Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,331,303	T→C	L86L (TTG→CTG)	topA →	DNA topoisomerase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,331,303	0	T	C	100.0%	222.9 / NA	65	L86L (TTG→CTG)	topA	DNA topoisomerase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/33);  total (32/33)

TGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCGGGAAGTGATTGG  >  NC_000913/1331167‑1331430                                                                                                                                         |                                                                                                                                tGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGtgctgc                                                                                                                              <  2:1313374/140‑1 (MQ=255)  ggCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGcc                                                                                                                             <  1:193039/140‑1 (MQ=255)      cAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGt                                                                                                                         <  1:1131652/140‑1 (MQ=255)      cAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGt                                                                                                                         >  1:461227/1‑140 (MQ=255)        gCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTaa                                                                                                                       >  1:460806/1‑140 (MQ=255)        gCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTaa                                                                                                                       >  2:431606/1‑140 (MQ=255)         cTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTaaa                                                                                                                      >  1:44847/1‑140 (MQ=255)         cTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTaaa                                                                                                                      >  1:856564/1‑140 (MQ=255)                 aaGAGTGCCGTCTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGgt                                                                                                              <  2:906242/140‑1 (MQ=255)                 aaGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCTTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGgt                                                                                                              <  2:1299912/140‑1 (MQ=255)                 aaGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGgt                                                                                                              <  2:44847/140‑1 (MQ=255)                 aaGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGgt                                                                                                              <  2:856564/140‑1 (MQ=255)                      tGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGtct                                                                                                         >  2:568056/1‑140 (MQ=255)                       gCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGtctc                                                                                                        <  1:1289059/140‑1 (MQ=255)                        ccGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTctct                                                                                                       <  2:175413/140‑1 (MQ=255)                         cGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTctg                                                                                                      <  2:116741/140‑1 (MQ=255)                                aCCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTctgaactgaa                                                                                               >  2:983661/1‑140 (MQ=255)                                     caccaAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAaacaac                                                                                          >  1:567922/1‑140 (MQ=255)                                      accaAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGTACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACt                                                                                         >  2:535558/1‑140 (MQ=255)                                       ccaAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTg                                                                                        >  2:1231214/1‑140 (MQ=255)                                                cTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGaaaaa                                                                               >  2:206541/1‑140 (MQ=255)                                                 tAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAg                                                                              <  2:565315/140‑1 (MQ=255)                                                      aGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGAc                                                                         <  1:206541/140‑1 (MQ=255)                                                      aGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGAc                                                                         <  1:983661/140‑1 (MQ=255)                                                       gCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGAcc                                                                        <  1:1054564/140‑1 (MQ=255)                                                          tAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACcaca                                                                     >  1:262083/1‑140 (MQ=255)                                                            aaaaGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACatc                                                                   <  2:1245362/140‑1 (MQ=255)                                                              aaGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACatcta                                                                 <  2:262083/140‑1 (MQ=255)                                                                    gAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGTACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGc                                                           <  1:535558/140‑1 (MQ=255)                                                                      aCGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACATCTGGCTGAAAAAGCCGACCACATCTATCTCGCaa                                                         <  2:1304874/140‑1 (MQ=255)                                                                         tGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCg                                                      >  1:722882/1‑140 (MQ=255)                                                                           gcgcTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGAc                                                    >  1:364253/1‑140 (MQ=255)                                                                            cgcTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGAcc                                                   <  1:423600/140‑1 (MQ=255)                                                                                ctcGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGa                                               >  1:1368821/1‑140 (MQ=255)                                                                                ctcGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGa                                               <  2:461227/140‑1 (MQ=255)                                                                                ctcGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGa                                               <  1:610627/140‑1 (MQ=255)                                                                                ctcGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGACTGAAAAAGCCGACCACATCTATTTCGCAACCGACCTTGa                                               >  2:767503/1‑140 (MQ=255)                                                                                    tCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACcgc                                           >  2:320353/1‑140 (MQ=255)                                                                                     cAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACcgcg                                          <  1:1231214/140‑1 (MQ=255)                                                                                          gTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAggg                                     <  2:567922/140‑1 (MQ=255)                                                                                           tATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAgggg                                    >  1:1155909/1‑140 (MQ=255)                                                                                           tATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAgggg                                    >  1:1385168/1‑140 (MQ=255)                                                                                           tATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAgggg                                    >  1:666902/1‑140 (MQ=255)                                                                                           tATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAgggg                                    >  1:782938/1‑140 (MQ=255)                                                                                            aTGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGa                                   <  2:460806/140‑1 (MQ=255)                                                                                             tGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACcgcg                                          >  2:54076/1‑132 (MQ=255)                                                                                             tGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACcgcg                                          <  1:54076/132‑1 (MQ=255)                                                                                             tGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGaa                                  >  1:1312055/1‑140 (MQ=255)                                                                                                  gTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGa                                               >  2:192530/1‑122 (MQ=255)                                                                                                  gTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGa                                               <  1:192530/122‑1 (MQ=255)                                                                                                  gTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCAt                             >  2:1223271/1‑140 (MQ=255)                                                                                                  gTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCAt                             >  1:668973/1‑140 (MQ=255)                                                                                                     gACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGc                          >  2:318364/1‑140 (MQ=255)                                                                                                     gACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGc                          >  1:535829/1‑140 (MQ=255)                                                                                                           tGGCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGACAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCa                    <  2:1312055/140‑1 (MQ=255)                                                                                                             gCACAATTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCAcc                  <  2:782938/140‑1 (MQ=255)                                                                                                                 aaTTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCg              >  2:345461/1‑140 (MQ=255)                                                                                                                 aaTTGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCg              <  2:1368821/140‑1 (MQ=255)                                                                                                                    tGGGAGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCC‑ACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCGGGaa          >  2:1380650/1‑140 (MQ=255)                                                                                                                        aGGCGCACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCGGGAAGTg       >  2:409155/1‑140 (MQ=255)                                                                                                                           cgcACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCGGGAAGTGAtt    <  1:345461/140‑1 (MQ=255)                                                                                                                           cgcACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCGGGAAGTGAtt    <  1:1223271/140‑1 (MQ=255)                                                                                                                             cACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCGGGaa          <  2:159382/132‑1 (MQ=255)                                                                                                                             cACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCGGGaa          >  1:159382/1‑132 (MQ=255)                                                                                                                             cACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCGGGAAGTGATTgg  <  1:318364/140‑1 (MQ=255)                                                                                                                             cACTATGAAGTGCTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCGGGAAGTGATTgg  <  2:668973/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                TGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCGGGAAGTGATTGG  >  NC_000913/1331167‑1331430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑