Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	955,255	A→G	Y460H (TAC→CAC)	ycaO ←	ribosomal protein S12 methylthiotransferase accessory factor YcaO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	955,255	0	A	G	100.0%	279.8 / NA	81	Y460H (TAC→CAC)	ycaO	ribosomal protein S12 methylthiotransferase accessory factor YcaO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/42);  total (39/42)

GTTGGCGCGTTCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTG  >  NC_000913/955127‑955380                                                                                                                                 |                                                                                                                              gTTGGCGCGTTCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGcc                                                                                                                             >  2:562452/1‑131 (MQ=255)   tGGCGCGTTCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATcc                                                                                                                  >  1:1264060/1‑140 (MQ=255)   tGGCGCGTTCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATcc                                                                                                                  >  2:217390/1‑140 (MQ=255)    ggCGCGTTCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCg                                                                                                                 >  2:253085/1‑140 (MQ=255)     gcgcGTTCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGa                                                                                                                >  1:596410/1‑140 (MQ=255)      cgcgTTCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGAc                                                                                                               >  2:993035/1‑140 (MQ=255)      cgcgTTCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGAc                                                                                                               >  2:808888/1‑140 (MQ=255)      cgcgTTCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGAc                                                                                                               <  2:562299/140‑1 (MQ=255)         gTTCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCg                                                                                                            <  2:969125/140‑1 (MQ=255)           tCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGt                                                                                                          <  1:1233217/140‑1 (MQ=255)             cGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCg                                                                                                        <  2:596410/140‑1 (MQ=255)                 cTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAg                                                                                                    <  1:993035/140‑1 (MQ=255)                    aaTACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGAcc                                                                                                 >  1:112159/1‑140 (MQ=255)                     aTACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCg                                                                                                            <  1:536078/128‑1 (MQ=255)                     aTACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCg                                                                                                            >  2:536078/1‑128 (MQ=255)                       aCTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCaa                                                                                              <  1:732656/140‑1 (MQ=255)                          gatgaGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAg                                                                                           >  1:1298212/1‑140 (MQ=255)                           atgaGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCg                                                                                                        <  2:1082028/126‑1 (MQ=255)                           atgaGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCg                                                                                                        >  1:1082028/1‑126 (MQ=255)                           atgaGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGc                                                                                          >  2:1464593/1‑140 (MQ=255)                            tgaGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCt                                                                                         >  2:1012329/1‑140 (MQ=255)                              aGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTca                                                                                       >  2:1015143/1‑140 (MQ=255)                              aGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTca                                                                                       >  1:193711/1‑140 (MQ=255)                                ttAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGTCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTcacg                                                                                     >  1:271039/1‑140 (MQ=255)                                ttAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCTCCAGACCCAACAGCTcacg                                                                                     >  1:381301/1‑140 (MQ=255)                                ttAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCTCCAGACCCAACAGCTcacg                                                                                     <  2:381301/140‑1 (MQ=255)                                ttAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTcacg                                                                                     <  2:190940/140‑1 (MQ=255)                                ttAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTcacg                                                                                     >  2:990083/1‑140 (MQ=255)                                  aaaCTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTcacgca                                                                                   <  2:1294071/140‑1 (MQ=255)                                  aaaCTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTcacgca                                                                                   <  2:1062868/140‑1 (MQ=255)                                  aaaCTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTcacgca                                                                                   <  2:494964/140‑1 (MQ=255)                                   aaCTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTcacgcac                                                                                  <  2:1015936/140‑1 (MQ=255)                                         aTCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTcacgcacg                                                                                 >  2:237634/1‑135 (MQ=255)                                         aTCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTcacgcacg                                                                                 <  1:237634/135‑1 (MQ=255)                                         aTCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGt                                                                            <  1:322011/140‑1 (MQ=255)                                             tCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAg                                                                        >  1:653785/1‑140 (MQ=255)                                                  tCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGtcatc                                                                   >  2:478700/1‑140 (MQ=255)                                                  tCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGtcatc                                                                   >  1:158599/1‑140 (MQ=255)                                                      tCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCaaaa                                                               <  1:1015143/140‑1 (MQ=255)                                                      tCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCaaaa                                                               >  2:207593/1‑140 (MQ=255)                                                      tCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCaaaa                                                               >  1:232041/1‑140 (MQ=255)                                                       ccagaccagaGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAAc                                                              <  2:653785/140‑1 (MQ=255)                                                          gaccagaGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACctt                                                           <  1:1373262/140‑1 (MQ=255)                                                          gaccagaGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACctt                                                           <  1:1037102/140‑1 (MQ=255)                                                          gaccagaGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACctt                                                           <  2:158599/140‑1 (MQ=255)                                                           accagaGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAAc                                                              >  1:1018286/1‑136 (MQ=255)                                                           accagaGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAAc                                                              <  2:1018286/136‑1 (MQ=255)                                                            ccagaGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACcttct                                                         <  2:736697/140‑1 (MQ=255)                                                               gagCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCa                                                      >  1:13915/1‑140 (MQ=255)                                                                agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCaaa                                                                <  2:1270677/129‑1 (MQ=255)                                                                agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCaaa                                                                >  1:1270677/1‑129 (MQ=255)                                                                agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCAt                                                     >  2:947539/1‑140 (MQ=255)                                                                agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCAt                                                     >  1:109770/1‑140 (MQ=255)                                                                agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCAATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCaaa                                                                >  1:561684/1‑129 (MQ=255)                                                                agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCAATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCaaa                                                                <  2:561684/129‑1 (MQ=255)                                                                 gCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATc                                                    <  1:207593/140‑1 (MQ=255)                                                                     gTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCATTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCGTCAAAACCTTCTTCATCCAGt                                                >  2:1338932/1‑140 (MQ=255)                                                                         cAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCt                                            <  2:1264060/140‑1 (MQ=255)                                                                          aGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTc                                           >  1:216717/1‑140 (MQ=255)                                                                            aTCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCACCCAGTTGCTCGa                                         >  2:451203/1‑140 (MQ=255)                                                                             tCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGAt                                        <  2:498771/140‑1 (MQ=255)                                                                              cGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATg                                       <  1:253085/140‑1 (MQ=255)                                                                              cGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATg                                       <  2:802783/140‑1 (MQ=255)                                                                              cGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATg                                       <  1:808888/140‑1 (MQ=255)                                                                                ccaccaGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAg                                     >  2:741290/1‑140 (MQ=255)                                                                                 caccaGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAgg                                    <  2:109770/140‑1 (MQ=255)                                                                                  accaGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGt                                   >  2:63031/1‑140 (MQ=255)                                                                                   ccagccagCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGtt                                  >  2:255884/1‑140 (MQ=255)                                                                                    cagccagcGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTc                                 <  2:1183253/140‑1 (MQ=255)                                                                                    cagccagcGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTc                                 <  1:395835/140‑1 (MQ=255)                                                                                    cagccagcGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCACCCAGTTGCTCGATGAGGTTc                                 <  1:451203/140‑1 (MQ=255)                                                                                       ccagcgccagcATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAgg                              <  2:13915/140‑1 (MQ=255)                                                                                           cgccagcATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATg                                       <  2:212507/127‑1 (MQ=255)                                                                                           cgccagcATGGCTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATg                                       >  1:212507/1‑127 (MQ=255)                                                                                                      cTTTTAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTcc               <  1:255884/140‑1 (MQ=255)                                                                                                         ttAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCAc            >  2:1106200/1‑140 (MQ=255)                                                                                                          tAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACt           <  2:1298212/140‑1 (MQ=255)                                                                                                          tAATTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACt           <  1:947539/140‑1 (MQ=255)                                                                                                            aTTCACCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCg         <  2:112159/140‑1 (MQ=255)                                                                                                                aCCGATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGc     >  2:1181447/1‑140 (MQ=255)                                                                                                                   gATACGCAGGGTGTGCCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTg  <  1:17994/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                              GTTGGCGCGTTCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTG  >  NC_000913/955127‑955380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑