Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,527	G→A	A174A (GCC→GCT)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,527	0	G	A	100.0%	206.1 / NA	58	A174A (GCC→GCT)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/32);  total (26/32)

GGCGGCAGGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTC  >  NC_000913/69393‑69661                                                                                                                                       |                                                                                                                                       ggcggcAGGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGa                                                                                                                                   <  1:583170/140‑1 (MQ=255)      cAGGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTgcg                                                                                                                              <  1:687756/140‑1 (MQ=255)        ggcagccCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGcc                                                                                                                            >  1:1215977/5‑140 (MQ=255)              cccAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGgcg                                                                                                                      <  2:1351011/140‑1 (MQ=255)              cccAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGgcg                                                                                                                      <  2:1194045/140‑1 (MQ=255)              cccAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGgcg                                                                                                                      <  1:614527/140‑1 (MQ=255)              cccAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGgcg                                                                                                                      <  1:1191750/140‑1 (MQ=255)              cccAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGgcg                                                                                                                      <  1:184610/140‑1 (MQ=255)                cAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCt                                                                                                                    <  2:945075/140‑1 (MQ=255)                   cTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCt                                                                                                                    >  1:985326/1‑137 (MQ=255)                   cTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCt                                                                                                                    <  2:985326/137‑1 (MQ=255)                       cGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTc                                                                                                                 >  1:91132/1‑136 (MQ=255)                       cGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTc                                                                                                                 <  2:91132/136‑1 (MQ=255)                        gTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCa                                                                                                                           <  2:536675/125‑1 (MQ=255)                        gTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCa                                                                                                                           >  1:536675/1‑125 (MQ=255)                        gTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGgcg                                                                                                                      >  2:713549/1‑130 (MQ=255)                        gTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGgcg                                                                                                                      <  1:713549/130‑1 (MQ=255)                        gTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGc                                                                                                            <  1:1154339/140‑1 (MQ=255)                              caGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTTGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTca                                                                                                      <  2:76190/140‑1 (MQ=255)                                   aTTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGc                                                                                                 >  1:1350279/1‑140 (MQ=255)                                             ggCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTg                                                                                       >  1:1139601/1‑140 (MQ=255)                                             ggCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTg                                                                                       >  1:1353370/1‑140 (MQ=255)                                                    cAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGAttt                                                                                          >  2:383743/1‑130 (MQ=255)                                                    cAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGAttt                                                                                          <  1:383743/130‑1 (MQ=255)                                                    cAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGaa                                                                                >  2:798995/1‑140 (MQ=255)                                                           cgcgACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCg                                                                         <  1:423035/140‑1 (MQ=255)                                                                      tCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGaataaat                                                              <  2:1350279/140‑1 (MQ=255)                                                                      tCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGaataaat                                                              <  2:86592/140‑1 (MQ=255)                                                                          gcgggcggGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGTGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATacca                                                          >  2:593437/1‑140 (MQ=255)                                                                          gcgggcggGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATacca                                                          >  1:399366/1‑140 (MQ=255)                                                                           cgggcgggTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATaccac                                                         >  1:755278/1‑140 (MQ=255)                                                                            gggcgggTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATaccacc                                                        >  1:1252093/1‑140 (MQ=255)                                                                              gcgggTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCCGGAATAAATACCACCaa                                                      <  1:1053240/140‑1 (MQ=255)                                                                                   tggtACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAg                                                 <  2:1139601/140‑1 (MQ=255)                                                                                   tggtACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAg                                                 <  2:1252093/140‑1 (MQ=255)                                                                                   tggtACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAg                                                 <  2:1353370/140‑1 (MQ=255)                                                                                        ccGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGa                                            <  2:1203827/140‑1 (MQ=255)                                                                                           gAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGagta                                         >  2:1432199/1‑140 (MQ=255)                                                                                            aaaGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGagtag                                        >  1:210848/1‑140 (MQ=255)                                                                                             aaGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGagtagt                                       >  2:525204/1‑140 (MQ=255)                                                                                                cAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCaa                                    >  1:1037279/1‑140 (MQ=255)                                                                                                        gCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGccc                            >  2:1137917/1‑140 (MQ=255)                                                                                                        gCACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGccc                            >  2:926974/1‑140 (MQ=255)                                                                                                         cACCCAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCAATCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCg                           <  2:1179535/140‑1 (MQ=255)                                                                                                            ccAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGgcg                        <  2:245145/140‑1 (MQ=255)                                                                                                             cAGTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGgcgc                       >  2:14975/1‑140 (MQ=255)                                                                                                               gTCGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGt                     >  1:65379/1‑140 (MQ=255)                                                                                                                 cGCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTgg                   <  1:525204/140‑1 (MQ=255)                                                                                                                  gCACAGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGc                  >  1:1143427/1‑140 (MQ=255)                                                                                                                   cacaGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGca                 <  2:210848/140‑1 (MQ=255)                                                                                                                      aGCTCAATCCACGATGCAGCAGATTGTGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACaa              <  1:593437/140‑1 (MQ=255)                                                                                                                      aGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACaa              <  2:112198/140‑1 (MQ=255)                                                                                                                      aGCTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACaa              <  2:65379/140‑1 (MQ=255)                                                                                                                        cTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAAc            <  2:1037279/140‑1 (MQ=255)                                                                                                                        cTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAAc            <  1:798995/140‑1 (MQ=255)                                                                                                                        cTCAATCCACGATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAAc            >  1:594803/1‑140 (MQ=255)                                                                                                                                  gATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAAc            <  2:1047761/130‑1 (MQ=255)                                                                                                                                  gATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAAc            >  1:1047761/1‑130 (MQ=255)                                                                                                                                  gATGCAGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAACACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAAtctc  >  1:1126513/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       GGCGGCAGGCCGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTC  >  NC_000913/69393‑69661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑