Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	838,757	A→G	L151L (TTG→CTG)	ybiX ←	PKHD‑type hydroxylase YbiX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	838,757	0	A	G	100.0%	298.5 / NA	86	L151L (TTG→CTG)	ybiX	PKHD‑type hydroxylase YbiX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/40);  total (46/40)

TTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTT  >  NC_000913/838630‑838891                                                                                                                                |                                                                                                                                       ttgtCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGcc                                                                                                                            >  1:1222952/1‑140 (MQ=255) ttgtCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGcc                                                                                                                            >  1:1412176/1‑140 (MQ=255)    tCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGc                                                                                                                         >  1:372404/1‑140 (MQ=255)    tCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGc                                                                                                                         >  1:1082542/1‑140 (MQ=255)    tCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGc                                                                                                                         >  2:622526/1‑140 (MQ=255)    tCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGc                                                                                                                         >  1:1288956/1‑140 (MQ=255)    tCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGc                                                                                                                         >  1:1381335/1‑140 (MQ=255)      cAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCg                                                                                                                       >  1:705256/1‑140 (MQ=255)           cAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCgg                                                                                                                      >  2:419113/1‑136 (MQ=255)           cAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCgg                                                                                                                      <  1:419113/136‑1 (MQ=255)            aaaCAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGtt                                                                                                                 <  1:196747/140‑1 (MQ=255)            aaaCAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGtt                                                                                                                 <  2:161887/140‑1 (MQ=255)              aCAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGtttt                                                                                                               <  1:412562/140‑1 (MQ=255)                 gCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTAcc                                                                                                            <  2:1082542/140‑1 (MQ=255)                  cATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTAccc                                                                                                           >  2:1447957/1‑140 (MQ=255)                  cATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTAcc                                                                                                            >  2:825367/1‑139 (MQ=255)                  cATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTAcc                                                                                                            <  1:825367/139‑1 (MQ=255)                   aTGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTAcc                                                                                                            <  2:684743/138‑1 (MQ=255)                   aTGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTAcc                                                                                                            >  1:684743/1‑138 (MQ=255)                   aTGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTAcc                                                                                                            <  1:147448/138‑1 (MQ=255)                   aTGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTAcc                                                                                                            >  2:147448/1‑138 (MQ=255)                   aTGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCg                                                                                                          >  2:1301905/1‑140 (MQ=255)                      gcgcgcTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCTGGAGTTTTACCCGAtg                                                                                                       <  1:871923/140‑1 (MQ=255)                             ttttATCATCGCGGATCATCGATTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCg                                                                                                <  1:234159/140‑1 (MQ=255)                               ttATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGaa                                                                                              <  2:832832/140‑1 (MQ=255)                               ttATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGaa                                                                                              <  1:1301905/140‑1 (MQ=255)                                 atcatcGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAgg                                                                                            <  2:1319732/140‑1 (MQ=255)                                      cgcgGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCa                                                                                       >  1:243635/1‑140 (MQ=255)                                      cgcgGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCa                                                                                       >  2:624372/1‑140 (MQ=255)                                          gATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTaa                                                                                   >  2:1057996/1‑140 (MQ=255)                                              atcGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGAc                                                                               <  2:374969/140‑1 (MQ=255)                                                cGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCa                                                                             >  2:1116426/1‑140 (MQ=255)                                                     ggATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTcg                                                                        >  2:328266/1‑140 (MQ=255)                                                      gATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTcgc                                                                       <  1:624372/140‑1 (MQ=255)                                                       aTCCACATAAATGATGCCACTCGTACGCCGCGGGTTACTGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTcgcc                                                                      >  1:868973/1‑140 (MQ=255)                                                       aTCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTcgcc                                                                      >  2:782320/1‑140 (MQ=255)                                                       aTCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTcgcc                                                                      <  2:705256/140‑1 (MQ=255)                                                        tCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTcgccg                                                                     <  1:1057996/140‑1 (MQ=255)                                                              tAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCcgtcg                                                               <  2:1288956/140‑1 (MQ=255)                                                                aaTGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTa                                                             <  2:71569/140‑1 (MQ=255)                                                                aaTGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTa                                                             <  2:1412176/140‑1 (MQ=255)                                                                 atgatgCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAg                                                            >  1:789980/1‑140 (MQ=255)                                                                  tgatgCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTcgccg                                                                     <  1:727767/130‑1 (MQ=255)                                                                  tgatgCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTcgccg                                                                     >  2:727767/1‑130 (MQ=255)                                                                    atgCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCtt                                                         >  2:579729/1‑140 (MQ=255)                                                                     tgCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTa                                                             >  2:1239083/1‑135 (MQ=255)                                                                     tgCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTa                                                             <  1:1239083/135‑1 (MQ=255)                                                                      gCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTtg                                                       >  2:1394699/1‑140 (MQ=255)                                                                      gCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTtg                                                       >  1:1317079/1‑140 (MQ=255)                                                                      gCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTtg                                                       >  1:1396790/1‑140 (MQ=255)                                                                      gCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTtg                                                       >  2:652131/1‑140 (MQ=255)                                                                      gCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTtg                                                       >  1:707652/1‑140 (MQ=255)                                                                       ccACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTtgt                                                      >  1:376065/1‑140 (MQ=255)                                                                       ccACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTtgt                                                      >  2:474517/1‑140 (MQ=255)                                                                         aCTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTgg                                                    >  1:397087/1‑140 (MQ=255)                                                                         aCTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTgg                                                    >  2:865094/1‑140 (MQ=255)                                                                          cTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGa                                                   >  1:840542/1‑140 (MQ=255)                                                                           tCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGAt                                                  <  1:474517/140‑1 (MQ=255)                                                                           tCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGAt                                                  <  2:1396790/140‑1 (MQ=255)                                                                           tCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGAt                                                  <  1:865094/140‑1 (MQ=255)                                                                             gTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCg                                                <  2:372404/140‑1 (MQ=255)                                                                              tACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTa                                                             >  2:188619/1‑126 (MQ=255)                                                                              tACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTa                                                             <  1:188619/126‑1 (MQ=255)                                                                                   cgcgGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTaaa                                          >  2:813283/1‑140 (MQ=255)                                                                                     cgGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTaaaaa                                        >  1:264427/1‑140 (MQ=255)                                                                                     cgGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTaaaaa                                        >  1:121533/1‑140 (MQ=255)                                                                                     cgGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTaaaaa                                        <  2:376065/140‑1 (MQ=255)                                                                                     cgGGTTAAGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTaaaaa                                        >  1:735022/1‑140 (MQ=255)                                                                                       ggTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACa                                      <  2:1254689/140‑1 (MQ=255)                                                                                        gTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAg                                     <  2:243635/140‑1 (MQ=255)                                                                                              ggTGTCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGc                               <  2:868973/140‑1 (MQ=255)                                                                                                 gtCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGa                            <  2:735022/140‑1 (MQ=255)                                                                                                 gtCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGCAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGa                            <  2:1317079/140‑1 (MQ=255)                                                                                                  tCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGaa                           >  1:1071017/1‑140 (MQ=255)                                                                                                  tCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGaa                           >  2:684352/1‑140 (MQ=255)                                                                                                  tCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGaa                           >  1:1256129/1‑140 (MQ=255)                                                                                                  tCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGa                            >  2:223673/1‑139 (MQ=255)                                                                                                  tCACGCAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTGTGTGGATCGCTTAAAAACAGCGTGGCAGa                            <  1:223673/139‑1 (MQ=255)                                                                                                       cAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTc                      <  2:397087/140‑1 (MQ=255)                                                                                                       cAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTc                      <  2:121533/140‑1 (MQ=255)                                                                                                       cAATGCAGGCTGCTGGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTc                      <  1:823654/140‑1 (MQ=255)                                                                                                                 tgctgGAGGGATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATc            <  1:813283/140‑1 (MQ=255)                                                                                                                         ggATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGtt    <  2:1256129/140‑1 (MQ=255)                                                                                                                         ggATACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGtt    <  1:684352/140‑1 (MQ=255)                                                                                                                           aTACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGtttt  >  2:8938/1‑140 (MQ=255)                                                                                                                           aTACAGCACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGtttt  >  2:956699/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                       TTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTT  >  NC_000913/838630‑838891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑