Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	913,668	C→T	A49A (GCG→GCA)	hcp ←	protein S‑nitrosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	913,668	0	C	T	100.0%	340.9 / NA	95	A49A (GCG→GCA)	hcp	protein S‑nitrosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (52/43);  total (52/43)

AGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGTTTGTTCACATTG  >  NC_000913/913534‑913802                                                                                                                                       |                                                                                                                                       aGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCa                                                                                                                                   <  1:1127416/140‑1 (MQ=255)           cTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGc                                                                                                                         >  1:603398/1‑140 (MQ=255)               aCGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTAGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTTCTGCCCAGGCAGAAAGCCCtt                                                                                                                     >  2:1188809/1‑140 (MQ=255)                  aGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCa                                                                                                                  <  2:912841/140‑1 (MQ=255)                      tAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAgcgc                                                                                                              >  1:469107/1‑140 (MQ=255)                      tAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAgcgc                                                                                                              >  1:482829/1‑140 (MQ=255)                      tAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAgcgc                                                                                                              >  1:631520/1‑140 (MQ=255)                      tAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAgcgc                                                                                                              >  1:242467/1‑140 (MQ=255)                      tAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAgcgc                                                                                                              >  1:1074929/1‑140 (MQ=255)                         ccGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGcgccgc                                                                                                           >  2:946399/1‑140 (MQ=255)                          cGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTTACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCcgcg                                                                                                          <  1:980567/140‑1 (MQ=255)                            aCAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTCTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGAt                                                                                                        >  2:390247/1‑140 (MQ=255)                             cAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAgcg                                                                                                               <  2:412156/132‑1 (MQ=255)                             cAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAgcg                                                                                                               >  1:412156/1‑132 (MQ=255)                             cAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATg                                                                                                       >  1:207171/1‑140 (MQ=255)                               aTACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAg                                                                                                     >  2:914752/1‑140 (MQ=255)                                tACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGt                                                                                                    >  1:272286/1‑140 (MQ=255)                                       agaATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCt                                                                                             >  1:778666/1‑140 (MQ=255)                                          aTCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTTCTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGaa                                                                                          <  1:1188809/140‑1 (MQ=255)                                          aTCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGaa                                                                                          >  2:86650/1‑140 (MQ=255)                                                 ttAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGa                                                                                   >  2:48917/1‑140 (MQ=255)                                                 ttAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTCTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGa                                                                                   <  1:390247/140‑1 (MQ=255)                                                   aaCGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATCCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAg                                                                                 >  1:1293808/1‑140 (MQ=255)                                                        tGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTcc                                                                            >  1:627159/1‑140 (MQ=255)                                                         ggTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTc                                                                             <  2:978696/138‑1 (MQ=255)                                                         ggTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTc                                                                             >  1:978696/1‑138 (MQ=255)                                                          gTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTccgc                                                                          <  2:1074929/140‑1 (MQ=255)                                                          gTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTccgc                                                                          <  2:482829/140‑1 (MQ=255)                                                          gTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTccgc                                                                          <  2:242467/140‑1 (MQ=255)                                                            cAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTccgccg                                                                        <  2:1231038/140‑1 (MQ=255)                                                                  tGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTAc                                                                  <  1:86650/140‑1 (MQ=255)                                                                    agaAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACca                                                                >  2:704038/1‑140 (MQ=255)                                                                    agaAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACca                                                                >  2:458174/1‑140 (MQ=255)                                                                    agaAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACca                                                                >  2:382771/1‑140 (MQ=255)                                                                        aaaGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACcacaca                                                            <  2:272286/140‑1 (MQ=255)                                                                         aaGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTccgc                                                                          >  1:535837/1‑125 (MQ=255)                                                                         aaGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTccgc                                                                          <  2:535837/125‑1 (MQ=255)                                                                         aaGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGttt                                                                     >  1:796855/1‑130 (MQ=255)                                                                         aaGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGttt                                                                     <  2:796855/130‑1 (MQ=255)                                                                         aaGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACcaca                                                              >  2:1441958/1‑137 (MQ=255)                                                                         aaGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACcaca                                                              <  2:500227/137‑1 (MQ=255)                                                                         aaGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACcaca                                                              <  1:1441958/137‑1 (MQ=255)                                                                         aaGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACcaca                                                              >  1:500227/1‑137 (MQ=255)                                                                            gCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATccc                                                        <  2:631520/140‑1 (MQ=255)                                                                                         aGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGag                                           <  2:627159/140‑1 (MQ=255)                                                                                         aGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGag                                           <  2:402302/140‑1 (MQ=255)                                                                                          gCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGagc                                          >  1:1168596/1‑140 (MQ=255)                                                                                                aaCATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGt                                    >  2:827982/1‑140 (MQ=255)                                                                                                 aCATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGtt                                   <  1:458174/140‑1 (MQ=255)                                                                                                     cGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCt                               >  1:186390/1‑140 (MQ=255)                                                                                                     cGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCt                               >  1:529061/1‑140 (MQ=255)                                                                                                     cGTGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCt                               >  2:208435/1‑140 (MQ=255)                                                                                                       tGGTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGc                             >  1:446920/1‑140 (MQ=255)                                                                                                        ggTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGcc                            >  2:778741/1‑140 (MQ=255)                                                                                                        ggTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGc                             <  2:651111/139‑1 (MQ=255)                                                                                                        ggTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGc                             >  1:651111/1‑139 (MQ=255)                                                                                                         gTTGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCg                           <  1:827982/140‑1 (MQ=255)                                                                                                          ttGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCgg                          <  2:1168596/140‑1 (MQ=255)                                                                                                          ttGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCgg                          <  2:952022/140‑1 (MQ=255)                                                                                                          ttGATGATGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCgg                          <  2:684816/140‑1 (MQ=255)                                                                                                            gatgatGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGcc                            >  2:387678/1‑136 (MQ=255)                                                                                                            gatgatGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGcc                            <  1:387678/136‑1 (MQ=255)                                                                                                            gatgatGCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAg                        <  1:407844/140‑1 (MQ=255)                                                                                                             atgatgCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGt                       <  2:469107/140‑1 (MQ=255)                                                                                                                 tgCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAg                        <  2:1327518/135‑1 (MQ=255)                                                                                                                 tgCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAg                        >  1:1327518/1‑135 (MQ=255)                                                                                                                 tgCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACgg                   >  2:1142705/1‑140 (MQ=255)                                                                                                                  gCCGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGa                  <  2:186390/140‑1 (MQ=255)                                                                                                                   ccGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGAt                 <  1:704038/140‑1 (MQ=255)                                                                                                                    cGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGa                         <  1:576359/131‑1 (MQ=255)                                                                                                                    cGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGa                         >  2:576359/1‑131 (MQ=255)                                                                                                                    cGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGAt                 >  2:282831/1‑139 (MQ=255)                                                                                                                    cGTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGAt                 <  1:282831/139‑1 (MQ=255)                                                                                                                     gTATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAg               <  2:446920/140‑1 (MQ=255)                                                                                                                      tATTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGt              <  1:335507/140‑1 (MQ=255)                                                                                                                       aTTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGcc                            >  2:730119/1‑125 (MQ=255)                                                                                                                       aTTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGcc                            <  1:730119/125‑1 (MQ=255)                                                                                                                       aTTCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGtt             >  1:321748/1‑140 (MQ=255)                                                                                                                        ttCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGa                         >  1:1190778/1‑127 (MQ=255)                                                                                                                        ttCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGa                         <  2:1190778/127‑1 (MQ=255)                                                                                                                        ttCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGttt            >  2:513336/1‑140 (MQ=255)                                                                                                                        ttCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGttt            >  1:466383/1‑140 (MQ=255)                                                                                                                        ttCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGttt            >  2:1081837/1‑140 (MQ=255)                                                                                                                        ttCACGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGttt            >  2:1171203/1‑140 (MQ=255)                                                                                                                           aCGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGTTTGtt         >  1:783723/1‑140 (MQ=255)                                                                                                                           aCGCGCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGTTTGtt         >  2:172828/1‑140 (MQ=255)                                                                                                                            cgcgCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCgg                          >  2:631112/1‑122 (MQ=255)                                                                                                                            cgcgCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCgg                          <  1:631112/122‑1 (MQ=255)                                                                                                                            cgcgCTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGTTTGTTc        <  1:778741/140‑1 (MQ=255)                                                                                                                                cTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGTTTGTTCACAt    >  1:304956/1‑140 (MQ=255)                                                                                                                                cTTTTACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGTTTGTTCACAt    >  1:584650/1‑140 (MQ=255)                                                                                                                                 ttttACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGTTTGTTCACAtt   <  1:208435/140‑1 (MQ=255)                                                                                                                                  tttACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGTTTGt          >  2:456688/1‑132 (MQ=255)                                                                                                                                  tttACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGTTTGt          <  1:456688/132‑1 (MQ=255)                                                                                                                                  tttACTGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGTTTGTTCACATTg  <  2:321748/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       AGGGCAATCGCTTCACGAGCGTAGCCGACAATACGCGGAGAATCGAAGTTAACGTTGGTCAGGGTTGAGAAAAAGGCACGTGGCGCGAAGCTGTCAACATCGTGGTTGATGATGCCGTATTCACGCGCTTTTACCGCCCAGGCAGAAAGCCCTTGCAGCGCCGCGATGAGTAAATCCTGAAGGTCAGAAGTTTCCGCCGTTTTACCACACATCCCCTGCGCGTATGAGCAGCCGTTTCCTGCCGGAGTACGGATAGTTTGTTCACATTG  >  NC_000913/913534‑913802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑