Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	786,366	A→C	H245P (CAC→CCC)	aroG →	3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	786,366	0	A	C	100.0%	259.4 / NA	75	H245P (CAC→CCC)	aroG	3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (37/38);  total (37/38)

TAATGCCGCCGGTGCGCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCC  >  NC_000913/786232‑786502                                                                                                                                       |                                                                                                                                         tAATGCCGCCGGTGCGCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTg                                                                                                                                     <  1:1425258/140‑1 (MQ=255)   aTGCCGCCGGTGCGCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCt                                                                                                                                   <  2:181018/140‑1 (MQ=255)   aTGCCGCCGGTGCGCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCt                                                                                                                                   <  2:1039587/140‑1 (MQ=255)   aTGCCGCCGGTGCGCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCt                                                                                                                                   <  1:913690/140‑1 (MQ=255)       cgccgGTGCGCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAg                                                                                                                               >  1:126092/1‑140 (MQ=255)        gccgGTGCGCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGt                                                                                                                              <  2:1274057/140‑1 (MQ=255)         ccgGTGCGCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGGGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTg                                                                                                                             >  2:908177/1‑140 (MQ=255)           ggTGCGCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGaa                                                                                                                           >  1:1053662/1‑140 (MQ=255)             tGCGCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAaag                                                                                                                         <  1:1400753/140‑1 (MQ=255)                gccgcACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAaagaag                                                                                                                      >  1:1351163/1‑140 (MQ=255)                  cgcACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAggg                                                                                                                    <  2:1114765/140‑1 (MQ=255)                  cgcACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAggg                                                                                                                    <  1:39240/140‑1 (MQ=255)                     aCTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTg                                                                                                                 >  2:506067/1‑140 (MQ=255)                       tGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGaa                                                                                                               <  2:166862/140‑1 (MQ=255)                         cTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACa                                                                                                             >  1:1354642/1‑140 (MQ=255)                         cTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACa                                                                                                             >  1:1029713/1‑140 (MQ=255)                           tCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACaaa                                                                                                           <  2:1029713/140‑1 (MQ=255)                           tCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACaaa                                                                                                           <  2:774914/140‑1 (MQ=255)                               gTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAg                                                                                                       >  1:802839/1‑140 (MQ=255)                                tCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGGGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAgg                                                                                                      <  2:1053662/140‑1 (MQ=255)                                tCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAgg                                                                                                      <  1:70501/140‑1 (MQ=255)                                   gTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCt                                                                                                   <  1:908177/140‑1 (MQ=255)                                   gTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCt                                                                                                   <  2:1351163/140‑1 (MQ=255)                                   gTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCt                                                                                                   >  2:631717/1‑140 (MQ=255)                                   gTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCt                                                                                                   >  1:1296270/1‑140 (MQ=255)                                     aaCGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGc                                                                                                 >  1:398302/1‑140 (MQ=255)                                     aaCGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGc                                                                                                 <  2:1296270/140‑1 (MQ=255)                                     aaCGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGc                                                                                                 <  2:2075/140‑1 (MQ=255)                                          aaTGGGGGCATTCGGCGATTGTGAATCCCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGca                                                                                            <  1:1030603/140‑1 (MQ=255)                                          aaTGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGca                                                                                            <  2:398302/140‑1 (MQ=255)                                          aaTGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGca                                                                                            <  2:1137878/140‑1 (MQ=255)                                          aaTGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGca                                                                                            <  1:631717/140‑1 (MQ=255)                                          aaTGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGca                                                                                            <  2:802839/140‑1 (MQ=255)                                          aaTGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGca                                                                                            <  2:182359/140‑1 (MQ=255)                                          aaTGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGca                                                                                            <  2:475233/140‑1 (MQ=255)                                           aTGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGcac                                                                                           <  2:120212/140‑1 (MQ=255)                                            tGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGcaca                                                                                          >  2:1101492/1‑140 (MQ=255)                                                              gtgAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCa                                                                         <  2:121136/139‑1 (MQ=255)                                                              gtgAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCa                                                                         >  1:121136/1‑139 (MQ=255)                                                              gtgAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAg                                                                        <  1:506067/140‑1 (MQ=255)                                                              gtgAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAg                                                                        <  1:878869/140‑1 (MQ=255)                                                               tgAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGc                                                                       <  1:1101492/140‑1 (MQ=255)                                                                  aTACCCGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGTAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCAt                                                                    <  2:1301273/140‑1 (MQ=255)                                                                      cAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTa                                                                >  1:1154795/1‑140 (MQ=255)                                                                         cGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACt                                                             >  1:1015605/1‑140 (MQ=255)                                                                         cGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACt                                                             >  1:1430410/1‑140 (MQ=255)                                                                              aCGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTcc                                                        >  1:1420551/1‑140 (MQ=255)                                                                                                  cTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAgat                                    <  2:1303420/140‑1 (MQ=255)                                                                                                    gcgcGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAgatgg                                  >  1:956673/1‑140 (MQ=255)                                                                                                    gcgcGGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAgatgg                                  >  2:1426880/1‑140 (MQ=255)                                                                                                     cgcgGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCaaaaa                                          >  2:1083194/1‑131 (MQ=255)                                                                                                     cgcgGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCaaaaa                                          <  1:1083194/131‑1 (MQ=255)                                                                                                     cgcgGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCaaa                                            <  1:1026613/129‑1 (MQ=255)                                                                                                     cgcgGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCaaa                                            >  2:1026613/1‑129 (MQ=255)                                                                                                     cgcgGCGGTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAgatgga                                 >  1:342902/1‑140 (MQ=255)                                                                                                       cggcggTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAgatggatg                               >  2:848711/1‑140 (MQ=255)                                                                                                          cggTAAAGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGttt                            >  1:924529/1‑140 (MQ=255)                                                                                                              aaaGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGc                        <  2:1354642/140‑1 (MQ=255)                                                                                                              aaaGAGCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGc                        <  2:1420551/140‑1 (MQ=255)                                                                                                                 gagCCTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGa                     <  2:1154795/140‑1 (MQ=255)                                                                                                                    ccTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAgatg                                   >  1:538405/1‑123 (MQ=255)                                                                                                                    ccTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAgatg                                   <  2:538405/123‑1 (MQ=255)                                                                                                                    ccTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGt                  >  1:715754/1‑140 (MQ=255)                                                                                                                    ccTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGt                  >  1:1436235/1‑140 (MQ=255)                                                                                                                    ccTAACTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGt                  >  2:1287180/1‑140 (MQ=255)                                                                                                                       aaCTACAGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTg               >  2:1340238/1‑140 (MQ=255)                                                                                                                             aGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCcagca         >  2:691887/1‑140 (MQ=255)                                                                                                                             aGCGCGAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCcagca         >  1:365320/1‑140 (MQ=255)                                                                                                                               cgcgAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGa       >  1:1016826/1‑140 (MQ=255)                                                                                                                                 cgAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGAtt     >  1:1463550/1‑140 (MQ=255)                                                                                                                                 cgAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGAtt     >  2:926068/1‑140 (MQ=255)                                                                                                                                  gAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTg    <  2:1436235/140‑1 (MQ=255)                                                                                                                                  gAAGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTg    >  1:729726/1‑140 (MQ=255)                                                                                                                                   aaGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGc   >  2:370247/1‑140 (MQ=255)                                                                                                                                    aGCCCGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGcc  >  2:1204560/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         TAATGCCGCCGGTGCGCCGCACTGCTTCCTGTCCGTAACGAAATGGGGGCATTCGGCGATTGTGAATACCAGCGGTAACGGCGATTGCCATATCATTCTGCGCGGCGGTAAAGAGCCTAACTACAGCGCGAAGCACGTTGCTGAAGTGAAAGAAGGGCTGAACAAAGCAGGCCTGCCAGCACAGGTGATGATCGATTTCAGCCATGCTAACTCGTCCAAACAATTCAAAAAGCAGATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCC  >  NC_000913/786232‑786502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑