Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,241,823	A→G	S83S (AGT→AGC)	cvrA ←	putative K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,241,823	0	A	G	100.0%	234.6 / NA	68	S83S (AGT→AGC)	cvrA	putative K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/38);  total (30/38)

ACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGACTCCTGCCAGCATGCCGATCGCCAGA  >  NC_000913/1241686‑1241958                                                                                                                                          |                                                                                                                                        acgaTAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCt                                                                                                                                       >  1:85755/1‑140 (MQ=255)      aGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCt                                                                                                                                  <  1:1108441/140‑1 (MQ=255)       gcgcCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTg                                                                                                                                 <  2:1401584/140‑1 (MQ=255)        cgcCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGa                                                                                                                                <  2:163229/140‑1 (MQ=255)          ccGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGt                                                                                                                              <  2:824552/140‑1 (MQ=255)           cGATTAATAAGCCTTTAATCAACTCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCGAGCGACAGTGCAAGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTg                                                                                                                             <  2:350637/140‑1 (MQ=255)             aTTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTgcg                                                                                                                           >  1:10020/1‑140 (MQ=255)             aTTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTgcg                                                                                                                           >  1:820638/1‑140 (MQ=255)                       cTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCccgccg                                                                                                                 >  1:773456/1‑140 (MQ=255)                          caatcaaATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCg                                                                                                              >  2:759037/1‑140 (MQ=255)                                     aGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAaataat                                                                                                   >  2:954172/1‑140 (MQ=255)                                       aTTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCg                                                                                                 >  2:847850/1‑140 (MQ=255)                                        ttAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGc                                                                                                <  1:813852/140‑1 (MQ=255)                                        ttAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGc                                                                                                <  2:567795/140‑1 (MQ=255)                                        ttAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGc                                                                                                <  2:773456/140‑1 (MQ=255)                                        ttAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGc                                                                                                <  2:85755/140‑1 (MQ=255)                                           aaCAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAg                                                                                             >  1:581534/1‑140 (MQ=255)                                             cAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTg                                                                                           <  1:278037/140‑1 (MQ=255)                                             cAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTg                                                                                           <  2:1228641/140‑1 (MQ=255)                                               gCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGcc                                                                                         <  1:156669/140‑1 (MQ=255)                                                ccACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGTCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCa                                                                                        <  2:1281938/140‑1 (MQ=255)                                                ccACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCa                                                                                        >  2:852559/1‑140 (MQ=255)                                                ccACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCa                                                                                        >  1:1419526/1‑140 (MQ=255)                                                 cACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTg                                                                                           >  2:958433/1‑136 (MQ=255)                                                 cACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTg                                                                                           <  1:958433/136‑1 (MQ=255)                                                 cACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGcc                                                                                         <  1:113192/138‑1 (MQ=255)                                                 cACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGcc                                                                                         >  2:113192/1‑138 (MQ=255)                                                 cACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAg                                                                                       <  1:1439100/140‑1 (MQ=255)                                                  aCGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAgg                                                                                      <  1:719406/140‑1 (MQ=255)                                                  aCGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAgg                                                                                      <  2:1217975/140‑1 (MQ=255)                                                   cgccgcCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGt                                                                                     <  1:8984/140‑1 (MQ=255)                                                   cgccgcCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGt                                                                                     <  1:1188954/140‑1 (MQ=255)                                                   cgccgcCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGt                                                                                     <  2:702109/140‑1 (MQ=255)                                                   cgccgcCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGt                                                                                     <  1:759037/140‑1 (MQ=255)                                                          atcatGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGttacttac                                                                              >  1:1036345/1‑140 (MQ=255)                                                           tcatGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTAcc                                                                             <  2:1419526/140‑1 (MQ=255)                                                              tGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAATATAATCGCCAGTGCCAGGTTACTTACCATg                                                                          >  2:900183/1‑140 (MQ=255)                                                              tGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATg                                                                          >  1:1104443/1‑140 (MQ=255)                                                                  ggTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAgg                                                                      >  2:84112/1‑140 (MQ=255)                                                                  ggTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAgg                                                                      <  2:1469/140‑1 (MQ=255)                                                                   gTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGttactta                                                                               >  2:1408622/1‑130 (MQ=255)                                                                   gTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGttactta                                                                               <  1:1408622/130‑1 (MQ=255)                                                                     tAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGa                                                                   <  2:581534/140‑1 (MQ=255)                                                                     tAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGa                                                                   <  2:665264/140‑1 (MQ=255)                                                                     tAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGa                                                                   <  1:84112/140‑1 (MQ=255)                                                                       aaCCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAg                                                                 <  1:847850/140‑1 (MQ=255)                                                                             agGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTaa                                                           >  1:956939/1‑140 (MQ=255)                                                                             agGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTaa                                                           <  1:900183/140‑1 (MQ=255)                                                                                tGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTa                                                        <  2:1005884/140‑1 (MQ=255)                                                                                  aTAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATc                                                      >  2:1434353/1‑140 (MQ=255)                                                                                  aTAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATc                                                      >  1:594377/1‑140 (MQ=255)                                                                                       cACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACg                                                 >  1:285148/1‑140 (MQ=255)                                                                                          gCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGa                                              >  1:1161910/1‑140 (MQ=255)                                                                                                    gCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGAc                                    <  1:954172/140‑1 (MQ=255)                                                                                                         cGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCAt                               >  2:1277005/1‑140 (MQ=255)                                                                                                         cGACAGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCAt                               <  2:1104443/140‑1 (MQ=255)                                                                                                             aGTGCCGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGAc                           >  2:914242/1‑140 (MQ=255)                                                                                                                  cGGTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGACTCCTg                      <  2:285148/140‑1 (MQ=255)                                                                                                                   ggTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGACTCCTGc                     <  2:594377/140‑1 (MQ=255)                                                                                                                   ggTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGCTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGACTCCTGc                     <  1:1285808/140‑1 (MQ=255)                                                                                                                    gTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGAc                                    <  2:220508/124‑1 (MQ=255)                                                                                                                    gTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGACTCCTGcc                    >  2:668410/1‑140 (MQ=255)                                                                                                                    gTCCTAACGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGCAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGAc                                    >  1:220508/1‑124 (MQ=255)                                                                                                                         aaCGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGa                            >  2:1035113/1‑127 (MQ=255)                                                                                                                         aaCGCCACACGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGa                            <  1:1035113/127‑1 (MQ=255)                                                                                                                               acacGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGACTCCTGCCAg                  <  1:218849/131‑1 (MQ=255)                                                                                                                               acacGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGACTCCTGCCAg                  >  2:218849/1‑131 (MQ=255)                                                                                                                               acacGAAAGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGACTCCTGCCAGCATGCCGAt         >  1:455618/1‑140 (MQ=255)                                                                                                                                      aGGAGCTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGACTCCTGCCAGCATGCCGATCGCCAGa  >  1:489583/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        ACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACGCCATCGACTCCTGCCAGCATGCCGATCGCCAGA  >  NC_000913/1241686‑1241958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑