Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,352,858	C→T	A257A (GCG→GCA)	sapD ←	putrescine ABC exporter ATP binding protein SapD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,352,858	0	C	T	100.0%	312.7 / NA	89	A257A (GCG→GCA)	sapD	putrescine ABC exporter ATP binding protein SapD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/49);  total (40/49)

CGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAA  >  NC_000913/1352721‑1352992                                                                                                                                          |                                                                                                                                       cGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAgtgc                                                                                                                                      >  1:432689/1‑140 (MQ=255)  gTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGcc                                                                                                                                     <  2:350328/140‑1 (MQ=255)  gTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGcc                                                                                                                                     <  1:501662/140‑1 (MQ=255)   ttGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCt                                                                                                                                    <  1:237679/140‑1 (MQ=255)   ttGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCt                                                                                                                                    <  1:452196/140‑1 (MQ=255)   ttGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCt                                                                                                                                    <  1:736379/140‑1 (MQ=255)   ttGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCt                                                                                                                                    <  2:5507/140‑1 (MQ=255)   ttGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCt                                                                                                                                    <  1:1145181/140‑1 (MQ=255)     gtgCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTgg                                                                                                                                  >  1:902712/1‑140 (MQ=255)     gtgCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTgg                                                                                                                                  >  2:1079539/1‑140 (MQ=255)     gtgCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTgg                                                                                                                                  <  2:431792/140‑1 (MQ=255)        cATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGta                                                                                                                               >  2:1418466/1‑140 (MQ=255)        cATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGta                                                                                                                               <  1:275715/140‑1 (MQ=255)         aTACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGtat                                                                                                                              <  2:161471/140‑1 (MQ=255)              ggCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGga                                                                                                                         <  1:851126/140‑1 (MQ=255)              ggCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGga                                                                                                                         >  1:845680/1‑140 (MQ=255)               gCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCt                                                                                                                                    <  1:1131414/128‑1 (MQ=255)               gCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCt                                                                                                                                    >  2:1131414/1‑128 (MQ=255)                cAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCt                                                                                                                                    >  2:273178/1‑127 (MQ=255)                cAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCt                                                                                                                                    <  1:273178/127‑1 (MQ=255)                cAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGgatg                                                                                                                       >  1:475317/1‑140 (MQ=255)                cAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGgatg                                                                                                                       <  2:475317/140‑1 (MQ=255)                  aCGTGGCCGCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGgatgat                                                                                                                     >  2:788083/1‑140 (MQ=255)                  aCGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGgatgat                                                                                                                     <  1:1418466/140‑1 (MQ=255)                   cGTGGCCCCAGACGACAACCAATCGGTAACTGCTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCt                                                                                                                                    >  1:579656/1‑124 (MQ=255)                   cGTGGCCCCAGACGACAACCAATCGGTAACTGCTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCt                                                                                                                                    <  2:579656/124‑1 (MQ=255)                     tGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGAtgtg                                                                                                                  >  2:45519/1‑140 (MQ=255)                     tGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGAtgtg                                                                                                                  <  2:845680/140‑1 (MQ=255)                       gCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGc                                                                                                                >  2:317521/1‑140 (MQ=255)                       gCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGc                                                                                                                >  1:1094194/1‑140 (MQ=255)                        ccccAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCa                                                                                                               <  2:1423444/140‑1 (MQ=255)                                gacAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCAcc                                                                                                       <  2:1207437/140‑1 (MQ=255)                                gacAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCAcc                                                                                                       <  2:505225/140‑1 (MQ=255)                                gacAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCAcc                                                                                                       <  1:788083/140‑1 (MQ=255)                                gacAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCAc                                                                                                        <  1:1126191/139‑1 (MQ=255)                                gacAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCAc                                                                                                        >  2:1126191/1‑139 (MQ=255)                                 acAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCa                                                                                                      <  2:674782/140‑1 (MQ=255)                                  cAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCa                                                                                                      <  2:703660/139‑1 (MQ=255)                                  cAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCa                                                                                                      >  1:703660/1‑139 (MQ=255)                                        aTCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCtt                                                                                               >  1:790316/1‑140 (MQ=255)                                        aTCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCtt                                                                                               >  2:1156397/1‑140 (MQ=255)                                        aTCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCtt                                                                                               >  1:526943/1‑140 (MQ=255)                                               aCTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGgc                                                                                        <  2:876713/140‑1 (MQ=255)                                                  gTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGcgcg                                                                                     <  2:526943/140‑1 (MQ=255)                                                   ttCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCa                                                                                                      >  2:601480/1‑122 (MQ=255)                                                   ttCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCa                                                                                                      <  1:601480/122‑1 (MQ=255)                                                   ttCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCgg                                                                                    >  2:1100608/1‑140 (MQ=255)                                                    tCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGt                                                                                   <  1:45519/140‑1 (MQ=255)                                                     ccAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGtt                                                                                  >  2:179986/1‑140 (MQ=255)                                                       agcagTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTc                                                                                <  1:1100608/140‑1 (MQ=255)                                                        gcagTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGgc                                                                                        >  2:595716/1‑131 (MQ=255)                                                        gcagTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGgc                                                                                        <  1:595716/131‑1 (MQ=255)                                                        gcagTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTcc                                                                               <  1:1156397/140‑1 (MQ=255)                                                             gggATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGt                                                                          <  1:317521/140‑1 (MQ=255)                                                              ggATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGtt                                                                         >  2:1197264/1‑140 (MQ=255)                                                                   gcgcCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGAcc                                                                    >  1:838112/1‑140 (MQ=255)                                                                    cgcCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCg                                                                   >  1:1252991/1‑140 (MQ=255)                                                                      ccGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCa                                                                              <  2:889397/127‑1 (MQ=255)                                                                      ccGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCa                                                                              >  1:889397/1‑127 (MQ=255)                                                                        gggCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGt                                                               >  1:138225/1‑140 (MQ=255)                                                                         ggCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTa                                                              >  2:338867/1‑140 (MQ=255)                                                                         ggCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTa                                                              >  1:1033736/1‑140 (MQ=255)                                                                             gCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGc                                                          >  1:1086233/1‑140 (MQ=255)                                                                              cGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCa                                                         >  1:1107916/1‑140 (MQ=255)                                                                              cGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCa                                                         >  2:1075830/1‑140 (MQ=255)                                                                              cGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCa                                                         >  2:1425722/1‑140 (MQ=255)                                                                              cGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCa                                                         >  2:957836/1‑140 (MQ=255)                                                                                       gCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAAttt                                                >  2:10545/1‑140 (MQ=255)                                                                                           cTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTGATTTTATc                                            >  2:1437966/1‑140 (MQ=255)                                                                                           cTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATc                                            <  1:1075830/140‑1 (MQ=255)                                                                                           cTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATc                                            <  1:1197264/140‑1 (MQ=255)                                                                                           cTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATc                                            <  1:957836/140‑1 (MQ=255)                                                                                           cTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGCTAATTTTATc                                            <  2:138225/140‑1 (MQ=255)                                                                                                     gCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGCTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTgg                                  >  2:471975/1‑140 (MQ=255)                                                                                                      cATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGc                                 <  1:10545/140‑1 (MQ=255)                                                                                                         cgcgCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTaa                              <  2:1257790/140‑1 (MQ=255)                                                                                                          gcgcTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTGATTTTATCCGCCCATTGGCTAAg                             <  1:1437966/140‑1 (MQ=255)                                                                                                          gcgcTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAg                             <  2:838112/140‑1 (MQ=255)                                                                                                          gcgcTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAg                             <  2:432689/140‑1 (MQ=255)                                                                                                            gcTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCa                           <  2:1107916/140‑1 (MQ=255)                                                                                                                ccGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTg                       <  2:1094194/140‑1 (MQ=255)                                                                                                                ccGAAGTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTg                       <  2:1033736/140‑1 (MQ=255)                                                                                                                     gTCTGGTATCGCGCGGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGt                  <  2:1086233/140‑1 (MQ=255)                                                                                                                               gcgcgGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGAt        <  2:1252991/140‑1 (MQ=255)                                                                                                                                cgcgGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATa       >  1:1363994/1‑140 (MQ=255)                                                                                                                                cgcgGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATa       >  2:1386376/1‑140 (MQ=255)                                                                                                                                  cgGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAg     <  1:1386376/140‑1 (MQ=255)                                                                                                                                  cgGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAg     >  1:1370581/1‑140 (MQ=255)                                                                                                                                  cgGATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAg     >  1:1360477/1‑140 (MQ=255)                                                                                                                                    gATCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCa   >  1:910217/1‑140 (MQ=255)                                                                                                                                     aTCAGTGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCaa  <  2:910217/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       CGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAA  >  NC_000913/1352721‑1352992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑