Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,035,301	A→G	Y79C (TAC→TGC)	hyaC →	hydrogenase 1 cytochrome b subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,035,301	0	A	G	100.0%	320.3 / NA	93	Y79C (TAC→TGC)	hyaC	hydrogenase 1 cytochrome b subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (52/41);  total (52/41)

CACCGGATACTTTATCGGCAAGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTACTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTCCGAGTGCCGATATAGGC  >  NC_000913/1035167‑1035437                                                                                                                                       |                                                                                                                                         cACCGGATACTTTATCGGCAAGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTggg                                                                                                                                     >  2:780046/1‑140 (MQ=255)  aCCGGATACTTTATCGGCAAGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGc                                                                                                                                    >  1:225089/1‑140 (MQ=255)   ccGGATACTTTATCGGCAAGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCt                                                                                                                                   >  2:796422/1‑140 (MQ=255)      gATACTTTATCGGCAAGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCtttt                                                                                                                                >  2:436848/1‑140 (MQ=255)        tACTTTATCGGCAAGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTttgt                                                                                                                              <  2:1154003/140‑1 (MQ=255)         aCTTTATCGGCAAGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTttgtt                                                                                                                             >  2:366104/1‑140 (MQ=255)              aTCGGCAAGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCaa                                                                                                                        >  1:415953/1‑140 (MQ=255)              aTCGGCAAGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCaa                                                                                                                        >  2:1004632/1‑140 (MQ=255)               tCGGCAAGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAAt                                                                                                                       <  2:147744/140‑1 (MQ=255)                    aaGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATa                                                                                                                  <  2:665402/140‑1 (MQ=255)                     aGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGTGGATCTGCTGGGCTTTTGTTGGCAATCGATAc                                                                                                                 >  1:287583/1‑140 (MQ=255)                     aGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAAt                                                                                                                       >  1:537446/1‑134 (MQ=255)                     aGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAAt                                                                                                                       <  2:537446/134‑1 (MQ=255)                      gCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACt                                                                                                                >  1:524060/1‑140 (MQ=255)                           tACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCcgc                                                                                                           <  2:540372/140‑1 (MQ=255)                            aCCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCcgcg                                                                                                          <  1:796422/140‑1 (MQ=255)                            aCCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCcgcg                                                                                                          <  2:225089/140‑1 (MQ=255)                              cTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAg                                                                                                        <  1:78388/140‑1 (MQ=255)                                     cAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTa                                                                                                 <  1:780046/140‑1 (MQ=255)                                      aGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGCTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTAt                                                                                                <  1:779901/140‑1 (MQ=255)                                           cgaggcgaCGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCg                                                                                              <  2:524617/137‑1 (MQ=255)                                           cgaggcgaCGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCg                                                                                              >  1:524617/1‑137 (MQ=255)                                                cgaCGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTa                                                                                      <  1:1203106/140‑1 (MQ=255)                                                 gaCGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTAt                                                                                     <  2:1467655/140‑1 (MQ=255)                                                              tataTGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTa                                                                                      >  2:710285/1‑126 (MQ=255)                                                              tataTGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTa                                                                                      <  1:710285/126‑1 (MQ=255)                                                              tataTGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCtg                                                                        >  2:1428988/1‑140 (MQ=255)                                                              tataTGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCtg                                                                        >  2:865041/1‑140 (MQ=255)                                                               atatGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGc                                                                          <  2:298485/137‑1 (MQ=255)                                                               atatGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGc                                                                          >  1:298485/1‑137 (MQ=255)                                                                tatGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCtggt                                                                      >  2:42861/1‑140 (MQ=255)                                                                tatGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCtggt                                                                      >  2:738340/1‑140 (MQ=255)                                                                tatGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCtggt                                                                      >  1:348382/1‑140 (MQ=255)                                                                  tGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGGGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCtggtgg                                                                    >  1:366976/1‑140 (MQ=255)                                                                   gggCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGc                                                                   <  1:436848/140‑1 (MQ=255)                                                                    ggCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCa                                                                  >  2:1244520/1‑140 (MQ=255)                                                                     gCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAg                                                                 >  2:1070631/1‑140 (MQ=255)                                                                     gCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAg                                                                 >  2:66344/1‑140 (MQ=255)                                                                     gCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAg                                                                 >  2:421872/1‑140 (MQ=255)                                                                     gCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAg                                                                 >  1:8469/1‑140 (MQ=255)                                                                     gCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAg                                                                 >  1:20936/1‑140 (MQ=255)                                                                     gCTACATCAGGTTAATTCACTTCAGCGCCGGGATGG‑TTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAgg                                                                >  1:1408964/1‑140 (MQ=255)                                                                       tACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAggg                                                               <  2:348382/140‑1 (MQ=255)                                                                        aCATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGc                                                                          <  1:913553/128‑1 (MQ=255)                                                                        aCATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGc                                                                          >  2:913553/1‑128 (MQ=255)                                                                        aCATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGc                                                              <  1:225153/140‑1 (MQ=255)                                                                        aCATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGc                                                              >  1:1008869/1‑140 (MQ=255)                                                                          aTCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCgt                                                            >  1:513922/1‑140 (MQ=255)                                                                          aTCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCgt                                                            <  2:524060/140‑1 (MQ=255)                                                                          aTCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCgt                                                            <  1:922580/140‑1 (MQ=255)                                                                          aTCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCgt                                                            >  1:577004/1‑140 (MQ=255)                                                                          aTCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCgt                                                            >  2:239574/1‑140 (MQ=255)                                                                           tCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCgtg                                                           <  1:42861/140‑1 (MQ=255)                                                                            cAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCgtgt                                                          <  2:415953/140‑1 (MQ=255)                                                                              ggTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTgg                                                        >  2:809791/1‑140 (MQ=255)                                                                               gTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGt                                                       >  2:378433/1‑140 (MQ=255)                                                                               gTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGt                                                       >  1:525580/1‑140 (MQ=255)                                                                               gTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGt                                                       >  1:1291750/1‑140 (MQ=255)                                                                                   aTTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGa                                                   >  2:114475/1‑140 (MQ=255)                                                                                   aTTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGa                                                   >  2:151725/1‑140 (MQ=255)                                                                                    ttCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAGAGCTGGTGGCAGGGCGTGTGGTATGaa                                                  >  2:639279/1‑140 (MQ=255)                                                                                    ttCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGaa                                                  >  2:127144/1‑140 (MQ=255)                                                                                      cACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGtgtg                                                         >  2:510480/1‑131 (MQ=255)                                                                                      cACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGtgtg                                                         <  1:510480/131‑1 (MQ=255)                                                                                      cACTTCAGCGCCGGGATGG‑TTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATc                                               <  2:1408964/140‑1 (MQ=255)                                                                                         ttCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCgtgt                                                          >  1:399371/1‑127 (MQ=255)                                                                                         ttCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCgtgt                                                          <  2:399371/127‑1 (MQ=255)                                                                                         ttCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCg                                             >  2:555196/1‑140 (MQ=255)                                                                                          tCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGc                                            <  1:1428988/140‑1 (MQ=255)                                                                                           cAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCt                                           <  2:726520/140‑1 (MQ=255)                                                                                           cAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCt                                           <  1:114475/140‑1 (MQ=255)                                                                                            aGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAACTCCGCTg                                          >  1:674752/1‑140 (MQ=255)                                                                                              cgcCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGt                                        >  2:121094/1‑140 (MQ=255)                                                                                              cgcCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCCGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGt                                        >  2:925985/1‑140 (MQ=255)                                                                                                ccGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTAt                                      <  1:121094/140‑1 (MQ=255)                                                                                                ccGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTAt                                      <  2:513922/140‑1 (MQ=255)                                                                                                ccGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTAt                                      <  2:8469/140‑1 (MQ=255)                                                                                                 cGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATc                                     >  2:1274430/1‑140 (MQ=255)                                                                                                      tGGTTTTTACCGTGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGttt                                <  2:1291750/140‑1 (MQ=255)                                                                                                                  tGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGt                    >  2:1195920/1‑140 (MQ=255)                                                                                                                  tGGTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGt                    >  2:282360/1‑140 (MQ=255)                                                                                                                   ggTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTc                   >  1:975999/1‑140 (MQ=255)                                                                                                                   ggTTTTGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTc                   >  1:1378611/1‑140 (MQ=255)                                                                                                                     ttttGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTCCg                 <  1:282360/140‑1 (MQ=255)                                                                                                                     ttttGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTCCg                 <  2:103339/140‑1 (MQ=255)                                                                                                                     ttttGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTCCg                 <  1:1244520/140‑1 (MQ=255)                                                                                                                     ttttGCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTCCg                 <  2:171369/140‑1 (MQ=255)                                                                                                                         gCTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTCCGAGTg             <  1:412583/140‑1 (MQ=255)                                                                                                                          cTGATGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTCCGAGTGc            <  2:20936/140‑1 (MQ=255)                                                                                                                             aTGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTCCGAGTGCCGa         >  1:686843/1‑140 (MQ=255)                                                                                                                              tGCGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTCCGAGTGCCGat        >  2:108819/1‑140 (MQ=255)                                                                                                                                cGGATCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTCCGAGTGCCGatat      <  1:555196/140‑1 (MQ=255)                                                                                                                                    tCTGCTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTCCGAGTGCCGATATAGGc  <  2:1008869/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CACCGGATACTTTATCGGCAAGCCGCTACCTTCCGTCAGCGGCGAGGCGACGTATCTGTTCTATATGGGCTACATCAGGTTAATTCACTTCAGCGCCGGGATGGTTTTTACCGTGGTTTTGCTGATGCGGATCTACTGGGCTTTTGTTGGCAATCGATACTCCCGCGAGCTGTTTATCGTGCCGGTATGGCGTAAAAGCTGGTGGCAGGGCGTGTGGTATGAAATCCGCTGGTATCTGTTTCTGGCAAAACGTCCGAGTGCCGATATAGGC  >  NC_000913/1035167‑1035437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑