Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	906,746	A→G	V3A (GTA→GCA)	ybjS ←	putative NAD(P)H‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	906,746	0	A	G	100.0%	207.0 / NA	60	V3A (GTA→GCA)	ybjS	putative NAD(P)H‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/31);  total (29/31)

ACAAACTCTGCGCCCATTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTACCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGAATAACGCAGGTTTTGTCGATTAATCTGTGCTTT  >  NC_000913/906611‑906865                                                                                                                                        |                                                                                                                        aCAAACTCTGCGCCCATTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTATCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCtt                                                                                                                     <  2:857794/140‑1 (MQ=255)     aCTCTGCGCCCATTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCata                                                                                                                 <  1:1317005/140‑1 (MQ=255)     aCTCTGCGCCCATTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCata                                                                                                                 >  2:1418121/1‑140 (MQ=255)      ctctGCGCCCATTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCatat                                                                                                                <  1:1352018/140‑1 (MQ=255)      ctctGCGCCCATTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCatat                                                                                                                <  2:369923/140‑1 (MQ=255)      ctctGCGCCCATTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCatat                                                                                                                <  2:581738/140‑1 (MQ=255)          gcgcCCATTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCatat                                                                                                                >  1:1106130/1‑136 (MQ=255)          gcgcCCATTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCatat                                                                                                                >  1:1012530/1‑136 (MQ=255)          gcgcCCATTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCatat                                                                                                                <  2:1106130/136‑1 (MQ=255)          gcgcCCATTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCatat                                                                                                                <  2:1012530/136‑1 (MQ=255)          gcgcCCATTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAAt                                                                                                            <  2:693348/140‑1 (MQ=255)            gcCCATTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATcc                                                                                                          >  2:1335327/1‑140 (MQ=255)                aTTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACtg                                                                                                      <  1:1335327/140‑1 (MQ=255)                      tCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGttgttg                                                                                                <  2:1403041/140‑1 (MQ=255)                        cAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTgag                                                                                              <  2:1384623/140‑1 (MQ=255)                         aGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTgaga                                                                                             <  2:946896/140‑1 (MQ=255)                                tGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAAc                                                                                      <  2:763039/140‑1 (MQ=255)                                                 cGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCa                                                                               >  1:311787/1‑130 (MQ=255)                                                 cGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCa                                                                               <  2:311787/130‑1 (MQ=255)                                                 cGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTg                                                                     >  1:547223/1‑140 (MQ=255)                                                   aCCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGt                                                                                    <  2:31516/123‑1 (MQ=255)                                                   aCCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGt                                                                                    >  1:31516/1‑123 (MQ=255)                                                   aCCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGAt                                                                   >  2:449027/1‑140 (MQ=255)                                                   aCCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCAATGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGAt                                                                   >  2:625124/1‑140 (MQ=255)                                                       gTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCAtt                                                                             <  2:218519/126‑1 (MQ=255)                                                       gTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCAtt                                                                             >  1:218519/1‑126 (MQ=255)                                                       gTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTcc                                                               >  1:259131/1‑140 (MQ=255)                                                        tcgctcgcACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTccc                                                              >  2:1065911/1‑140 (MQ=255)                                                        tcgctcgcACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTccc                                                              >  2:1387417/1‑140 (MQ=255)                                                        tcgctcgcACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCAATGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTccc                                                              <  1:625124/140‑1 (MQ=255)                                                           ctcgcACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCa                                                           <  2:547223/140‑1 (MQ=255)                                                              gcACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCatga                                                        <  1:1418121/140‑1 (MQ=255)                                                                   cTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATAGCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCaa                                                   <  1:154885/140‑1 (MQ=255)                                                                      aTGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTccc                                                              >  2:1137925/1‑126 (MQ=255)                                                                      aTGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTccc                                                              <  1:1137925/126‑1 (MQ=255)                                                                      aTGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATgg                                                >  2:799531/1‑140 (MQ=255)                                                                      aTGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATgg                                                >  1:146425/1‑140 (MQ=255)                                                                      aTGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATgg                                                >  2:669308/1‑140 (MQ=255)                                                                      aTGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATg                                                 >  2:642152/1‑139 (MQ=255)                                                                      aTGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATg                                                 <  1:642152/139‑1 (MQ=255)                                                                        gCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGa                                              <  2:259131/140‑1 (MQ=255)                                                                          cTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGaaa                                            <  1:799531/140‑1 (MQ=255)                                                                              cTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATg                                        >  2:1063854/1‑140 (MQ=255)                                                                              cTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATg                                        >  2:1379442/1‑140 (MQ=255)                                                                                ggCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGaa                                      >  2:1345065/1‑140 (MQ=255)                                                                                   aTAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGa                                   >  1:166325/1‑140 (MQ=255)                                                                                    tAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGaa                                  <  1:6677/140‑1 (MQ=255)                                                                                          ctctACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGAATAACGc                            <  1:669308/140‑1 (MQ=255)                                                                                                cgcgTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGAATAACGCAGGttt                      >  2:544582/1‑140 (MQ=255)                                                                                                cgcgTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGAATAACGCAGGttt                      >  2:370430/1‑140 (MQ=255)                                                                                                 gcgTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGAATAACGCAGGtttt                     <  1:1065911/140‑1 (MQ=255)                                                                                                    tttCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGAATAACGCAGGTTTTGTc                  >  2:1132978/1‑140 (MQ=255)                                                                                                         aCCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGTAAGAATAACGCAGGTTTTGTAGAtt              >  2:1303204/1‑139 (MQ=255)                                                                                                         aCCTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGAATAACGCAGGTTTTGTCGAtt              <  1:1303204/139‑1 (MQ=255)                                                                                                           cTAAGCCGCTGGTGGCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGAATAACGCAGGTTTTGTCGATTAAt           <  2:8166/140‑1 (MQ=255)                                                                                                                    tggtggCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGAATAACGCAGGTTTTGTCGATTAATCTGTGCttt  <  1:544582/140‑1 (MQ=255)                                                                                                                    tggtggCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGAATAACGCAGGTTTTGTCGATTAATCTGTGCttt  >  2:501426/1‑140 (MQ=255)                                                                                                                    tggtggCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGAATAACGCAGGTTTTGTCGATTAATCTGTGCttt  >  1:87708/1‑140 (MQ=255)                                                                                                                        ggCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGAATAACGCAGGTTTTGTCGATTAATCt         >  1:7149/1‑129 (MQ=255)                                                                                                                        ggCGCCGGTAACCAGTGCCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGAATAACGCAGGTTTTGTCGATTAATCt         <  2:7149/129‑1 (MQ=255)                                                                                                                                        |                                                                                                                        ACAAACTCTGCGCCCATTTTTTCCAGCAATTTGCCCATTGCCTCGTTGCGACCGGTCGCTCGCACGCTGATGCCTTTCTGGCATAAAAACTCTACCGCGTTTCGACCTAAGCCGCTGGTGGCGCCGGTAACCAGTACCTTCATATCAATCCACTGTTGTTGAGAAAATAACGTGCGCATTCTTCCGTGATTTCCCCCATGATGCAATGGGAAACATGAAAGAATAACGCAGGTTTTGTCGATTAATCTGTGCTTT  >  NC_000913/906611‑906865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑