Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,018,893	T→C	S137P (TCT→CCT)	matP →	macrodomain Ter protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,018,893	0	T	C	100.0%	146.5 / NA	43	S137P (TCT→CCT)	matP	macrodomain Ter protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/23);  total (20/23)

AAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGA  >  NC_000913/1018756‑1019028                                                                                                                                          |                                                                                                                                        aaaaaTCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGcct                                                                                                                                       >  1:87743/1‑140 (MQ=255)      tcgatcgaTCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTctct                                                                                                                                  <  2:1169556/140‑1 (MQ=255)        gatcgatcTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGa                                                                                                                                >  2:133132/1‑140 (MQ=255)        gatcgatcTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGa                                                                                                                                >  1:785182/1‑140 (MQ=255)                       tATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAgg                                                                                                                 <  1:520539/140‑1 (MQ=255)                        aTTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGc                                                                                                                >  2:68448/1‑140 (MQ=255)                        aTTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGc                                                                                                                >  2:198161/1‑140 (MQ=255)                        aTTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGc                                                                                                                >  2:1099094/1‑140 (MQ=255)                            tCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTg                                                                                                            <  2:606024/140‑1 (MQ=255)                            tCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTg                                                                                                            <  1:146177/140‑1 (MQ=255)                            tCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTg                                                                                                            <  1:38982/140‑1 (MQ=255)                             cTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGc                                                                                                           <  2:977362/140‑1 (MQ=255)                                gCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCt                                                                                                                      >  2:295589/1‑126 (MQ=255)                                gCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCt                                                                                                                      <  1:295589/126‑1 (MQ=255)                                 cAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTggg                                                                                                       <  1:198161/140‑1 (MQ=255)                                  aaCGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGt                                                                                                      <  1:1180723/140‑1 (MQ=255)                                      tCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAgg                                                                                                  >  1:1442339/1‑140 (MQ=255)                                      tCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAgg                                                                                                  >  2:1346780/1‑140 (MQ=255)                                        tggctggTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGaa                                                                                                <  1:691279/140‑1 (MQ=255)                                          gctggTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATa                                                                                              >  1:159172/1‑140 (MQ=255)                                           ctggTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATaa                                                                                             <  1:615831/140‑1 (MQ=255)                                           ctggTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATaa                                                                                             >  1:513717/1‑140 (MQ=255)                                                    cgcAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGaaa                                                                                    >  1:174226/1‑140 (MQ=255)                                                      cAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGt                                                                                  <  2:1442339/140‑1 (MQ=255)                                                         cGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAg                                                                               >  2:455024/1‑140 (MQ=255)                                                         cGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAg                                                                               >  1:893621/1‑140 (MQ=255)                                                          gTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGt                                                                              <  1:429472/140‑1 (MQ=255)                                                           tCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGtt                                                                             <  2:66083/140‑1 (MQ=255)                                                                          ttCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGc                                                              <  2:159172/140‑1 (MQ=255)                                                                                    aTTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAAcc                                                    >  2:143318/1‑140 (MQ=255)                                                                                     ttGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAAccc                                                   >  1:975343/1‑140 (MQ=255)                                                                                                aTTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGGAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCgg                                        >  1:852228/1‑140 (MQ=255)                                                                                                aTTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCgg                                        >  1:1466605/1‑140 (MQ=255)                                                                                                aTTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAACTTACTAAAGGCAAAAAAAACCCCGCAGCAGCgg                                        <  2:174226/140‑1 (MQ=255)                                                                                                 ttGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCggg                                       <  1:143318/140‑1 (MQ=255)                                                                                                          cGGAAAACAAAGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTa                              >  2:437239/1‑140 (MQ=255)                                                                                                                  aaaGAGAAATACGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACga                      <  2:335837/140‑1 (MQ=255)                                                                                                                            aCGCGAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGc            >  1:907889/1‑140 (MQ=255)                                                                                                                               cgAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTg         >  1:560005/1‑140 (MQ=255)                                                                                                                                gAATAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGc        <  2:1466605/140‑1 (MQ=255)                                                                                                                                  aTAAAATGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCgg      <  2:1410631/140‑1 (MQ=255)                                                                                                                                    aaaaTGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCt    <  1:68448/140‑1 (MQ=255)                                                                                                                                    aaaaTGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCt    <  1:820921/140‑1 (MQ=255)                                                                                                                                      aaTGCCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGa  <  1:1099094/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        AAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGA  >  NC_000913/1018756‑1019028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑