Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	585,523	A→G	S37S (AGT→AGC)	ompT ←	outer membrane protease VII (outer membrane protein 3b)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	585,523	0	A	G	100.0%	298.0 / NA	86	S37S (AGT→AGC)	ompT	outer membrane protease VII (outer membrane protein 3b)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/41);  total (45/41)

TGGGGCATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTA  >  NC_000913/585386‑585658                                                                                                                                          |                                                                                                                                        tGGGGCATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCt                                                                                                                                       >  2:602616/1‑140 (MQ=255) tGGGGCATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCt                                                                                                                                       >  2:645665/1‑140 (MQ=255)   gggCATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTaa                                                                                                                                     >  1:932295/1‑140 (MQ=255)      cATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGt                                                                                                                                  >  1:570201/1‑140 (MQ=255)      cATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGt                                                                                                                                  >  2:1028817/1‑140 (MQ=255)      cATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGt                                                                                                                                  >  1:873095/1‑140 (MQ=255)       aTCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTc                                                                                                                                 <  1:1117343/140‑1 (MQ=255)        tCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTcc                                                                                                                                >  2:118896/1‑140 (MQ=255)        tCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTcc                                                                                                                                >  2:1060767/1‑140 (MQ=255)        tCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTcc                                                                                                                                >  2:368862/1‑140 (MQ=255)            aTCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCAt                                                                                                                            <  1:602616/140‑1 (MQ=255)               ccAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTa                                                                                                                         >  2:176765/1‑140 (MQ=255)                 aattaattGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTAtg                                                                                                                       <  1:1060767/140‑1 (MQ=255)                  attaattGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTAtgt                                                                                                                      >  1:1401307/1‑140 (MQ=255)                     aattGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTAtgttgt                                                                                                                   <  2:686627/140‑1 (MQ=255)                        tGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAg                                                                                                                >  1:217790/1‑140 (MQ=255)                        tGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAg                                                                                                                >  1:504168/1‑140 (MQ=255)                         gCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTAt                                                                                                                        <  2:1087130/131‑1 (MQ=255)                         gCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTAt                                                                                                                        >  1:1087130/1‑131 (MQ=255)                          cACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGa                                                                                                              <  1:550491/140‑1 (MQ=255)                           aCCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAg                                                                                                             >  1:549946/1‑140 (MQ=255)                             cTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTa                                                                                                           <  2:387016/140‑1 (MQ=255)                               ttAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTaaa                                                                                                         >  1:1081674/1‑140 (MQ=255)                               ttAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTaaa                                                                                                         >  2:400451/1‑140 (MQ=255)                               ttAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTaaa                                                                                                         >  2:490394/1‑140 (MQ=255)                               ttAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTaaa                                                                                                         >  1:624129/1‑140 (MQ=255)                                   taatTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCTGCATTTATGTTGTCAGGAGTAAACGAt                                                                                                     <  2:932295/140‑1 (MQ=255)                                    aatTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATa                                                                                                    >  1:514050/1‑140 (MQ=255)                                         cAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGAt                                                                                                     >  1:1240991/1‑134 (MQ=255)                                         cAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGAt                                                                                                     <  2:1240991/134‑1 (MQ=255)                                          aGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGtct                                                                                              >  2:1408748/1‑140 (MQ=255)                                              ttATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGt                                                                                          <  1:400451/140‑1 (MQ=255)                                              ttATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGt                                                                                          <  2:747271/140‑1 (MQ=255)                                                 ttGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCTGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGa                                                                                       <  2:587192/140‑1 (MQ=255)                                                 ttGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGa                                                                                       <  2:1401307/140‑1 (MQ=255)                                                 ttGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGa                                                                                       <  1:704955/140‑1 (MQ=255)                                                 ttGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGa                                                                                       >  1:313208/1‑140 (MQ=255)                                                 ttGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGa                                                                                       <  1:645665/140‑1 (MQ=255)                                                   gAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAg                                                                                     <  1:114495/140‑1 (MQ=255)                                                      tttCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGtct                                                                                              <  1:194823/128‑1 (MQ=255)                                                      tttCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGtct                                                                                              >  2:194823/1‑128 (MQ=255)                                                      tttCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCa                                                                                   <  2:1144449/139‑1 (MQ=255)                                                      tttCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCa                                                                                   >  1:1144449/1‑139 (MQ=255)                                                        tCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCaaaa                                                                                >  2:1466422/1‑140 (MQ=255)                                                         ccAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGtc                                                                                               <  2:80956/124‑1 (MQ=255)                                                         ccAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGtc                                                                                               >  1:80956/1‑124 (MQ=255)                                                           aGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAagag                                                                             >  2:1447141/1‑140 (MQ=255)                                                             tCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCt                                                                           <  2:1081674/140‑1 (MQ=255)                                                             tCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCt                                                                           <  2:624129/140‑1 (MQ=255)                                                               gAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGa                                                                         <  1:1218887/140‑1 (MQ=255)                                                                aGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGAt                                                                        <  2:357829/140‑1 (MQ=255)                                                                aGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGAt                                                                        <  2:956393/140‑1 (MQ=255)                                                                aGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGAt                                                                        <  2:555668/140‑1 (MQ=255)                                                                aGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGAt                                                                        <  2:55847/140‑1 (MQ=255)                                                                aGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGAt                                                                        <  1:987644/140‑1 (MQ=255)                                                                aGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGAt                                                                        <  1:1240223/140‑1 (MQ=255)                                                                aGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGAt                                                                        <  1:1408748/140‑1 (MQ=255)                                                                  ttGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCg                                                                      >  2:186498/1‑140 (MQ=255)                                                                  ttGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCg                                                                      >  2:683760/1‑140 (MQ=255)                                                                   tgactgacTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGc                                                                     <  1:1447141/140‑1 (MQ=255)                                                                           ttttCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAgggg                                                             <  1:683760/140‑1 (MQ=255)                                                                                   ctcctTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAAt                                                                  <  2:1375946/127‑1 (MQ=255)                                                                                   ctcctTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAAt                                                                  >  1:1375946/1‑127 (MQ=255)                                                                                   ctcctTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAgg                                                     >  1:819641/1‑140 (MQ=255)                                                                                    tcctTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGa                                                    <  2:485035/140‑1 (MQ=255)                                                                                               cTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGCCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGa                                         >  1:1346753/1‑140 (MQ=255)                                                                                                       acacGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGc                                 <  2:819641/140‑1 (MQ=255)                                                                                                       acacGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGc                                 <  1:1392423/140‑1 (MQ=255)                                                                                                       acacGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGCCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGc                                 <  2:1346753/140‑1 (MQ=255)                                                                                                         acGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGccc                               >  2:155259/1‑140 (MQ=255)                                                                                                            ctctTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCa                            >  1:1250017/1‑140 (MQ=255)                                                                                                            ctctTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCa                            >  1:90388/1‑140 (MQ=255)                                                                                                              ctTTTGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATa                          >  1:840597/1‑140 (MQ=255)                                                                                                               ttttGTTTTTCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATaa                         >  2:869081/1‑140 (MQ=255)                                                                                                                      tttCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTtct                  <  1:155259/140‑1 (MQ=255)                                                                                                                      tttCCGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTtct                  <  2:570201/140‑1 (MQ=255)                                                                                                                         ccGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGtt                    <  2:490728/135‑1 (MQ=255)                                                                                                                         ccGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGtt                    >  1:490728/1‑135 (MQ=255)                                                                                                                         ccGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCa               >  2:226065/1‑140 (MQ=255)                                                                                                                         ccGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCa               <  1:869081/140‑1 (MQ=255)                                                                                                                         ccGCTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCa               >  1:365425/1‑140 (MQ=255)                                                                                                                            cTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTtct                  <  1:728404/134‑1 (MQ=255)                                                                                                                            cTCAGAGTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTtct                  >  2:728404/1‑134 (MQ=255)                                                                                                                                gagTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATc        >  1:588505/1‑140 (MQ=255)                                                                                                                                 agTTCCAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCg       >  1:703260/1‑140 (MQ=255)                                                                                                                                      cAAGGCTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTa  >  2:77847/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TGGGGCATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTA  >  NC_000913/585386‑585658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑