Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	695,009	T→C	intergenic (‑54/‑92)	miaB ← / → ubiF	isopentenyl‑adenosine A37 tRNA methylthiolase/2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	695,009	0	T	C	100.0%	120.7 / NA	36	intergenic (‑54/‑92)	miaB/ubiF	isopentenyl‑adenosine A37 tRNA methylthiolase/2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/19);  total (17/19)

CATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGG  >  NC_000913/694874‑695135                                                                                                                                        |                                                                                                                               cATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTtgc                                                                                                                            >  2:1454345/1‑140 (MQ=255)   tCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTtgctg                                                                                                                          <  1:1454345/140‑1 (MQ=255)         agATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTtg                                                                                                                    <  2:1228815/140‑1 (MQ=255)           aTCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTtgtg                                                                                                                  >  1:265340/1‑140 (MQ=255)           aTCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTtgtg                                                                                                                  >  2:724413/1‑140 (MQ=255)             cGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGAc                                                                                                                <  1:421265/140‑1 (MQ=255)             cGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGAc                                                                                                                <  1:51328/140‑1 (MQ=255)             cGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGAc                                                                                                                <  1:724413/140‑1 (MQ=255)                    cTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATg                                                                                                         <  2:1234201/140‑1 (MQ=255)                          tGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGtt                                                                                                   >  2:868114/1‑140 (MQ=255)                                     cGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTa                                                                                        <  1:868114/140‑1 (MQ=255)                                        tCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAggg                                                                                     >  2:1275118/1‑140 (MQ=255)                                           tCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTa                                                                                        >  1:370649/1‑134 (MQ=255)                                           tCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTa                                                                                        <  2:370649/134‑1 (MQ=255)                                           tCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTa                                                                                  >  1:1401956/1‑140 (MQ=255)                                           tCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTa                                                                                  >  1:484625/1‑140 (MQ=255)                                                cAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAAt                                                                             >  2:871715/1‑140 (MQ=255)                                                cAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAAt                                                                             <  2:299169/140‑1 (MQ=255)                                                     ccAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGt                                                                        <  1:1275118/140‑1 (MQ=255)                                                              aaTATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTaaa                                                               <  2:265340/140‑1 (MQ=255)                                                                        tttttGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCt                                                     <  2:1401956/140‑1 (MQ=255)                                                                          tttGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTaa                                                   <  1:1153810/140‑1 (MQ=255)                                                                             ggTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAg                                                <  1:1121047/140‑1 (MQ=255)                                                                                         gCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCa                                    >  1:197585/1‑140 (MQ=255)                                                                                                       gtagCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATcaacca                      >  1:54492/1‑140 (MQ=255)                                                                                                          gCCAGGAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACg                   <  2:484625/140‑1 (MQ=255)                                                                                                               gAATGCAGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAAt              >  1:123319/1‑140 (MQ=255)                                                                                                                     aGGGCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCAt        >  2:1387910/1‑140 (MQ=255)                                                                                                                       ggCGCATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTg      >  1:1091784/1‑140 (MQ=255)                                                                                                                        gcgcATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGt     <  1:836986/140‑1 (MQ=255)                                                                                                                        gcgcATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGt     <  2:552734/140‑1 (MQ=255)                                                                                                                         cgcATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGa                 >  1:835707/1‑127 (MQ=255)                                                                                                                         cgcATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGa                 <  2:835707/127‑1 (MQ=255)                                                                                                                         cgcATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTc    >  1:535172/1‑140 (MQ=255)                                                                                                                           cATAGTGTAATGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTcgg  >  2:1438259/1‑140 (MQ=255)                                                                                                                                     tGCCTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGt     <  2:894768/127‑1 (MQ=255)                                                                                                                                        |                                                                                                                               CATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGG  >  NC_000913/694874‑695135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑