQ=255)                  cgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCa                                                                                                                   <  1:208428/140‑1 (MQ=255)                     tGACGTGGTCGACGACAAACGCAACCTAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAagc                                                                                                                <  1:1031570/140‑1 (MQ=255)                      gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAagca                                                                                                               <  2:975561/140‑1 (MQ=255)                       aCGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAagcag                                                                                                              <  2:781168/140‑1 (MQ=255)                        cGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAagcagc                                                                                                             <  1:993829/140‑1 (MQ=255)                                acgacAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAAc                                                                                                     <  2:1374111/140‑1 (MQ=255)                                  gacAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCa                                                                                                   >  1:63571/1‑140 (MQ=255)                                  gacAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCa                                                                                                   <  2:311420/140‑1 (MQ=255)                                  gacAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCa                                                                                                   <  2:92177/140‑1 (MQ=255)                                       aCGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTg                                                                                              >  2:337228/1‑140 (MQ=255)                                         gCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGat                                                                                            <  2:120819/140‑1 (MQ=255)                                           aaCCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCa                                                                                                   <  2:349960/131‑1 (MQ=255)                                           aaCCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCa                                                                                                   >  1:349960/1‑131 (MQ=255)                                              ccAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCa                                                                                       >  1:183647/1‑140 (MQ=255)                                               cAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCAc                                                                                      <  2:183647/140‑1 (MQ=255)                                               cAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCAc                                                                                      >  1:431493/1‑140 (MQ=255)                                                       ttCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCAcggcggcgg                                                                              >  2:193312/1‑140 (MQ=255)                                                       ttCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCAcggcggcgg                                                                              >  2:125164/1‑140 (MQ=255)                                                         cGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGtt                                                                            >  1:1205234/1‑140 (MQ=255)                                                         cGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGtt                                                                            <  2:1229328/140‑1 (MQ=255)                                                          gTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTc                                                                           >  1:537887/1‑140 (MQ=255)                                                              tgtgGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGAc                                                                       >  1:239676/1‑140 (MQ=255)                                                                tgGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGtt                                                                            <  1:1201313/133‑1 (MQ=255)                                                                tgGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGtt                                                                            >  2:1201313/1‑133 (MQ=255)                                                                tgGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCt                                                                     <  2:1084248/140‑1 (MQ=255)                                                                  gAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGa                                                                   <  2:266082/140‑1 (MQ=255)                                                                  gAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGa                                                                   <  1:125164/140‑1 (MQ=255)                                                                  gAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGa                                                                   <  1:193312/140‑1 (MQ=255)                                                                          cGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGtt                                                                            <  1:908446/123‑1 (MQ=255)                                                                          cGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGtt                                                                            >  2:908446/1‑123 (MQ=255)                                                                          cGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCg                                                           >  2:1304798/1‑140 (MQ=255)                                                                          cGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCg                                                           >  2:1439372/1‑140 (MQ=255)                                                                                 gggCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCacg                                                    >  2:1058919/1‑140 (MQ=255)                                                                                      aaCCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCacgaaaac                                               >  1:866461/1‑140 (MQ=255)                                                                                          gAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCacgaaaacgaaa                                           >  1:924243/1‑140 (MQ=255)                                                                                          gAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCacgaaaacgaaa                                           >  2:1251392/1‑140 (MQ=255)                                                                                          gAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCCGATGTTCCCGCACCacgaaaacgaaa                                           >  1:769587/1‑140 (MQ=255)                                                                                           aGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAAt                                          >  2:252643/1‑140 (MQ=255)                                                                                             cTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATcg                                        <  2:431493/140‑1 (MQ=255)                                                                                               ggCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATcgcg                                      <  2:932972/140‑1 (MQ=255)                                                                                                gCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCgcgc                                     >  1:519575/1‑140 (MQ=255)                                                                                                  cGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAg                                   >  2:1324744/1‑140 (MQ=255)                                                                                                   gTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGt                                  <  1:1251392/140‑1 (MQ=255)                                                                                                     ctcCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCTGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTcc                                >  1:379911/1‑140 (MQ=255)                                                                                                        cGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc                             <  1:172504/140‑1 (MQ=255)                                                                                                         gTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCt                            <  1:348713/140‑1 (MQ=255)                                                                                                               cgcgGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGccc                      >  1:320776/1‑140 (MQ=255)                                                                                                               cgcgGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGccc                      >  1:640713/1‑140 (MQ=255)                                                                                                               cgcgGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGccc                      >  1:202746/1‑140 (MQ=255)                                                                                                               cgcgGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGccc                      >  2:91908/1‑140 (MQ=255)                                                                                                               cgcgGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGccc                      >  2:414737/1‑140 (MQ=255)                                                                                                               cgcgGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGccc                      >  2:485925/1‑140 (MQ=255)                                                                                                                 cgGGTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCat                    <  2:866461/140‑1 (MQ=255)                                                                                                                  gggTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCatg                   >  2:951830/1‑140 (MQ=255)                                                                                                                  gggTCGTCCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCatg                   >  1:457544/1‑140 (MQ=255)                                                                                                                     tcgtcCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCatgatg                <  1:337228/140‑1 (MQ=255)                                                                                                                     tcgtcCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCatgatg                <  2:1296197/140‑1 (MQ=255)                                                                                                                       gtcCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGt              >  2:1190845/1‑140 (MQ=255)                                                                                                                       gtcCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGt              <  1:365486/140‑1 (MQ=255)                                                                                                                       gtcCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGt              <  1:1425434/140‑1 (MQ=255)                                                                                                                       gtcCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCCGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGt              <  2:769587/140‑1 (MQ=255)                                                                                                                        tcCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTc             <  2:537887/140‑1 (MQ=255)                                                                                                                        tcCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTc             <  2:63571/140‑1 (MQ=255)                                                                                                                        tcCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTc             <  2:640713/140‑1 (MQ=255)                                                                                                                        tcCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTc             <  1:1167761/140‑1 (MQ=255)                                                                                                                        tcCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTc             <  1:1190845/140‑1 (MQ=255)                                                                                                                        tcCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTc             <  1:201268/140‑1 (MQ=255)                                                                                                                        tcCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTc             <  1:1439372/140‑1 (MQ=255)                                                                                                                        tcCTGGCTGGCACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTc             <  2:924243/140‑1 (MQ=255)                                                                                                                            ggctggcACATTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTa         <  2:457544/140‑1 (MQ=255)                                                                                                                                   acaTTGAGTGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAac  >  2:1172620/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     CTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCGGGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACCTGTGCCCATGATGGTCAGTATGTGAAC  >  NC_000913/555097‑555366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑