Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,271,969	A→G	intergenic (‑120/‑150)	chaA ← / → chaB	Na(+)/K(+):H(+) antiporter ChaA/putative cation transport regulator ChaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,271,969	0	A	G	100.0%	168.6 / NA	49	intergenic (‑120/‑150)	chaA/chaB	Na(+)/K(+):H(+) antiporter ChaA/putative cation transport regulator ChaB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/25);  total (24/25)

CTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGACCACACTTAATGTTCAACTT  >  NC_000913/1271832‑1272101                                                                                                                                          |                                                                                                                                     ctctTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCt                                                                                                                                    >  1:488152/1‑140 (MQ=255)                  ttATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAAt                                                                                                                   <  2:167459/140‑1 (MQ=255)                        ttATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTAt                                                                                                             <  2:531952/140‑1 (MQ=255)                        ttATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTAt                                                                                                             <  2:234654/140‑1 (MQ=255)                        ttATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTAt                                                                                                             <  2:1255994/140‑1 (MQ=255)                          aTCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTa                                                                                                           >  1:818493/1‑140 (MQ=255)                          aTCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTa                                                                                                           >  1:423492/1‑140 (MQ=255)                           tCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTAc                                                                                                          >  2:1023875/1‑140 (MQ=255)                              cTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGtt                                                                                                       <  1:205345/140‑1 (MQ=255)                               tttGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGttt                                                                                                      >  1:103494/1‑140 (MQ=255)                                 tGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAAt                                                                                                                   >  1:981033/1‑125 (MQ=255)                                 tGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAAt                                                                                                                   <  2:981033/125‑1 (MQ=255)                                 tGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAg                                                                                                    >  2:972943/1‑140 (MQ=255)                                 tGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAg                                                                                                    >  1:1263562/1‑140 (MQ=255)                                 tGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAg                                                                                                    >  1:923688/1‑140 (MQ=255)                                    aGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACa                                                                                                 <  2:1284684/140‑1 (MQ=255)                                      aTGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAAt                                                                                               >  2:138240/1‑140 (MQ=255)                                        gAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAAtgt                                                                                             <  2:923688/140‑1 (MQ=255)                                           tttATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAAtgtttg                                                                                          >  1:630495/1‑140 (MQ=255)                                              aTCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATgtttgttt                                                                                       >  2:1174577/1‑140 (MQ=255)                                                  aaaaTGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGc                                                                                   <  2:1263562/140‑1 (MQ=255)                                                   aaaTGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCg                                                                                  >  2:1379922/1‑140 (MQ=255)                                                    aaTGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGt                                                                                 <  1:1023875/140‑1 (MQ=255)                                                    aaTGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGt                                                                                 <  2:423492/140‑1 (MQ=255)                                                    aaTGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGt                                                                                 <  1:1292322/140‑1 (MQ=255)                                                    aaTGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGt                                                                                 <  1:517699/140‑1 (MQ=255)                                                    aaTGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGt                                                                                 <  1:965866/140‑1 (MQ=255)                                                      tGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGtct                                                                               <  1:1174577/140‑1 (MQ=255)                                                             aaTAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTaaga                                                                        <  2:1111197/140‑1 (MQ=255)                                                             aaTAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTaaga                                                                        <  1:578310/140‑1 (MQ=255)                                                                            tAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGaa                                                         >  1:1148696/1‑140 (MQ=255)                                                                                  aaaGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCt                                                   >  2:633357/1‑140 (MQ=255)                                                                                      tgtCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATg                                               >  1:681024/1‑140 (MQ=255)                                                                                            gATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTgg                                         <  1:868313/140‑1 (MQ=255)                                                                                             atatCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGc                                        <  1:1379922/140‑1 (MQ=255)                                                                                             atatCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGc                                        <  2:103494/140‑1 (MQ=255)                                                                                             atatCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGc                                        <  1:972943/140‑1 (MQ=255)                                                                                                tCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAag                                     >  2:439007/1‑140 (MQ=255)                                                                                                  ggTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAagag                                   >  1:667324/1‑140 (MQ=255)                                                                                                       cATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGa                               <  2:220627/139‑1 (MQ=255)                                                                                                       cATTTTACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGa                               >  1:220627/1‑139 (MQ=255)                                                                                                          tttACGAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATAtt                           >  1:1023656/1‑140 (MQ=255)                                                                                                               gAATTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGa                      <  2:818493/140‑1 (MQ=255)                                                                                                                aaTTTTTACCCCGCTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGAc                     <  1:439007/140‑1 (MQ=255)                                                                                                                             cTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGACCACACTTAATGtt        <  2:1023656/140‑1 (MQ=255)                                                                                                                             cTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGACCACACTTAATGtt        >  1:737733/1‑140 (MQ=255)                                                                                                                             cTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGACCACACTTAATGtt        >  2:58280/1‑140 (MQ=255)                                                                                                                             cTGTCGATTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGACCACACTTAATGtt        >  2:1164511/1‑140 (MQ=255)                                                                                                                                   aTTTTTTGCTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGACCACACTTAATGTTCAACtt  >  2:976125/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     CTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGACCACACTTAATGTTCAACTT  >  NC_000913/1271832‑1272101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑